Abstract: A gene related to biosynthesis of ergothioneine is provided. Based on five synthesis genes of ergothioneine in Mycobacteroides abscessus, only an encoding sequence of each of the five genes is retained, and a gene structure of an encoding region in each gene is optimized to obtain genes related to the biosynthesis of the ergothioneine that are stably expressed in rice. These genes have sequences set forth in SEQ ID NO: 1 to SEQ ID NO: 5, and encoded proteins thereof have amino acid sequences set forth in SEQ ID NO: 6 to SEQ ID NO: 10. The gene is transferred into rice to construct a transgenic rice molecular farm to produce the ergothioneine. As a molecular farm, rice seeds do not contain alkaloids or allergens that are harmful to the human body. Moreover, biosynthesized ergothioneine shows the advantages of easy extraction and processing and of low production cost.

Abstract: The present disclosure provides a gene combination for expressing and producing terrequinone A in Escherichia coli and use thereof. The gene combination includes a tdiAS gene, a tdiBS gene, a tdiCS gene, a tdiDS gene, a tdiES gene, an sfpS gene, an ScCKS gene, and an AtIPKS gene with nucleotide sequences set forth in SEQ ID NOS:1 to 8. In the present disclosure, a recombinant engineered strain capable of producing terrequinone A having anti-cancer activity is obtained by separately constructing recombinant plasmids pC02 and pU03 through the eight genes and transforming the two recombinant plasmids into E. coli. The content of terrequinone A in a fermentation broth thereof is 106.3 mg/L, which has potential application value in the biopharmaceutical field.

Abstract: Autotrophic nitrogen fixation genes and an autotrophic nitrogenase expression cassette for plants and uses thereof are disclosed. Nitrogenase related genes of nitrogen fixation gene cluster from Paenibacillus sp. are specifically optimized by plant expression patterns through synthetic biology, to obtain autotrophic nitrogen fixation genes, which are then constructed into plant constitutive expression units. The expression units are assembled in a plant expression vector by isocaudarner cloning method and transformed into plants, thereby realizing autotrophic nitrogen fixation of the plants.

Abstract: Autotrophic nitrogen fixation genes and an autotrophic nitrogenase expression cassette for plants and uses thereof are disclosed. Nitrogenase related genes of nitrogen fixation gene cluster from Paenibacillus sp. are specifically optimized by plant expression patterns through synthetic biology, to obtain autotrophic nitrogen fixation genes, which are then constructed into plant constitutive expression units. The expression units are assembled in a plant expression vector by isocaudarner cloning method and transformed into plants, thereby realizing autotrophic nitrogen fixation of the plants.