Abstract: An underneath large-capacity single-gas-pump foam generating device includes a liquid replenishment mechanism and a foam generating mechanism. The liquid replenishment mechanism includes a liquid replenishment groove and a liquid replenishment bottle. The liquid replenishment bottle has a bottle opening sleeved in the liquid replenishment groove. The foam generating mechanism includes a gas supply pump unit, a gas mixing groove cavity, a gas-liquid mixing structure, a gas inlet and a vent valve structure. A lower portion of the gas mixing groove cavity is communicated with a lower portion of the liquid replenishment groove, a liquid replenishment one-way valve is arranged between the gas mixing groove cavity and the liquid replenishment groove. The gas-liquid mixing structure is provided with a liquid outlet channel, a gas outlet channel and a mixing channel, an inlet end of the liquid outlet channel extends downwardly into a bottom portion of the gas mixing groove cavity.

Abstract: A method for identifying and characterizing biological parts based on omics datasets and a dual-fluorescent reporter gene system, and a biological part library constructed thereon are provided, relating to a technical filed of biology. The method includes steps of: identifying the biological parts using the omics datasets; constructing a single-fluorescent reporter gene system using a shuttle vector pEZ15Asp as a skeleton for screening and determining fluorescent reporter genes; obtaining a dual-fluorescent reporter gene system skeleton; constructing recombinant plasmids, and finally transforming into competent cells for quantitative analysis of fluorescence intensities. The present invention is convenient and quick, and can screen and identify different biological parts such as RBS, UTRs, promoters, and terminators of different intensities in batch quantitatively in a relatively short time. Moreover, the present invention can quickly expand the biological part library of Z.

Abstract: A method for identifying and characterizing biological parts based on omics datasets and a dual-fluorescent reporter gene system, and a biological part library constructed thereon are provided, relating to a technical filed of biology. The method includes steps of: identifying the biological parts using the omics datasets; constructing a single-fluorescent reporter gene system using a shuttle vector pEZ15Asp as a skeleton for screening and determining fluorescent reporter genes; obtaining a dual-fluorescent reporter gene system skeleton; constructing recombinant plasmids, and finally transforming into competent cells for quantitative analysis of fluorescence intensities. The present invention is convenient and quick, and can screen and identify different biological parts such as RBS, UTRs, promoters, and terminators of different intensities in batch quantitatively in a relatively short time. Moreover, the present invention can quickly expand the biological part library of Z.