Abstract: A microfluidic ion detection chip having a bubble brightening structure includes a substrate, an upper plate, two glass cover plates, and a foam board. The substrate is provided with a fluid mixing region, a lower optical detection through hole, and a lower gas flow channel. The upper plate is adhesively connected to the substrate and is provided with a sample outlet, an upper optical detection through hole, an upper gas flow channel, and two sample inlets, the upper gas flow channel and the lower gas flow channel are combined to form a gas flow channel. The two glass cover plates are respectively disposed at the lower optical detection through hole and the upper optical detection through hole. The foam board is provided with a foaming structure capable of generating bubbles and a surfactant coated on the foam board, the foam board is disposed at the gas flow channel.