Abstract: A device is provided for the detection of multiple analytes in at least one sample. The device contains a solid substrate with a test surface. On the test surface is defined at least one reaction area containing at least one array of discrete test sites. Each of these test sites have a test molecule immobilized on to it, and different test sites may have different test molecules immobilized thereon. A divider is provided for attachment onto the solid substrate. The divider contains a plurality of holes provided on an attachment surface. The attachment surface is complementary to the test surface of the solid device and is adapted for reversible attachment thereto such that when the two parts are attached, each of the holes is adjoined with a portion of the test surface to create a plurality of leak-proof chambers. The test surface within the chamber contains a plurality of test sites exposed within the chambers.