Abstract: An LC-MS/MS method for detecting 11 vitamins D in blood is provided. The 11 vitamins D in the invention include D2, D3, 1,25-(OH)2D2, 1,25-(OH)2D3, 25-(OH)D2, 25-(OH)D3, 3-epi-25-(OH)D2, 3-epi-25(OH)D3, 24,25-(OH)2D2, 24,25-(OH)2D3 and 3-epi-24,25-(OH)2D3. The method of the invention includes: preparing a standard curve equation, pre-treating a sample to be detected, and detecting the incoming sample. The pre-processing of the invention adopts the combination of protein precipitation, liquid-liquid extraction and derivation, so that the 11 vitamins D are extracted by one injection, and a pg-level sensitivity of the detection method is ensured at the same time. Moreover, the detection method of the invention also has the technical advantage of high accuracy.

Abstract: Provided is a method for simultaneously detecting Vitamin K1 and Vitamin K2 in traces of blood. The method includes: constructing a two-dimensional liquid chromatography-tandem mass spectrometer, establishing an analytical method, and detecting at least three mixed standard solutions using the constructed two-dimensional liquid chromatography-tandem mass spectrometer to obtain a first detection result; fitting standard curve equations respectively corresponding to Vitamin K1 and Vitamin K2; and mixing and centrifuging a blood sample to which an extraction reagent and a certain amount of internal standard substance are added, collecting a supernatant, blowing the supernatant to dry with nitrogen, redissolving the residue, and detecting the dry supernatant using the constructed two-dimensional liquid chromatography-tandem mass spectrometer to obtain a second detection result. In this manner, concentrations of Vitamin K1 and Vitamin K2 in the blood sample are obtained.

Abstract: Provided is a method for simultaneously detecting Vitamin K1 and Vitamin K2 in traces of blood. The method includes: constructing a two-dimensional liquid chromatography-tandem mass spectrometer, establishing an analytical method, and detecting at least three mixed standard solutions using the constructed two-dimensional liquid chromatography-tandem mass spectrometer to obtain a first detection result; fitting standard curve equations respectively corresponding to Vitamin K1 and Vitamin K2; and mixing and centrifuging a blood sample to which an extraction reagent and a certain amount of internal standard substance are added, collecting a supernatant, blowing the supernatant to dry with nitrogen, redissolving the residue, and detecting the dry supernatant using the constructed two-dimensional liquid chromatography-tandem mass spectrometer to obtain a second detection result. In this manner, concentrations of Vitamin K1 and Vitamin K2 in the blood sample are obtained.