Abstract: The present invention relates to the preparation and use of a cell cryopreservation formulation convenient for clinical use. In particular, provided is a cell cryopreservation (freezing) formulation, comprising: (1) a cell; (2) a cryopreservation diluent; the cryopreservation diluent comprising: an aqueous solution of sodium chloride, protective proteins, and dimethyl sulfoxide. The invention also relates to a corresponding cell resuscitation method. The invention adopts a no-wash cryopreservation solution for preparation of a cell frozen preparation, and the cell resuscitation and dilution for clinical use can be carried out by a simple formulating operation.