Abstract: The present invention provides an inhalation spray of Semaglutide with good patient compliance and high bioavailability at the targeted site, which belongs to the technical field of pharmaceutical preparation. The inhalation spray of Semaglutide according to the invention comprising, by weight to volume, 0.01% to 20% of Semaglutide in the inhalation spray, wherein the proportion of fine particles with a particle size less than 5 ?m formed after the inhalation spray is atomized by a spray device is not less than 10%. The inhalation spray of Semaglutide according to the invention has a simple formulation, low toxic and side effects, atomization administration of the medicinal liquid through an inhalation device, uniform distribution of spray droplets, a high lung deposition rate, low irritation and convenient administration, which is suitable for industrial production. The invention further provides a preparation method of the inhalation spray of Semaglutide.