Abstract: In the present disclosure, there is provided an engineered Cas12f1 guide RNA for increasing intracellular gene editing activity of a CRISPR/Cas12f1 system by overcoming limitations of the prior art. The engineered Cas12f1 guide RNA is an engineered form of a naturally occurring guide RNA whose structure is partially modified. In addition, the engineered Cas12f1 guide RNA is characterized in that at least a portion of the scaffold region, which serves to interact with the Cas12f1 protein, is modified. The engineered scaffold region is characterized in that it is different from a scaffold region of a naturally occurring guide RNA.

Abstract: Disclosed in the present specification are: dead Cas12f1 having the nucleic acid cleavage activity removed; and a fusion protein in which a functional domain is fused to the dead Cas12f1. The dead Cas12f1 and the dCas12f1-based fusion protein may: form a CRISPR gene-editing system together with a guide RNA; and exhibit various functions relating to a target gene such as base editing and expression control.

Abstract: The present disclosure relates to an aglycosylated antibody-producing transgenic mouse and the use of an aglycosylated antibody produced therefrom. When the transgenic mouse of the present disclosure is used, it is possible to easily produce aglycosylated antibodies against various target antigens, and to precisely diagnose disease by detecting a glycoprotein biomarker using the produced aglycosylated antibody.

Abstract: A CRISPR regulatory system is disclosed. More particularly, the CRISPR regulatory system is useful for effectively regulating expression of a target gene and contains a Cas12f1 fusion protein and an engineered Cas12f1 guide RNA Uses of the CRISPR regulatory system are also disclosed. A method of regulating expression of a target gene by using a CRISPR regulatory system containing a Cas12f1 fusion protein and an engineered Cas12f1 guide RNA is also disclosed.

Abstract: Provided is an engineered CRISPR/Cas12f1 (Cas14a1) system. Provided are an engineered guide RNA for more effectively performing cleavage, editing, or modifying of a target nucleic acid, and an engineered CRISPR/Cas12f1 (Cas14a1) system containing the engineered guide RNA. In addition, provided is a method of performing cleavage, editing, or modifying of a target nucleic acid by using the engineered guide RNA and the engineered CRISPR/Cas12f1 (Cas14a1) system including the engineered guide RNA.

Abstract: In the present disclosure, there is provided an engineered Cas12f1 guide RNA for increasing intracellular gene editing activity of a CRISPR/Cas12f1 system by overcoming limitations of the prior art. The engineered Cas12f1 guide RNA is an engineered form of a naturally occurring Cas12f1 guide RNA to which a new component is added and also in which a part of its structure is modified. The engineered Cas12f1 guide RNA is characterized by comprising a U-rich tail, which is a new component, at the 3? end. In addition, the engineered Cas12f1 guide RNA is characterized in that at least a portion of the scaffold region, which serves to interact with the Cas12f1 protein, is modified.

Abstract: The present invention relates to a composition for genome editing using a CRISPR/Cpf1 system and a use thereof and, more particularly, to a composition for genome editing comprising: a CRISPR RNA (crRNA) including a guide sequence capable of hybridizing with a target nucleotide sequence, and a uridine repeat sequence connected to the 3?-end of the guide sequence, or a DNA encoding the same; and a Cpf1 protein or a DNA encoding the same, a method for genome editing using the same, a method for construction of a genetically modified organism, and a genetically modified organism. The present invention can increase an indel efficiency and decrease off-target activity in genome editing of eukaryotic cells using the CRIPSPR/Cpf1 system and thus can easily construct a genetically modified cell or genetically modified animal or plant having a desired gene inserted thereinto (knock-in) or deleted therefrom (knock-out).

Abstract: Provided are an engineered CRISPR/Cas12f1 system of which editing efficiency is improved compared to a canonical CRISPR/Cas12f1 system, and a use thereof. A U-rich tail sequence in crRNA is provided that can be introduced into an original guide RNA to improve the editing efficiency of the CRISPR/Cas12f1 system. The U-rich tail sequence has abundant uridine residues at the 3?-ternimus of crRNA, and may further include additional nucleic acids in addition to uridine depending on the environment in which the guide RNA is actually used and the environment in which the guide RNA is expressed. The inventors of the present application described the structure of the U-rich tail sequence, the position of introduction, and the effect thereof with respect to genome editing efficiency.

Abstract: The present disclosure relates to an aglycosylated antibody-producing transgenic mouse and the use of an aglycosylated antibody produced therefrom. When the transgenic mouse of the present disclosure is used, it is possible to easily produce aglycosylated antibodies against various target antigens, and to precisely diagnose disease by detecting a glycoprotein biomarker using the produced aglycosylated antibody.

Abstract: The present invention relates to a composition for genome editing using a CRISPR/Cpf1 system and a use thereof and, more particularly, to a composition for genome editing comprising: a CRISPR RNA (crRNA) including a guide sequence capable of hybridizing with a target nucleotide sequence, and a uridine repeat sequence connected to the 3?-end of the guide sequence, or a DNA encoding the same; and a Cpf1 protein or a DNA encoding the same, a method for genome editing using the same, a method for construction of a genetically modified organism, and a genetically modified organism. The present invention can increase an indel efficiency and decrease off-target activity in genome editing of eukaryotic cells using the CRIPSPR/Cpf1 system and thus can easily construct a genetically modified cell or genetically modified animal or plant having a desired gene inserted thereinto (knock-in) or deleted therefrom (knock-out).

Abstract: A rechargeable battery includes a case, an electrode assembly disposed in the case, and an insulating member disposed in the case, the insulating member being fixed to the electrode assembly, the insulating member including a first portion and a second portion, the second portion extending from the first portion and contacting the electrode assembly at a coated region of the electrode assembly.

Abstract: A rechargeable battery includes an electrode assembly including first and second electrodes, a case having the electrode assembly embedded therein, a cap plate coupled to an opening of the case, and an insulating member between the electrode assembly and the case. The insulating member includes a first support frame and a second support frame that are spaced apart from each other and an insulating film attached to the first and second support frames.

Abstract: A rechargeable battery includes an electrode assembly that performs charging and discharging operations, a case that accommodates the electrode assembly therein, a cap plate coupled to the case, an electrode terminal connected to the electrode assembly and mounted in a terminal hole of the cap plate, and an insulation member that coats the case. The insulation member has a protruding portion that protrudes from an external circumference of the cap plate along a surface of the cap plate. The protruding portion has an exhaust groove on at least one side thereof.

Abstract: An RFID reader control system and method is provided. A protocol for controlling an RFID reader and an RFID reader control unit of a mobile phone is defined. Messages, information, commands, responses, and notification are constructed and transmitted between the RFID reader and the RFID reader control unit.

Abstract: An RFID reader control system and method is provided. A protocol for controlling an RFID reader and an RFID reader control unit of a mobile phone is defined. Messages, information, commands, responses, and notification are constructed and transmitted between the RFID reader and the RFID reader control unit.

Abstract: A battery includes a case accommodating an electrode assembly, the case including an opening at one end and including at least one sidewall, the sidewall having a coupling portion adjacent to the opening, a cap plate that closes the opening, the cap plate having an upper surface, the coupling portion of the sidewall connecting the cap plate to the case, the coupling portion including a top surface of the sidewall, the cap plate overlying a first portion of the top surface of the sidewall, such that the upper surface of the cap plate is entirely above top surface of the sidewall, and a welding bead contacting the cap plate and a second portion of the top surface of the sidewall, the second portion of the top surface being between the first portion and an outer surface of the sidewall.

Abstract: A rechargeable battery includes an electrode assembly in a battery case, and a buffer sheet between the electrode assembly and the battery case, the buffer sheet contacting the electrode assembly and the battery case.

Abstract: A rechargeable battery includes an electrode assembly including a first electrode, a second electrode, and a separator between the first electrode and the second electrode. The rechargeable battery also includes a case accommodating the electrode assembly. The rechargeable battery further includes a cap plate including a first plate and a second plate coupled to the first plate along a joint. The cap plate is coupled to an opening of the case. The rechargeable battery also includes a vent unit at the joint where the first plate of the cap plate is coupled to the second plate.

Abstract: A battery module includes an electrode assembly in a case, at least one current collector in the case, the current collector being electrically connected to the electrode assembly, a cap plate covering the case, the cap plate including a safety vent and at least one terminal inserter penetrating the cap plate, at least one terminal part including a terminal plate outside the case and a connector connecting the at least one current collector to the terminal plate through the at least one terminal inserter, at least one fixing member fixing the terminal part into the cap plate, and a groove pattern in an area of the cap plate adjacent to the safety vent.

Abstract: A rechargeable battery including an electrode assembly performing charging and discharging; a case accommodating the electrode assembly; a cap plate coupled with the case; an electrode terminal in a terminal hole of the cap plate, the electrode terminal being electrically connected to the electrode assembly; and an insulator electrically insulating the electrode terminal and the cap plate, wherein the insulator includes a first member between an outside of the cap plate and a surface of the electrode terminal facing the cap plate, and a second member enclosing an outer surface of the electrode terminal and the first member and being coupled with the first member with a step structure.