Abstract: The present disclosure provides a ceramic material for a thermal barrier coating and a manufacturing method thereof. A chemical composition of the ceramic material is LaYbZrCeO7. The ceramic material is manufactured by doping LaO1.5, YbO1.5 and CeO2 into ZrO2. A mole ratio of LaO1.5, YbO1.5, CeO2 and ZrO2 is 1:1:1:1. The manufactured ceramic material is in a composite phase structure mainly including a pyrochlore phase and a fluorite phase. The ceramic material according to the present disclosure can effectively inhibit corrosion penetration of molten CMAS in a high temperature environment, which reduces or avoids ceramic cracking and peeling. This better maintains microstructural integrity of the ceramic surface, thereby extending service life of ceramics.

Abstract: The present invention relates to an adeno-associated virus with site-directed mutagenesis and site-specific modification, and a preparation method and uses thereof. Specifically, the present invention uses genetic code expansion techniques to incorporate non-natural amino acid into an adeno-associated virus capsid protein VP1 or fragment thereof, thereby obtaining an adeno-associated virus with site-directed mutagenesis using the non-natural amino acid. The adeno-associated virus with site-directed mutagenesis is equivalent to a wild-type virus in terms of production, transduction and mobility, can couple with other functional molecules, such as targeting molecules, and can carry a functional gene in a normal manner, which indicates that the adeno-associated virus with site-directed mutagenesis can be used as a tool adeno-associated virus, and applied in various aspects associated with adeno-associated virus such as finding adeno-associated virus binding proteins or using as target genetic therapy vector.

Abstract: The present invention relates to an adeno-associated virus with site-directed mutagenesis and site-specific modification, and a preparation method and uses thereof. Specifically, the present invention uses genetic code expansion techniques to incorporate non-natural amino acid into an adeno-associated virus capsid protein VP1 or fragment thereof, thereby obtaining an adeno-associated virus with site-directed mutagenesis using the non-natural amino acid. The adeno-associated virus with site-directed mutagenesis is equivalent to a wild-type virus in terms of production, transduction and mobility, can couple with other functional molecules, such as targeting molecules, and can carry a functional gene in a normal manner, which indicates that the adeno-associated virus with site-directed mutagenesis can be used as a tool adeno-associated virus, and applied in various aspects associated with adeno-associated virus such as finding adeno-associated virus binding proteins or using as target genetic therapy vector.