Abstract: Disclosed herein are an isolated polynucleotide comprising a lectin gene regulation site of a mud loach, expressed as SEQ ID NO: 1, an expression vector comprising a lectin gene regulation site of a mud loach, an expression vector comprising a lectin gene regulation site of a mud loach and a growth hormone gene of a mud loach, and an expression vector comprising a lectin gene regulation site of a mud loach and a growth hormone gene of a carp. Also provided a method of making a transgenic mud loach or carp comprising microinjecting the expression vector of a growth hormone gene into fertilized eggs of a mud loach or carp and culturing the eggs such that the eggs hatch and result in a mud loach or carp fish which expresses the growth hormone gene at levels which increase the rate of growth of the fish relative to wild-type mud loach or carp, and a mud loach or carp transformed with the expression vector.

Abstract: Disclosed herein are an isolated polynucleotide comprising a lectin gene regulation site of a mud loach, expressed as SEQ ID NO: 1, an expression vector comprising a lectin gene regulation site of a mud loach, an expression vector comprising a lectin gene regulation site of a mud loach and a growth hormone gene of a mud loach, and an expression vector comprising a lectin gene regulation site of a mud loach and a growth hormone gene of a carp. Also provided a method of making a transgenic mud loach or carp comprising microinjecting the expression vector of a growth hormone gene into fertilized eggs of a mud loach or carp and culturing the eggs such that the eggs hatch and result in a mud loach or carp fish which expresses the growth hormone gene at levels which increase the rate of growth of the fish relative to wild-type mud loach or carp, and a mud loach or carp transformed with the expression vector.

Abstract: Disclosed herein are an expression vector of mud loach growth hormone gene and a fast-growing transgenic mud loach transformed with the expression vector. Also disclosed herein is an expression vector containing &bgr;-actin gene regulation site of mud loach which is constructed for expression of useful genes in fishes. The transgenic mud loaches transformed with the expression vector of mud loach growth hormone gene show growth rate of 25 times higher than that of normal mud loaches.