Abstract: The present invention relates to an in vitro liver disease model using triple co-culture and a preparation method thereof, and the method of preparing the in vitro liver disease model includes directly co-culturing hepatocytes and hepatic stellate cells, and simultaneously indirectly co-culturing Kupffer cells by being separated from the hepatocytes and the hepatic stellate cells to enable a rapid and highly accurate analysis by similarly implementing the in vivo environment compared to mono-culture, and simultaneously make it easier to collect and measure specimens than typical direct co-culture.

Abstract: The present invention provides methods for reducing amyloid beta formation and for treating diseases associated with IC the accumulation of amyloid beta, The present invention provides (1) A ? aggregation inhibition by A ? Oligomer/Fibril formation inhibition, (2) BACE-1 reduced a through ?-Amyloidogenic Processing inhibition, (3) cerebral blood flow to the increase of the cell outer A? Monomer, Oligomer & A? Fibril/Plaque reduction, (4) NO/cGMP/PKG/CREB Pathway to the activation of Neuronal cell Death inhibition and Neurogenesis, Synaptogenesis, Angiogenesis promotion, (5) DKK-1 inhibition by Wnt Signaling in the activation of synaptic plasticity recovery and A? production Positive Feedback Loop for inhibition of APP generates reduced and A? accumulation suppression, (6) Autophagy activation by cells within Toxic Mirodenafil, Sildenafil, Vardenafil, Tadalafil, Udenafil, Dasantafil, and Avanafil for the treatment of inhibition of A? Fibril/Plaque formation through removal of Soluble A? Oligomer; and a Pha