Abstract: Microfluidic devices including a microfluidic channel with at least one surface having a plurality of chiral nanoparticles disposed thereon include a light-absorbing material (e.g., gold) and a targeting ligand capable of binding to a bioactive target analyte in a biological fluid sample. The bioactive target analyte can indicate presence of cancerous cells or mutated proteins in the biological fluid sample taken from a subject. Chiral gold nanoparticle layer-by-layer assembled onto a microfluidic device can rapidly isolate and profile cancer-associated exosomes directly from blood plasma using their own unique chiral signal. Exosomes from lung cancer patients can be distinguished from healthy donor exosomes by chiroptical spectroscopic signatures of biomolecular components. Mutation/deletion of epidermal growth factor receptor are also characterized, suggesting the possibility for in-depth mutation profiling in addition to cancer diagnostics.

Abstract: The present disclosure provides porous three dimensional polymeric devices useful for purifying and/or isolating a target cell-derived component (e.g., extracellular vesicles), and methods of making and systems or kits comprising thereof. Wherein the three-dimensional device is a cube that comprises a polymer, and wherein the polymer comprises polydimethylsiloxane (PDMS).

Abstract: A separation container includes a separation tube receiving a sample therein, a first sedimentation part connected to an end portion of the tube, a particle in the sample being deposited by a centrifugal or agitating force, and a separating part provided in the tube and including at least one separating layer which selectively opens and closes the tube.

Abstract: A separation container includes a separation tube receiving a sample therein, a first sedimentation part connected to an end portion of the tube, a particle in the sample being deposited by a centrifugal or agitating force, and a separating part provided in the tube and including at least one separating layer which selectively opens and closes the tube.