Abstract: This invention provides a primer used for a next-generation sequencer that can provide a large number of reads. On the basis of the sequence: 5?-CAAGCAGAAGACGGCATACGAGAT-N5 to 15-GTCTCGTGGGCTCGGAGATGTGTATAAGA-GACAG-3?, wherein N5 to 15 indicates an index sequence of 5 to 15 nucleotides), an index sequence is designed as a nucleotide sequence exhibiting the putative number of reads, which is calculated using the estimation formula designating the number of reads as a purpose variable and the type of nucleotides in the index sequence as an explanatory variable, exceeding a given level.

Abstract: This invention provides a primer used for a next-generation sequencer that can provide a large number of reads. On the basis of the sequence: 5?-CAAGCAGAAGACGGCATACGAGAT-N5 to 15-GTCTCGTGGGCTCGGAGATGTGTATAAGA-GACAG-3?, wherein N5 to 15 indicates an index sequence of 5 to 15 nucleotides), an index sequence is designed as a nucleotide sequence exhibiting the putative number of reads, which is calculated using the estimation formula designating the number of reads as a purpose variable and the type of nucleotides in the index sequence as an explanatory variable, exceeding a given level.

Abstract: This invention provides a DNA probe that is applicable to a DNA library prepared in a simple manner with excellent reproducibility. Such DNA probe is produced by a method comprising steps of performing a nucleic acid amplification reaction in a reaction solution containing genomic DNA and a random primer at a high concentration, so as to obtain a DNA fragments with the use of the genomic DNA as a template; determining the nucleotide sequence of the resulting DNA fragments; and, on the basis of the nucleotide sequence of the DNA fragments obtained in the step above, designing a DNA probe used for detecting a DNA fragment.

Abstract: A DNA library with excellent reproducibility is readily produced. A nucleic acid amplification reaction is conducted in a reaction solution containing genomic DNA and a random primer at a high concentration to obtain a DNA fragment by the nucleic acid amplification reaction using the genomic DNA as a template.

Abstract: Manufacture or use of a mutant prenyl diphosphate synthase in which the amino acid residue located at the fifth position in the N-terminal direction from D of the N-terminal of the aspartic acid-rich domain DDXX(XX)D (the two X's in the parentheses may not be present) present in the second region among the conserved regions of the prenyl diphosphate synthase has been substituted by another amino acid.

Abstract: Manufacture or use of a mutant prenyl diphosphate synthase in which the amino acid residue located at the fifth position in the N-terminal direction from D of the N-terminal of the aspartic acid-rich domain DDXX(XX)D (the two X's in the parentheses may not be present) present in the second region among the conserved regions of the prenyl diphosphate synthase has been substituted by another amino acid.

Abstract: DNA coding for thermostable geranylgeranyl diphosphate (GGDP) synthase derived from Sulfolobus acidocaldarius is provided. The DNA is useful for production of GGDP synthase, which is, in turn, useful for production of GGDP.