Abstract: Provided is a method of easily producing a non-oriented electrical steel sheet that contains substantially no Al and contains large amounts of Si and Mn and has low iron loss, comprising hot rolling a slab having a specified chemical composition to obtain a hot-rolled sheet; coiling the hot-rolled sheet; cold rolling the hot-rolled sheet once or twice with intermediate annealing being performed therebetween, to obtain a cold-rolled sheet; and subjecting the cold-rolled sheet to final annealing, wherein the hot-rolled sheet after the hot rolling is cooled at an average cooling rate from 800° C. to 650° C. of 30° C./s or more, and thereafter the coiling is performed at 650° C. or less.

Abstract: Very simple, highly sensitive detection or quantification of target nucleic acids of interest has been achieved by: hybridizing mask oligonucleotides to regions in a single-stranded region of a nucleic acid to be assayed between which a region to be hybridized by an oligonucleotide probe is positioned, thereby opening the probe-hybridizing region and keeping the single-stranded region of the target nucleic acid stable, and then subjecting this nucleic acid having the single-stranded region to nucleic acid chromatography.

Abstract: Very simple, highly sensitive detection or quantification of target nucleic acids of interest has been achieved by: hybridizing mask oligonucleotides to regions in a single-stranded region of a nucleic acid to be assayed between which a region to be hybridized by an oligonucleotide probe is positioned, thereby opening the probe-hybridizing region and keeping the single-stranded region of the target nucleic acid stable, and then subjecting this nucleic acid having the single-stranded region to nucleic acid chromatography.

Abstract: A housing for an immunochromatography apparatus capable of detecting the concentration of an analyte contained in a sample even if it is relatively low in concentration is provided. In the housing 1 of immunochromatography apparatus having an observation window 5, the observation window 5 of the housing 1 is made of a colored transparent plastic material having a transmittance of at least 20%, preferably at least 40%, so that a detection line displayed on a developing strip 2 contained therewithin can be seen therethrough, the other portion of the housing than the observation window 5 is made of a colored transparent plastic material having a transmittance of at least 12%, and the observation window 5 and the housing 1 are simultaneously and integrally molded out of the same plastic material.