Patents by Inventor Yoshihiko Umegae
Yoshihiko Umegae has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 8999124Abstract: A detection device that is used to detect a sample includes: a base component having on its surface a sample supply position to which the sample is supplied; an electrode system formed at a distance from the sample supply position on the surface of the base component; a sliding component having a slide body that performs a sliding movement over the surface of the base component, a sample receptacle portion provided in a portion of the slide body; and a supporting portion that is fixed to the base component and supports the sliding component such that it can perform the sliding movement relative to the base component. The base and the sliding components can perform the sliding movement within a range that includes an overlap position, where the sample receptacle portion overlaps with the electrode system, and the sample supply position.Type: GrantFiled: March 2, 2011Date of Patent: April 7, 2015Assignee: Nippon Kayaku Kabushiki KaishaInventors: Takao Yokoyama, Reiko Machida, Yayoi Irie, Yoshihiko Umegae
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Patent number: 8951395Abstract: A biosensor for electrochemically measuring a sample may include a first member made of an insulating material, an electrode system including a working electrode and a counter electrode formed on the first member, a second member fixed over the first member, a sample flow channel provided between the first member and the second member, a hydrophilic section provided on at least a part of the internal surface of the sample flow channel and extending from a first end near the electrode system to a second end on the opposite side, and a flow stop area provided on a section adjacent to the second end on the first member or on the internal surface of the sample flow channel.Type: GrantFiled: November 5, 2008Date of Patent: February 10, 2015Assignee: Nippon Kayaku Kabushiki KaishaInventors: Reiko Machida, Takao Yokoyama, Yayoi Irie, Hisako Takagi, Yoshihiko Umegae, Toshio Tanabe
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Patent number: 8883439Abstract: The invention is a method for determining the concentration of an analyte in whole blood, wherein the analyte is a component which is contained in the blood, is different from a component occurring only in a red blood cell, and can generate hydrogen peroxide upon the reaction with an oxidase. Whole blood is utilized in the method. The method comprises the steps of hemolyzing the whole blood and detecting hydrogen peroxide generated by the reaction between the analyte and the oxidase. The measurement method can avoid the inhibition of color development by hemoglobin and the interference with the measurement by hemoglobin. Further it can be used for biological tests that are carried out in a household, an individual doctor's clinic or at the bedside of patients without the need for any blood cell separation procedure or the like, because the measurement utilizes whole blood.Type: GrantFiled: July 22, 2009Date of Patent: November 11, 2014Assignee: Nippon Kayaku Kabushiki KaishaInventors: Reiko Machida, Yayoi Irie, Masahiko Yabuuchi, Yoshihiko Umegae
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Patent number: 8753832Abstract: In quickly assaying a blood component interfered by glucose and/or its derivative on the bedside or in a clinic or in assaying the same by a patient in his/her own home, there has been required an assay method wherein the whole blood can be used as a sample as such without resorting to a centrifuge or the like. A method of assaying a blood component to be used for assaying a blood component interfered by glucose and/or its derivative, characterized by comprising bringing the whole blood into contact with a substance capable of converting glucose and/or its derivative into another substance not interfering the assay and, simultaneously or subsequently, separating blood cells; a device to be used in the assay method; and a kit containing this device.Type: GrantFiled: June 12, 2006Date of Patent: June 17, 2014Assignee: Nippon Kayaku Kabushiki KaishaInventors: Yoshihiko Umegae, Reiko Machida, Yayoi Irie, Toshio Tanabe
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Patent number: 8658012Abstract: The biosensor has an electrode system for electrochemically measuring 1,5-anhydroglucitol (1,5-AG) and a reagent layer formed on said electrode system. The reagent layer contains an enzyme for measuring 1,5-anhydroglucitol, phenothiazine compounds, a stabilizer selected from a group of compounds comprising metal salts, organic acids, and amino acids, and an acidic polymer compound as an optional ingredient. The biosensor has excellent storage stability and can electrochemically measure 1,5-anhydroglucitol unaffected by the hematocrit contained in a whole blood sample.Type: GrantFiled: December 7, 2009Date of Patent: February 25, 2014Assignee: Nippon Kayaku Kabushiki KaishaInventors: Takao Yokoyama, Hisako Takagi, Yayoi Irie, Reiko Machida, Yoshihiko Umegae
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Patent number: 8574882Abstract: A protein comprising an amino acid sequence having at least one mutation selected from a Gly-4 to Ala mutation, a Glu-6 to His mutation, a Ser-14 to Thr mutation, an Ala-37 to Thr or Arg mutation, a Pro-50 to Gln mutation, a Glu-67 to Gly mutation, an Asp-80 to Tyr mutation, a Val-93 to Met mutation, an Arg-156 to Pro mutation, a Leu-164 to Met mutation, an Asn-202 to Asp mutation, a Thr-235 to Ala mutation, an Asn-348 to Tyr mutation, a Gly-362 to Arg mutation and a Val-473 to Ala mutation in the amino acid sequence depicted in SEQ II NO:4. (2) A thermostable protein which comprises an amino acid sequence derived from the amino acid sequence having at least one variation described in (1) and having 1,5-anhydroglucitol dehydrogenase activity. These proteins act specifically on 1,5-anhydroglucitol (1,5-AG), have thermal stability and exhibit excellent storage stability.Type: GrantFiled: June 18, 2009Date of Patent: November 5, 2013Assignee: Nippon Kayaku Kabushiki KaishaInventors: Hideki Yoshioka, Shuhei Tsukamoto, Minoru Masuda, Reiko Machida, Yoshihiko Umegae, Masahiko Yabuuchi
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Patent number: 8465940Abstract: By a method for measuring 1,5-anhydroglucitol, comprising the steps of: eliminating or converting glucose interfering with the measurement of 1,5-anhydroglucitol and/or a derivative thereof beforehand; and measuring 1,5-anhydroglucitol performed thereafter, wherein such glucose and/or a derivative thereof are/is eliminated or converted in whole blood as such without performing blood cell separation, an enzyme for measuring 1,5-anhydroglucitol is allowed to act on without performing blood cell separation, and 1,5-anhydroglucitol is electrochemically measured, it becomes possible to measure 1,5-anhydroglucitol using a small amount of whole blood without resort to a centrifuge or the like. Accordingly, this measurement method can be applied to rapid measurement of 1,5-anhydroglucitol at bedside or in a medical examination room or to home self-measurement thereof by a patient.Type: GrantFiled: December 13, 2007Date of Patent: June 18, 2013Assignee: Nippon Kayaku Kabushiki KaishaInventors: Yoshihiko Umegae, Reiko Machida, Hisako Takagi, Yayoi Irie, Takao Yokoyama, Toshio Tanabe
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Publication number: 20120325658Abstract: A detection device that is used to detect a sample includes: a base component having on its surface a sample supply position to which the sample is supplied; an electrode system formed at a distance from the sample supply position on the surface of the base component; a sliding component having a slide body that performs a sliding movement over the surface of the base component, a sample receptacle portion provided in a portion of the slide body; and a supporting portion that is fixed to the base component and supports the sliding component such that it can perform the sliding movement relative to the base component. The base and the sliding components can perform the sliding movement within a range that includes an overlap position, where the sample receptacle portion overlaps with the electrode system, and the sample supply position.Type: ApplicationFiled: March 2, 2011Publication date: December 27, 2012Applicant: Nippon Kayaku Kabushiki KaishaInventors: Takao Yokoyama, Reiko Machida, Yayoi Irie, Yoshihiko Umegae
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Publication number: 20120094320Abstract: A protein comprising an amino acid sequence having at least one mutation selected from a Gly-4 to Ala mutation, a Glu-6 to His mutation, a Ser-14 to Thr mutation, an Ala-37 to Thr or Arg mutation, a Pro-50 to Gln mutation, a Glu-67 to Gly mutation, an Asp-80 to Tyr mutation, a Val-93 to Met mutation, an Arg-156 to Pro mutation, a Leu-164 to Met mutation, an Asn-202 to Asp mutation, a Thr-235 to Ala mutation, an Asn-348 to Tyr mutation, a Gly-362 to Arg mutation and a Val-473 to Ala mutation in the amino acid sequence depicted in SEQ II NO:4. (2) A thermostable protein which comprises an amino acid sequence derived from the amino acid sequence having at least one variation described in (1) and having 1,5-anhydroglucitol dehydrogenase activity. These proteins act specifically on 1,5-anhydroglucitol (1,5-AG), have thermal stability and exhibit excellent storage stability.Type: ApplicationFiled: June 18, 2009Publication date: April 19, 2012Applicant: NIPPON KAYAKU KABUSHIKI KAISHAInventors: Hideki Yoshioka, Shuhei Tsukamoto, Minoru Masuda, Reiko Machida, Yoshihiko Umegae, Masahiko Yabuuchi
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Publication number: 20110259762Abstract: The biosensor has an electrode system for electrochemically measuring 1,5-anhydroglucitol (1,5-AG) and a reagent layer formed on said electrode system. The reagent layer contains an enzyme for measuring 1,5-anhydroglucitol, phenothiazine compounds, a stabilizer selected from a group of compounds comprising metal salts, organic acids, and amino acids, and an acidic polymer compound as an optional ingredient. The biosensor has excellent storage stability and can electrochemically measure 1,5-anhydroglucitol unaffected by the hematocrit contained in a whole blood sample.Type: ApplicationFiled: December 7, 2009Publication date: October 27, 2011Applicant: NIPPON KAYAKU KABUSHIKI KAISHAInventors: Takao Yokoyama, Hisako Takagi, Yayoi Irie, Reiko Machida, Yoshihiko Umegae
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Publication number: 20110165608Abstract: The invention is a method for determining the concentration of an analyte in whole blood, wherein the analyte is a component which is contained in the blood, is different from a component occurring only in a red blood cell, and can generate hydrogen peroxide upon the reaction with an oxidase. Whole blood is utilized in the method. The method comprises the steps of hemolyzing the whole blood and detecting hydrogen peroxide generated by the reaction between the analyte and the oxidase. The measurement method can avoid the inhibition of color development by hemoglobin and the interference with the measurement by hemoglobin. Further it can be used for biological tests that are carried out in a household, an individual doctor's clinic or at the bedside of patients without the need for any blood cell separation procedure or the like, because the measurement utilizes whole blood.Type: ApplicationFiled: July 22, 2009Publication date: July 7, 2011Applicant: Nippon Kayaku Kabushiki KaishaInventors: Reiko Machida, Yayoi Irie, Masahiko Yabuuchi, Yoshihiko Umegae
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Publication number: 20110031118Abstract: A biosensor for electrochemically measuring a sample may include a first member made of an insulating material, an electrode system including a working electrode and a counter electrode formed on the first member, a second member fixed over the first member, a sample flow channel provided between the first member and the second member, a hydrophilic section provided on at least a part of the internal surface of the sample flow channel and extending from a first end near the electrode system to a second end on the opposite side, and a flow stop area provided on a section adjacent to the second end on the first member or on the internal surface of the sample flow channel.Type: ApplicationFiled: November 5, 2008Publication date: February 10, 2011Applicant: NIPPON KAYAKU KABUSHIKI KAISHAInventors: Reiko Machida, Takao Yokoyama, Yayoi Irie, Hisako Takagi, Yoshihiko Umegae, Toshio Tanabe
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Publication number: 20100062469Abstract: By a method for measuring 1,5-anhydroglucitol, comprising the steps of: eliminating or converting glucose interfering with the measurement of 1,5-anhydroglucitol and/or a derivative thereof beforehand; and measuring 1,5-anhydroglucitol performed thereafter, wherein such glucose and/or a derivative thereof are/is eliminated or converted in whole blood as such without performing blood cell separation, an enzyme for measuring 1,5-anhydroglucitol is allowed to act on without performing blood cell separation, and 1,5-anhydroglucitol is electrochemically measured, it becomes possible to measure 1,5-anhydroglucitol using a small amount of whole blood without resort to a centrifuge or the like. Accordingly, this measurement method can be applied to rapid measurement of 1,5-anhydroglucitol at bedside or in a medical examination room or to home self-measurement thereof by a patient.Type: ApplicationFiled: December 13, 2007Publication date: March 11, 2010Inventors: Yoshihiko Umegae, Reiko Machida, Hisako Takagi, Yayoi Irie, Takao Yokoyama, Toshio Tanabe
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Patent number: 5738997Abstract: Method of assay of enzymatic activity, comprising projecting excitation light to a sample containing an enzyme, a substrate which forms a product by action of the enzyme, and a reference substance which is insensitive to the action of the enzyme but emits fluorescence; obtaining a first measured value of fluorescence intensity of the sample at a first wavelength region which includes fluorescence emitted by the substrate or the product at least, obtaining a second measured value of fluorescence intensity at a second wavelength region which is different from the first wavelength region for the first measured value and includes fluorescence emitted by the reference substance; and assaying the enzymatic activity from the ratio of the first measured value to the second measured value and apparatus for performing the method. The method assures high accuracy and high sensitivity of measurement in enzyme labeled immunoassay and enzyme labeled DNA hybridization.Type: GrantFiled: June 6, 1995Date of Patent: April 14, 1998Assignee: Tosoh CorporationInventors: Hidechika Hayashi, Yoshihiko Umegae, Yukio Mitsuhisa
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Patent number: 5677134Abstract: Method of assay of enzymatic activity, comprising projecting excitation light to a sample containing an enzyme, a substrate which forms a product by action of the enzyme, and a reference substance which is insensitive to the action of the enzyme but emits fluorescence; obtaining a first measured value of fluorescence intensity of the sample at a first wavelength region which includes fluorescence emitted by the substrate or the product at least, obtaining a second measured value of fluorescence intensity at a second wavelength region which is different from the first wavelength region for the first measured value and includes fluorescence emitted by the reference substance; and assaying the enzymatic activity from the ratio of the first measured value to the second measured value and apparatus for performing the method. The method assures high accuracy and high sensitivity of measurement in enzyme labeled immunoassay and enzyme labeled DNA hybridization.Type: GrantFiled: April 3, 1995Date of Patent: October 14, 1997Assignee: Tosoh CorporationInventors: Hidechika Hayashi, Yoshihiko Umegae, Yukio Mitsuhisa
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Patent number: 5460943Abstract: Method of assay of enzymatic activity, including projecting excitation light to a sample containing an enzyme, a substrate which forms a product by action of the enzyme, and a reference substance which is insensitive to the action of the enzyme but emits fluorescence; obtaining a first measured value of fluorescence intensity of the sample at a first wavelength region which includes fluorescence emitted by the substrate or the product at least, obtaining a second measured value of fluorescence intensity at a second wavelength region which is different from the first wavelength region for the first measured value and includes fluorescence emitted by the reference substance; and assaying the enzymatic activity from the ratio of the first measured value to the second measured value and apparatus for performing the method. The method assures high accuracy and high sensitivity of measurement in enzyme labeled immunoassay and enzyme labeled DNA hybridization.Type: GrantFiled: June 18, 1992Date of Patent: October 24, 1995Assignee: Tosoh CorporationInventors: Hidechika Hayashi, Yoshihiko Umegae, Yukio Mitsuhisa