Patents by Inventor Yoshihiro Ashihara
Yoshihiro Ashihara has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 8252531Abstract: The present invention relates to an inhibitor capable of increasing accuracy of the reaction in hybridization, and inhibiting nonspecific hybridization, a clinical diagnostic reagent, and a method of clinical analysis whereby nonspecific hybridization in clinical analysis is inhibited, and a target nucleic substance is detected easily with high accuracy. The inhibitor and the clinical diagnostic reagent contains polymer (H) having a nonspecific hybridization inhibitory action. The polymer has in its molecule at least one of carboxyl and sulfone groups, and phosphorylcholine-like groups, and has a weight average molecular weight of 1000 to 5000000. The method of clinical analysis includes contacting a sample with a test agent capable of hybridizing with a specific nucleic substance in the presence of the inhibitor.Type: GrantFiled: April 25, 2002Date of Patent: August 28, 2012Assignee: NOF CorporationInventors: Shujiro Sakaki, Kenshiro Shuto, Mamoru Tsuchida, Yoshihiro Ashihara, Mitsuo Isomura
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Patent number: 7771928Abstract: Disclosed are an immunoassay device which comprises a labeled substance dotting portion and a specimen dotting portion provided thereon, and an immunoassay method using the device.Type: GrantFiled: August 28, 2002Date of Patent: August 10, 2010Assignee: Fujirebio Inc.Inventors: Akira Hasegawa, Yoshihiro Ashihara, Mitsuo Isomura
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Patent number: 7276343Abstract: The present invention provides a chemiluminescence enhancer treated to retain favorable dispersibility of fine solid carriers and stably exert a chemiluminescence enhancing action. The invention provides a chemiluminescence enhancer used for signal detection in a solid phase immunoassay using antigen or/and antibody immobilized onto fine solid carriers dispersible in a liquid medium, consisting of a water soluble macromolecular quaternary ammonium salt, a quaternary sulfonium salt or a quaternary phosphonium salt in order to enhance emission of light caused by an enzymatic reaction of a chemiluminescent substrate having dioxetane, wherein the chemiluminescence enhancer is given an aggregation inhibition treatment of the fine solid carriers by the treatment with an oxidizing agent or a reducing agent, and a chemiluminescence method and a kit using the chemiluminescence enhancer.Type: GrantFiled: June 24, 2003Date of Patent: October 2, 2007Assignee: Fujirebio Inc.Inventors: Kazushige Moriyama, Tetsuji Tanimoto, Tatsuki Matsuno, Yoshihiro Ashihara
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Publication number: 20070059786Abstract: The present invention provides a chemiluminescence enhancer treated to retain favorable dispersibility of fine solid carriers and stably exert a chemiluminescence enhancing action. The invention provides a chemiluminescence enhancer used for signal detection in a solid phase immunoassay using antigen or/and antibody immobilized onto fine solid carriers dispersible in a liquid medium, consisting of a water soluble macromolecular quaternary ammonium salt, a quaternary sulfonium salt or a quaternary phosphonium salt in order to enhance emission of light caused by an enzymatic reaction of a chemiluminescent substrate having dioxetane, wherein the chemiluminescence enhancer is given an aggregation inhibition treatment of the fine solid carriers by the treatment with an oxidizing agent or a reducing agent, and a chemiluminescence method and a kit using the chemiluminescence enhancer.Type: ApplicationFiled: June 7, 2006Publication date: March 15, 2007Applicant: FUJIREBIO INC.Inventors: Kazushige Moriyama, Tetsuji Tanimoto, Tatsuki Matsuno, Yoshihiro Ashihara
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Publication number: 20050239149Abstract: The present invention provides a chemiluminescence enhancer treated to retain favorable dispersibility of fine solid carriers and stably exert a chemiluminescence enhancing action. The invention provides a chemiluminescence enhancer used for signal detection in a solid phase immunoassay using antigen or/and antibody immobilized onto fine solid carriers dispersible in a liquid medium, consisting of a water soluble macromolecular quaternary ammonium salt, a quaternary sulfonium salt or a quaternary phosphonium salt in order to enhance emission of light caused by an enzymatic reaction of a chemiluminescent substrate having dioxetane, wherein the chemiluminescence enhancer is given an aggregation inhibition treatment of the fine solid carriers by the treatment with an oxidizing agent or a reducing agent, and a chemiluminescence method and a kit using the chemiluminescence enhancer.Type: ApplicationFiled: June 24, 2003Publication date: October 27, 2005Applicant: FUJIREBIO INC.Inventors: Kazushige Moriyama, Tetsuji Tanimoto, Tatsuki Matsuno, Yoshihiro Ashihara
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Publication number: 20040219546Abstract: The present invention relates to an inhibitor capable of increasing accuracy of the reaction in hybridization, and inhibiting nonspecific hybridization, a clinical diagnostic reagent, and a method of clinical analysis whereby nonspecific hybridization in clinical analysis is inhibited, and a target nucleic substance is detected easily with high accuracy. The inhibitor and the clinical diagnostic reagent contains polymer (H) having a nonspecific hybridization inhibitory action. The polymer has in its molecule at least one of carboxyl and sulfone groups, and phosphorylcholine-like groups, and has a weight average molecular weight of 1000 to 5000000. The method of clinical analysis includes contacting a sample with a test agent capable of hybridizing with a specific nucleic substance in the presence of the inhibitor.Type: ApplicationFiled: February 11, 2004Publication date: November 4, 2004Inventors: Shujiro Sakaki, Kenshiro Shuto, Mamoru Tsuchida, Yoshihiro Ashihara, Mitsuo Isomura
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Patent number: 6589754Abstract: An immunoassay element for quantitatively analyzing a macromolecular antigen by determining the change in enzymatic activity caused by a reaction between the macromolecular polymer antigen and an enzyme-labelled antibody. The immunoassay element comprises a substrate layer containing a non-diffusible substrate which forms a diffusible material in the presence of the enzyme, and a reagent layer containing a fragmenting enzyme for further fragmenting the diffusible material into a lower molecular weight product. Also provided is a process for quantitatively analyzing a macromolecular antigen in a sample by the use of the immunoassay element.Type: GrantFiled: October 8, 1997Date of Patent: July 8, 2003Assignees: Fuji Photo Film Co., Ltd., Fujirebio Inc.Inventors: Toshikage Hiraoka, Tetsuji Tanimoto, Yoshihiko Makino, Tadashi Ninomiya, Hiroshi Shinoki, Yoshihiro Ashihara, Naofumi Hora, Masashi Ogawa
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Publication number: 20030100103Abstract: Disclosed are an immunoassay device which comprises a labeled substance dotting portion and a specimen dotting portion provided thereon, and an immunoassay method using the device.Type: ApplicationFiled: August 28, 2002Publication date: May 29, 2003Applicant: FUJIREBIO INC.Inventors: Hiroko Saruta, Akira Hasegawa, Yoshihiro Ashihara, Yuko Ishioka, Mitsuo Isomura
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Patent number: 6472160Abstract: Disclosed are an immunoassay device which comprises a labeled substance dotting portion and a specimen dotting portion provided thereon, and an immunoassay method using the device.Type: GrantFiled: January 27, 2000Date of Patent: October 29, 2002Assignee: Fujirebio Inc.Inventors: Hiroko Saruta, Akira Hasegawa, Yoshihiro Ashihara, Yuko Ishioka, Mitsuo Isomura
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Publication number: 20020045278Abstract: Disclosed are an immunoassay device which comprises a labeled substance dotting portion and a specimen dotting portion provided thereon, and an immunoassay method using the device.Type: ApplicationFiled: January 27, 2000Publication date: April 18, 2002Applicant: Fujirebio INC.(Fujirebio Kabushiki Kaisha)Inventors: Hiroko Saruta, Akira Hasegawa, Yoshihiro Ashihara, Yuko Ishioka, Mitsuo Isomura
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Publication number: 20010005585Abstract: An enzyme-labeled immunoassay is performed by the steps of allowing a test sample to react with an enzyme-labeled reagent, allowing a substrate to react with the enzyme to form a signal, and immobilising the enzyme-labeled reagent, with the prevention of a further signal formation from a predetermined time on after the immobilisation of the enzyme-labeled reagent, using an enzyme inhibitor.Type: ApplicationFiled: February 8, 2001Publication date: June 28, 2001Applicant: FUJIREBIO INC.Inventors: Yoshihiro Ashihara, Mitsuo Isomura, Atsuka Sato
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Patent number: 6251618Abstract: Disclosed are a color developing method utilizing the reaction of an indolyl derivative with an enzyme in the presence of a specific free radical compound and/or a specific chelate compound, and an enzyme immunoassay using the color developing method.Type: GrantFiled: October 13, 1999Date of Patent: June 26, 2001Assignee: Fujirebio Inc.Inventors: Masami Sugiyama, Mamoru Miyazaki, Yoshihiro Ashihara
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Patent number: 6221625Abstract: An enzyme-labeled immunoassay is performed by the steps of allowing a test sample to react with an enzyme-labeled reagent, allowing a substrate to react with the enzyme to form a signal, and immobilising the enzyme-labeled reagent, with the prevention of a further signal formation from a predetermined time on after the immobilisation of the enzyme-labeled reagent, using an enzyme inhibitor.Type: GrantFiled: February 2, 1998Date of Patent: April 24, 2001Assignee: Fujirebio Inc.Inventors: Yoshihiro Ashihara, Mitsuo Isomura, Atsuka Sato
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Patent number: 6017494Abstract: A liquid supply device for transporting a liquid to a predetermined portion includes a liquid container for holding a liquid therein, which has an opening, a sealing sheet member which is impermeable but rupturable and seals the opening of the container, and an absorbent material which is capable of transporting the liquid by capillary action, the absorbent material being disposed on the sealing sheet member, out of contact with the liquid when the sealing sheet member is not ruptured, while when the sealing sheet member is ruptured and the absorbent material is brought into contact with the liquid, part of the absorbent material being continuously held within the liquid and transporting the liquid to the predetermined position. An analytical test device using the above liquid supply device is provided, which may be used, for instance, immunoassay.Type: GrantFiled: April 24, 1998Date of Patent: January 25, 2000Assignee: Fujirebio Inc.Inventors: Yoshihiro Ashihara, Mitsushi Gotanda, Akira Hasegawa, Yuko Fujiwara
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Patent number: 5998156Abstract: Disclosed are a color developing method utilizing the reaction of an indolyl derivative with an enzyme in the presence of a specific free radical compound and/or a specific chelate compound, and an enzyme immunoassay using the color developing method.Type: GrantFiled: June 25, 1998Date of Patent: December 7, 1999Assignee: Fujirebio Inc.Inventors: Masami Sugiyama, Mamoru Miyazaki, Yoshihiro Ashihara
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Patent number: 5736349Abstract: There are disclosed a magnetic particle for an immunoassay method, which comprises a core and a coating layer formed on the surface of the core wherein said core comprises an organic polymer matrix and said coating layer comprises a mixed crystal ferrite represented by the formula:M.sub.x Fe.sub.(3-x) O.sub.4wherein M represents at least one metal selected from the group consisting of Mn, Ni, Zn, Co, Cu, Mg, Sn, Ca and Cd, and x is a number satisfying the relation: 0<x<3,and an antigen or an antibody is bound onto the surface of the coating layer and wherein said particle has a particle size of 0.03 to 10 .mu.m, and an immunoassay method using the same.Type: GrantFiled: May 12, 1995Date of Patent: April 7, 1998Assignees: Nippon Paint Co., Ltd., Fujirebio Inc. (Fujirebio Kabushiki Kaisha)Inventors: Motohiro Sasaki, Masahiko Matsukawa, Katsuaki Yoshioka, Makoto Anan, Mitsuo Isomura, Yoshihiro Ashihara, Masahisa Okada
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Patent number: 5603898Abstract: A dry-type analytical element for immunoassay having at least one water-permeable layer for measuring a ligand in a sample according to enzyme immunoassay, which comprises,(A) a water-soluble macromolecular substrate, and(B) an enzyme conjugated with antibody reacting with the ligand in the sample capable to act on the above water-soluble macromolecular substrate. The above analytical element further comprises,(C) a macromolecular antigen which is a conjugate of the ligand or its derivative with a macromolecular compound in the above water-permeable layer of the above dry-type analytical element.The invention can conduct highly sensitive and simple enzyme immunoassay.Type: GrantFiled: December 17, 1992Date of Patent: February 18, 1997Assignees: Fujirejiro Inc., Fuji Photo Film Co., Ltd.Inventors: Yoshihiro Ashihara, Yukio Sudo, Isao Nishizono, Toshikage Hiraoka, Tetsuji Tanimoto, Shigeki Kageyama
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Patent number: 5569589Abstract: An immunoassay element for quantitatively analyzing a ligand by determining the change in enzymatic activity caused by a reaction between the ligand, a linked product of the ligand and a high molecular weight compound and an enzyme-labelled antibody. The immunoassay element comprises a substrate layer containing a non-diffusible substrate which forms a diffusible material in the presence of the enzyme, and a reagent layer containing a fragmenting enzyme for further fragmenting the non-difussible material. Also provided is a process for quantitatively analyzing a ligand contained in a sample by the use of the immunoassay element.Type: GrantFiled: December 7, 1993Date of Patent: October 29, 1996Assignees: Fuji Photo Film Co., Ltd., Fujirebio Inc.Inventors: Toshikage Hiraoka, Tetsuji Tanimoto, Yoshihiko Makino, Tadashi Ninomiya, Naofumi Hora, Yoshihiro Ashihara, Yukio Sudo, Toshihiro Mori
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Patent number: 5482839Abstract: An automatic immunoassay apparatus utilizes cartridges each having at least two wells, a first well of said wells containing solid phase material carrying antigen or antibody, a second well of said wells containing antibody or antigen labelled with labelling compound. The wells may be sealed with a suitable sealing film before use and the sealing film is broken when the cartridge is to be used. The cartridges are transported to a predetermined position on a steppingly movable reaction line and conveyed thereby at a predetermined interval. While the reaction line steps, a sample, labelled antigen or antibody contained in the second well and substrate, if necessary, are added to the first well and stirred at predetermined timings and reaction between the sample and a reactive solution is measured under control of a control device having memory means storing operator selectable programs for various measuring methods.Type: GrantFiled: May 4, 1993Date of Patent: January 9, 1996Inventors: Yoshihiro Ashihara, Isao Nishizono, Hidetaka Minakawa, Masahisa Okada, Yasusuke Sakurabayashi, Fumio Watanabe, Shin-ichi Wakana
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Patent number: 5320944Abstract: An immunoassay method using magnetic particles comprising a core and a coating layer on the surface thereof, The core comprises an organic polymer and the coating layer comprises an iron oxide type ferrite coating layer. An antigen or an antibody is bound onto the surface of the coating layer and the particle has a particle size of 0.2 to 3 .mu.m.Type: GrantFiled: October 22, 1992Date of Patent: June 14, 1994Assignees: Fujirebio Inc., Nippon Paint Co., Ltd.Inventors: Masahisa Okada, Yoshihiro Ashihara, Akira Yano, Masao Oishi, Katsuaki Yoshioka, Toshiomi Nakamura