Patents by Inventor Yoshihiro Ashihara

Yoshihiro Ashihara has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 8252531
    Abstract: The present invention relates to an inhibitor capable of increasing accuracy of the reaction in hybridization, and inhibiting nonspecific hybridization, a clinical diagnostic reagent, and a method of clinical analysis whereby nonspecific hybridization in clinical analysis is inhibited, and a target nucleic substance is detected easily with high accuracy. The inhibitor and the clinical diagnostic reagent contains polymer (H) having a nonspecific hybridization inhibitory action. The polymer has in its molecule at least one of carboxyl and sulfone groups, and phosphorylcholine-like groups, and has a weight average molecular weight of 1000 to 5000000. The method of clinical analysis includes contacting a sample with a test agent capable of hybridizing with a specific nucleic substance in the presence of the inhibitor.
    Type: Grant
    Filed: April 25, 2002
    Date of Patent: August 28, 2012
    Assignee: NOF Corporation
    Inventors: Shujiro Sakaki, Kenshiro Shuto, Mamoru Tsuchida, Yoshihiro Ashihara, Mitsuo Isomura
  • Patent number: 7771928
    Abstract: Disclosed are an immunoassay device which comprises a labeled substance dotting portion and a specimen dotting portion provided thereon, and an immunoassay method using the device.
    Type: Grant
    Filed: August 28, 2002
    Date of Patent: August 10, 2010
    Assignee: Fujirebio Inc.
    Inventors: Akira Hasegawa, Yoshihiro Ashihara, Mitsuo Isomura
  • Patent number: 7276343
    Abstract: The present invention provides a chemiluminescence enhancer treated to retain favorable dispersibility of fine solid carriers and stably exert a chemiluminescence enhancing action. The invention provides a chemiluminescence enhancer used for signal detection in a solid phase immunoassay using antigen or/and antibody immobilized onto fine solid carriers dispersible in a liquid medium, consisting of a water soluble macromolecular quaternary ammonium salt, a quaternary sulfonium salt or a quaternary phosphonium salt in order to enhance emission of light caused by an enzymatic reaction of a chemiluminescent substrate having dioxetane, wherein the chemiluminescence enhancer is given an aggregation inhibition treatment of the fine solid carriers by the treatment with an oxidizing agent or a reducing agent, and a chemiluminescence method and a kit using the chemiluminescence enhancer.
    Type: Grant
    Filed: June 24, 2003
    Date of Patent: October 2, 2007
    Assignee: Fujirebio Inc.
    Inventors: Kazushige Moriyama, Tetsuji Tanimoto, Tatsuki Matsuno, Yoshihiro Ashihara
  • Publication number: 20070059786
    Abstract: The present invention provides a chemiluminescence enhancer treated to retain favorable dispersibility of fine solid carriers and stably exert a chemiluminescence enhancing action. The invention provides a chemiluminescence enhancer used for signal detection in a solid phase immunoassay using antigen or/and antibody immobilized onto fine solid carriers dispersible in a liquid medium, consisting of a water soluble macromolecular quaternary ammonium salt, a quaternary sulfonium salt or a quaternary phosphonium salt in order to enhance emission of light caused by an enzymatic reaction of a chemiluminescent substrate having dioxetane, wherein the chemiluminescence enhancer is given an aggregation inhibition treatment of the fine solid carriers by the treatment with an oxidizing agent or a reducing agent, and a chemiluminescence method and a kit using the chemiluminescence enhancer.
    Type: Application
    Filed: June 7, 2006
    Publication date: March 15, 2007
    Applicant: FUJIREBIO INC.
    Inventors: Kazushige Moriyama, Tetsuji Tanimoto, Tatsuki Matsuno, Yoshihiro Ashihara
  • Publication number: 20050239149
    Abstract: The present invention provides a chemiluminescence enhancer treated to retain favorable dispersibility of fine solid carriers and stably exert a chemiluminescence enhancing action. The invention provides a chemiluminescence enhancer used for signal detection in a solid phase immunoassay using antigen or/and antibody immobilized onto fine solid carriers dispersible in a liquid medium, consisting of a water soluble macromolecular quaternary ammonium salt, a quaternary sulfonium salt or a quaternary phosphonium salt in order to enhance emission of light caused by an enzymatic reaction of a chemiluminescent substrate having dioxetane, wherein the chemiluminescence enhancer is given an aggregation inhibition treatment of the fine solid carriers by the treatment with an oxidizing agent or a reducing agent, and a chemiluminescence method and a kit using the chemiluminescence enhancer.
    Type: Application
    Filed: June 24, 2003
    Publication date: October 27, 2005
    Applicant: FUJIREBIO INC.
    Inventors: Kazushige Moriyama, Tetsuji Tanimoto, Tatsuki Matsuno, Yoshihiro Ashihara
  • Publication number: 20040219546
    Abstract: The present invention relates to an inhibitor capable of increasing accuracy of the reaction in hybridization, and inhibiting nonspecific hybridization, a clinical diagnostic reagent, and a method of clinical analysis whereby nonspecific hybridization in clinical analysis is inhibited, and a target nucleic substance is detected easily with high accuracy. The inhibitor and the clinical diagnostic reagent contains polymer (H) having a nonspecific hybridization inhibitory action. The polymer has in its molecule at least one of carboxyl and sulfone groups, and phosphorylcholine-like groups, and has a weight average molecular weight of 1000 to 5000000. The method of clinical analysis includes contacting a sample with a test agent capable of hybridizing with a specific nucleic substance in the presence of the inhibitor.
    Type: Application
    Filed: February 11, 2004
    Publication date: November 4, 2004
    Inventors: Shujiro Sakaki, Kenshiro Shuto, Mamoru Tsuchida, Yoshihiro Ashihara, Mitsuo Isomura
  • Patent number: 6589754
    Abstract: An immunoassay element for quantitatively analyzing a macromolecular antigen by determining the change in enzymatic activity caused by a reaction between the macromolecular polymer antigen and an enzyme-labelled antibody. The immunoassay element comprises a substrate layer containing a non-diffusible substrate which forms a diffusible material in the presence of the enzyme, and a reagent layer containing a fragmenting enzyme for further fragmenting the diffusible material into a lower molecular weight product. Also provided is a process for quantitatively analyzing a macromolecular antigen in a sample by the use of the immunoassay element.
    Type: Grant
    Filed: October 8, 1997
    Date of Patent: July 8, 2003
    Assignees: Fuji Photo Film Co., Ltd., Fujirebio Inc.
    Inventors: Toshikage Hiraoka, Tetsuji Tanimoto, Yoshihiko Makino, Tadashi Ninomiya, Hiroshi Shinoki, Yoshihiro Ashihara, Naofumi Hora, Masashi Ogawa
  • Publication number: 20030100103
    Abstract: Disclosed are an immunoassay device which comprises a labeled substance dotting portion and a specimen dotting portion provided thereon, and an immunoassay method using the device.
    Type: Application
    Filed: August 28, 2002
    Publication date: May 29, 2003
    Applicant: FUJIREBIO INC.
    Inventors: Hiroko Saruta, Akira Hasegawa, Yoshihiro Ashihara, Yuko Ishioka, Mitsuo Isomura
  • Patent number: 6472160
    Abstract: Disclosed are an immunoassay device which comprises a labeled substance dotting portion and a specimen dotting portion provided thereon, and an immunoassay method using the device.
    Type: Grant
    Filed: January 27, 2000
    Date of Patent: October 29, 2002
    Assignee: Fujirebio Inc.
    Inventors: Hiroko Saruta, Akira Hasegawa, Yoshihiro Ashihara, Yuko Ishioka, Mitsuo Isomura
  • Publication number: 20020045278
    Abstract: Disclosed are an immunoassay device which comprises a labeled substance dotting portion and a specimen dotting portion provided thereon, and an immunoassay method using the device.
    Type: Application
    Filed: January 27, 2000
    Publication date: April 18, 2002
    Applicant: Fujirebio INC.(Fujirebio Kabushiki Kaisha)
    Inventors: Hiroko Saruta, Akira Hasegawa, Yoshihiro Ashihara, Yuko Ishioka, Mitsuo Isomura
  • Publication number: 20010005585
    Abstract: An enzyme-labeled immunoassay is performed by the steps of allowing a test sample to react with an enzyme-labeled reagent, allowing a substrate to react with the enzyme to form a signal, and immobilising the enzyme-labeled reagent, with the prevention of a further signal formation from a predetermined time on after the immobilisation of the enzyme-labeled reagent, using an enzyme inhibitor.
    Type: Application
    Filed: February 8, 2001
    Publication date: June 28, 2001
    Applicant: FUJIREBIO INC.
    Inventors: Yoshihiro Ashihara, Mitsuo Isomura, Atsuka Sato
  • Patent number: 6251618
    Abstract: Disclosed are a color developing method utilizing the reaction of an indolyl derivative with an enzyme in the presence of a specific free radical compound and/or a specific chelate compound, and an enzyme immunoassay using the color developing method.
    Type: Grant
    Filed: October 13, 1999
    Date of Patent: June 26, 2001
    Assignee: Fujirebio Inc.
    Inventors: Masami Sugiyama, Mamoru Miyazaki, Yoshihiro Ashihara
  • Patent number: 6221625
    Abstract: An enzyme-labeled immunoassay is performed by the steps of allowing a test sample to react with an enzyme-labeled reagent, allowing a substrate to react with the enzyme to form a signal, and immobilising the enzyme-labeled reagent, with the prevention of a further signal formation from a predetermined time on after the immobilisation of the enzyme-labeled reagent, using an enzyme inhibitor.
    Type: Grant
    Filed: February 2, 1998
    Date of Patent: April 24, 2001
    Assignee: Fujirebio Inc.
    Inventors: Yoshihiro Ashihara, Mitsuo Isomura, Atsuka Sato
  • Patent number: 6017494
    Abstract: A liquid supply device for transporting a liquid to a predetermined portion includes a liquid container for holding a liquid therein, which has an opening, a sealing sheet member which is impermeable but rupturable and seals the opening of the container, and an absorbent material which is capable of transporting the liquid by capillary action, the absorbent material being disposed on the sealing sheet member, out of contact with the liquid when the sealing sheet member is not ruptured, while when the sealing sheet member is ruptured and the absorbent material is brought into contact with the liquid, part of the absorbent material being continuously held within the liquid and transporting the liquid to the predetermined position. An analytical test device using the above liquid supply device is provided, which may be used, for instance, immunoassay.
    Type: Grant
    Filed: April 24, 1998
    Date of Patent: January 25, 2000
    Assignee: Fujirebio Inc.
    Inventors: Yoshihiro Ashihara, Mitsushi Gotanda, Akira Hasegawa, Yuko Fujiwara
  • Patent number: 5998156
    Abstract: Disclosed are a color developing method utilizing the reaction of an indolyl derivative with an enzyme in the presence of a specific free radical compound and/or a specific chelate compound, and an enzyme immunoassay using the color developing method.
    Type: Grant
    Filed: June 25, 1998
    Date of Patent: December 7, 1999
    Assignee: Fujirebio Inc.
    Inventors: Masami Sugiyama, Mamoru Miyazaki, Yoshihiro Ashihara
  • Patent number: 5736349
    Abstract: There are disclosed a magnetic particle for an immunoassay method, which comprises a core and a coating layer formed on the surface of the core wherein said core comprises an organic polymer matrix and said coating layer comprises a mixed crystal ferrite represented by the formula:M.sub.x Fe.sub.(3-x) O.sub.4wherein M represents at least one metal selected from the group consisting of Mn, Ni, Zn, Co, Cu, Mg, Sn, Ca and Cd, and x is a number satisfying the relation: 0<x<3,and an antigen or an antibody is bound onto the surface of the coating layer and wherein said particle has a particle size of 0.03 to 10 .mu.m, and an immunoassay method using the same.
    Type: Grant
    Filed: May 12, 1995
    Date of Patent: April 7, 1998
    Assignees: Nippon Paint Co., Ltd., Fujirebio Inc. (Fujirebio Kabushiki Kaisha)
    Inventors: Motohiro Sasaki, Masahiko Matsukawa, Katsuaki Yoshioka, Makoto Anan, Mitsuo Isomura, Yoshihiro Ashihara, Masahisa Okada
  • Patent number: 5603898
    Abstract: A dry-type analytical element for immunoassay having at least one water-permeable layer for measuring a ligand in a sample according to enzyme immunoassay, which comprises,(A) a water-soluble macromolecular substrate, and(B) an enzyme conjugated with antibody reacting with the ligand in the sample capable to act on the above water-soluble macromolecular substrate. The above analytical element further comprises,(C) a macromolecular antigen which is a conjugate of the ligand or its derivative with a macromolecular compound in the above water-permeable layer of the above dry-type analytical element.The invention can conduct highly sensitive and simple enzyme immunoassay.
    Type: Grant
    Filed: December 17, 1992
    Date of Patent: February 18, 1997
    Assignees: Fujirejiro Inc., Fuji Photo Film Co., Ltd.
    Inventors: Yoshihiro Ashihara, Yukio Sudo, Isao Nishizono, Toshikage Hiraoka, Tetsuji Tanimoto, Shigeki Kageyama
  • Patent number: 5569589
    Abstract: An immunoassay element for quantitatively analyzing a ligand by determining the change in enzymatic activity caused by a reaction between the ligand, a linked product of the ligand and a high molecular weight compound and an enzyme-labelled antibody. The immunoassay element comprises a substrate layer containing a non-diffusible substrate which forms a diffusible material in the presence of the enzyme, and a reagent layer containing a fragmenting enzyme for further fragmenting the non-difussible material. Also provided is a process for quantitatively analyzing a ligand contained in a sample by the use of the immunoassay element.
    Type: Grant
    Filed: December 7, 1993
    Date of Patent: October 29, 1996
    Assignees: Fuji Photo Film Co., Ltd., Fujirebio Inc.
    Inventors: Toshikage Hiraoka, Tetsuji Tanimoto, Yoshihiko Makino, Tadashi Ninomiya, Naofumi Hora, Yoshihiro Ashihara, Yukio Sudo, Toshihiro Mori
  • Patent number: 5482839
    Abstract: An automatic immunoassay apparatus utilizes cartridges each having at least two wells, a first well of said wells containing solid phase material carrying antigen or antibody, a second well of said wells containing antibody or antigen labelled with labelling compound. The wells may be sealed with a suitable sealing film before use and the sealing film is broken when the cartridge is to be used. The cartridges are transported to a predetermined position on a steppingly movable reaction line and conveyed thereby at a predetermined interval. While the reaction line steps, a sample, labelled antigen or antibody contained in the second well and substrate, if necessary, are added to the first well and stirred at predetermined timings and reaction between the sample and a reactive solution is measured under control of a control device having memory means storing operator selectable programs for various measuring methods.
    Type: Grant
    Filed: May 4, 1993
    Date of Patent: January 9, 1996
    Inventors: Yoshihiro Ashihara, Isao Nishizono, Hidetaka Minakawa, Masahisa Okada, Yasusuke Sakurabayashi, Fumio Watanabe, Shin-ichi Wakana
  • Patent number: 5320944
    Abstract: An immunoassay method using magnetic particles comprising a core and a coating layer on the surface thereof, The core comprises an organic polymer and the coating layer comprises an iron oxide type ferrite coating layer. An antigen or an antibody is bound onto the surface of the coating layer and the particle has a particle size of 0.2 to 3 .mu.m.
    Type: Grant
    Filed: October 22, 1992
    Date of Patent: June 14, 1994
    Assignees: Fujirebio Inc., Nippon Paint Co., Ltd.
    Inventors: Masahisa Okada, Yoshihiro Ashihara, Akira Yano, Masao Oishi, Katsuaki Yoshioka, Toshiomi Nakamura