Patents by Inventor Yoshihiro Ohmiya

Yoshihiro Ohmiya has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Nucleic acid encoding a luminescent enzyme protein
    Patent number: 11015176
    Abstract: The present invention provides a luminescent enzyme protein comprising the amino acid sequence represented by SEQ ID NO: 2, and a mutant enzyme thereof.
    Type: Grant
    Filed: March 9, 2017
    Date of Patent: May 25, 2021
    Assignee: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY
    Inventors: Yoshihiro Ohmiya, Yasuo Mitani, Rie Yasuno
  • LIGHT-EMITTING ENZYME PROTEIN
    Publication number: 20190093086
    Abstract: The present invention provides a luminescent enzyme protein comprising the amino acid sequence represented by SEQ ID NO: 2, and a mutant enzyme thereof.
    Type: Application
    Filed: March 9, 2017
    Publication date: March 28, 2019
    Applicant: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY
    Inventors: Yoshihiro OHMIYA, Yasuo MITANI, Rie YASUNO
  • Light emitting element, reference light source and method for observing luminous body
    Patent number: 10094709
    Abstract: A light-emitting element, in which a light whose emission angle distribution is one of Lambert's emission law or uniform Isotropic emission, is extracted from a light extraction opening window, and an in-plane distribution of a light intensity on a light extraction surface of the light extraction opening window is uniform, and which can be used as a reference light source when measuring an absolute light quantity of a weak light emitted from a luminous body which is a measurement object.
    Type: Grant
    Filed: December 22, 2014
    Date of Patent: October 9, 2018
    Assignees: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY, ATTO CORPORATION
    Inventors: Hidefumi Akiyama, Masahiro Yoshita, Yoshihiro Ohmiya, Hidehiro Kubota, Kaneo Mori, Masahiro Shimogawara
  • LIGHT EMITTING ELEMENT, REFERENCE LIGHT SOURCE AND METHOD FOR OBSERVING LUMINOUS BODY
    Publication number: 20170191872
    Abstract: A light-emitting element, in which a light whose emission angle distribution is one of Lambert's emission law or uniform Isotropic emission, is extracted from a light extraction opening window, and an in-plane distribution of a light intensity on a light extraction surface of the light extraction opening window is uniform, and which can be used as a reference light source when measuring an absolute light quantity of a weak light emitted from a luminous body which is a measurement object.
    Type: Application
    Filed: December 22, 2014
    Publication date: July 6, 2017
    Inventors: Hidefumi AKIYAMA, Masahiro YOSHITA, Yoshihiro OHMIYA, Hidehiro KUBOTA, Kaneo MORI, Masahiro SHIMOGAWARA
  • Method for producing complex of biotin-labeled () luciferase with streptoavidin and method for stabilizing the same
    Patent number: 8465938
    Abstract: The present invention relates to a method for producing Cypridina luciferase labeled with hydrophilic biotin, characterized in that a biotin reagent containing a polyalkylene glycol structure as a spacer is reacted with Cypridina luciferase, and biotin-labeled Cypridina luciferase wherein a sugar chain in Cypridina luciferase has been biotinylated.
    Type: Grant
    Filed: December 3, 2010
    Date of Patent: June 18, 2013
    Assignee: National Institute of Advanced Industrial Science and Technolgy
    Inventors: Yoshihiro Ohmiya, Chun Wu, Satoru Ohgiya, Kosei Kawasaki
  • Luciferase gene optimized for use in imaging of intracellular luminescence
    Patent number: 8383797
    Abstract: The present invention provides a gene construct encoding pH insensitive luciferase for visualizing intracellular information, wherein an intracellular expression activity is higher compared with a gene construct of luciferase derived from a firefly.
    Type: Grant
    Filed: November 13, 2006
    Date of Patent: February 26, 2013
    Assignees: Toyo Boseki Kabushiki Kaisha, National Institute of Advanced Industrial Science and Technology
    Inventors: Yoshihiro Ohmiya, Yoshihiro Nakajima, Vadim Viviani, Shigeaki Nishii, Tomomi Asai
  • Two secretory luciferases
    Patent number: 8367357
    Abstract: The present invention is one gene construct or a combination of two gene constructs or expression vectors incorporating a Cypridina luciferase gene and a copepod luciferase under the control of distinct promoters. These gene constructs and expression vectors are useful for making a mammalian cell incorporating the Cypridina luciferase gene and the copepod luciferase to be capable of stably expressed and extracellularly secreted under the control of the distinct promoters.
    Type: Grant
    Filed: October 23, 2009
    Date of Patent: February 5, 2013
    Assignee: National Institute of Advanced Industrial Science and Technology
    Inventors: Yoshihiro Ohmiya, Chun Wu
  • Luciferin luminescent substrate of marine ostracod crustacean and method for production thereof
    Patent number: 8343729
    Abstract: The present invention relates to a method for producing marine ostracod crustacean luciferin or a derivative thereof represented by a general formula (4), characterized by reacting a compound represented by a general formula (2) with a compound represented by a general formula (3): wherein R1, R2, R3, R5, Y1 and Z1 are the same as defined in the specification.
    Type: Grant
    Filed: June 9, 2011
    Date of Patent: January 1, 2013
    Assignee: National Institute of Advanced Industrial Science and Technology
    Inventors: Yoshihiro Ohmiya, Chun Wu
  • Mutant luciferase
    Patent number: 8147842
    Abstract: An object of the present invention is to provide a mutant luciferase having luciferase activity with an altered emission spectrum. A specific amino acid residue(s) is substituted in a luciferase derived from Cypridina noctiluca and then the resulting mutant luciferase having luciferase activity with an emission spectrum differing from that of the wild-type luciferase is screened for.
    Type: Grant
    Filed: January 26, 2007
    Date of Patent: April 3, 2012
    Assignee: National Institute of Advanced Industrial Science and Technology
    Inventors: Kosei Kawasaki, Yousuke Morita, Satoru Ohgiya, Yoshihiro Ohmiya, Yasushi Ohyama
  • METHOD FOR PRODUCING COMPLEX OF BIOTIN-LABELED CYPRIDINA (CYPRIDINA NOCTILUCA) LUCIFERASE WITH STREPTOAVIDIN AND METHOD FOR STABILIZING THE SAME
    Publication number: 20120077205
    Abstract: The present invention relates to a method for producing Cypridina luciferase labeled with hydrophilic biotin, characterized in that a biotin reagent containing a polyalkylene glycol structure as a spacer is reacted with Cypridina luciferase, and biotin-labeled Cypridina luciferase wherein a sugar chain in Cypridina luciferase has been biotinylated.
    Type: Application
    Filed: December 3, 2010
    Publication date: March 29, 2012
    Inventors: Yoshihiro Ohmiya, Chun Wu, Satoru Ohgiya, Kosei Kawasaki
  • Visible to near-infrared light probe using energy transfer between luciferase and an organic dye via a sugar chain
    Patent number: 8143013
    Abstract: An object of the present invention is to produce a luminescent probe that has less biological effects, efficiently emits visible to near-infrared light, which is excellent for the imaging of individuals, and the use thereof. The present invention provides a sugar chain-containing-luciferase derivative, wherein an organic fluorescent dye is bonded to the luciferase through the sugar chain.
    Type: Grant
    Filed: August 7, 2008
    Date of Patent: March 27, 2012
    Assignee: National Institute of Advanced Industrial Science and Technology
    Inventors: Yoshihiro Ohmiya, Chun Wu
  • Luciferin luminescent substrate of marine ostracod crustacean and method for production thereof
    Publication number: 20110287459
    Abstract: The present invention relates to a method for producing marine ostracod crustacean luciferin or a derivative thereof represented by a general formula (4), characterized by reacting a compound represented by a general formula (2) with a compound represented by a general formula (3): wherein R1, R2, R3, R5, Y1 and Z1 are the same as defined in the specification.
    Type: Application
    Filed: June 9, 2011
    Publication date: November 24, 2011
    Applicant: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY
    Inventors: Yoshihiro Ohmiya, Chun Wu
  • Two secretory luciferases
    Publication number: 20110223610
    Abstract: The present invention is one gene construct or a combination of two gene constructs or expression vectors incorporating a Cypridina luciferase gene and a copepod luciferase under the control of distinct promoters. These gene constructs and expression vectors are useful for making a mammalian cell incorporating the Cypridina luciferase gene and the copepod luciferase to be capable of stably expressed and extracellularly secreted under the control of the distinct promoters.
    Type: Application
    Filed: October 23, 2009
    Publication date: September 15, 2011
    Inventors: Yoshihiro Ohmiya, Chun Wu
  • Method for producing substituted imidazo[1,2-A]pyrazines of (s)-1-(3-(2-sec-butyl-6-(1h-indol-3-yl)-3-oxo-3,7-dihydroimadazo[1,2-a]pyrazin-8-yl)propyl)guanidine
    Patent number: 7989621
    Abstract: The present invention relates to a method for producing marine ostracod crustacean luciferin or a derivative thereof represented by a general formula (4), characterized by reacting a compound represented by a general formula (2) with a compound represented by a general formula (3): wherein R1, R2, R3, R5, Y1 and Z1 are the same as defined in the specification.
    Type: Grant
    Filed: September 25, 2006
    Date of Patent: August 2, 2011
    Assignee: National Institute of Advanced Industrial Science and Technology
    Inventors: Yoshihiro Ohmiya, Chun Wu
  • Stabilizing composition and stabilizing method of coelenterazine solution for high-throughput measurement of luciferase activity
    Patent number: 7718389
    Abstract: The present invention relates to a stabilization composition of coelenterazine or an analog thereof, a kit, a method for measuring a coelenterazine-based biological luminescence, containing coelenterazine or the analog thereof and an antioxidant.
    Type: Grant
    Filed: October 13, 2006
    Date of Patent: May 18, 2010
    Assignee: National Institute of Advanced Industrial Science and Technology
    Inventors: Yoshihiro Ohmiya, Chun Wu
  • Composition and a method for suppressing the luminescence intensity decline in a luciferin-luciferase reaction
    Publication number: 20100120075
    Abstract: The invention relates to compositions and methods for suppressing the luminescence intensity decline that occurs as the reaction between Cypridina luciferase and luciferin proceeds.
    Type: Application
    Filed: November 12, 2008
    Publication date: May 13, 2010
    Inventors: Yoshihiro Ohmiya, Chun Wu
  • LUCIFERASE GENE OPTIMIZED FOR USE IN IMAGING OF INTRACELLULAR LUMINESCENCE
    Publication number: 20100112553
    Abstract: The present invention provides a gene construct encoding pH insensitive luciferase for visualizing intracellular information, wherein an intracellular expression activity is higher compared with a gene construct of luciferase derived from a firefly.
    Type: Application
    Filed: November 13, 2006
    Publication date: May 6, 2010
    Applicants: Toyo Boseki Kabushiki Kaisha, National Institute of Advanced Industrial Science and Technology
    Inventors: Yoshihiro OHMIYA, Yoshihiro NAKAJIMA, Vadim VIVIANI, Shigeaki NISHII, Tomomi ASAI
  • MUTANT LUCIFERASE
    Publication number: 20090263880
    Abstract: An object of the present invention is to provide a mutant luciferase having luciferase activity with an altered emission spectrum. A specific amino acid residue(s) is substituted in a luciferase derived from Cypridina noctiluca and then the resulting mutant luciferase having luciferase activity with an emission spectrum differing from that of the wild-type luciferase is screened for.
    Type: Application
    Filed: January 26, 2007
    Publication date: October 22, 2009
    Applicant: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY
    Inventors: Kosei Kawasaki, Yousuke Morita, Satoru Ohgiya, Yoshihiro Ohmiya, Yasushi Ohyama
  • LUCIFERASE GENE OPTIMIZED FOR USE IN IMAGING OF INTRACELLULAR LUMINESCENCE
    Publication number: 20090220960
    Abstract: The present invention provides a gene construct encoding pH insensitive luciferase for visualizing intracellular information, wherein an intracellular expression activity is higher compared with a gene construct of luciferase derived from a firefly.
    Type: Application
    Filed: November 13, 2006
    Publication date: September 3, 2009
    Applicant: TOYO BOSEKI KABUSHIKI KAISHA
    Inventors: Yoshihiro Ohmiya, Yoshihiro Nakajima, Vadim Viviani, Shigeaki Nishii, Tomomi Asai
  • Multiple gene transcription activity assay system
    Patent number: 7572629
    Abstract: A gene construct incorporating any of at least two luciferase genes which emit lights with different colors using an identical substrate such that the gene can be stably expressed in mammalian cells.
    Type: Grant
    Filed: April 30, 2004
    Date of Patent: August 11, 2009
    Assignee: National Institute of Advanced Industrial Science and Technology
    Inventors: Yoshihiro Ohmiya, Yoshihiro Nakajima