Patents by Inventor Yoshihiro Shimada

Yoshihiro Shimada has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 10996454
    Abstract: A microscope includes a movable stage supporting wells arranged in an array, a first imaging unit having a low-magnification objective lens, a second imaging unit having a high-magnification objective lens, a computer determining a representative position of a spheroid based on imaging data of the spheroid acquired by the first imaging unit, and a controller causing respective imaging units to sequentially acquire imaging data of the spheroid in each of the wells. The controller causes the first imaging unit to acquire imaging data for the spheroid in one of the wells, and then causes the stage to adjust the representative position to the optical axis of the high-magnification objective lens, and further causes the second imaging unit to acquire imaging data while causing the first imaging unit to acquire imaging data of the spheroid in another of the wells in synchronization with acquisition by the second imaging unit.
    Type: Grant
    Filed: March 29, 2019
    Date of Patent: May 4, 2021
    Assignee: OLYMPUS CORPORATION
    Inventor: Yoshihiro Shimada
  • Patent number: 10895728
    Abstract: Provided is a sample observation method including: bringing a gel-like transparent sample that encloses an observation target into contact with a transparent flat surface section of a substrate; and collecting light from the observation target by means of an objective lens via the substrate, in a state in which a pressing force is made to act in a direction in which the sample and the flat surface section relatively approach each other, until the contact area between the sample and the flat surface section comes to have a size allowing an effective light flux for the objective lens of a microscope to pass therethrough.
    Type: Grant
    Filed: November 9, 2018
    Date of Patent: January 19, 2021
    Assignee: OLYMPUS CORPORATION
    Inventors: Yoshihiro Shimada, Kazuhito Goda
  • Patent number: 10859807
    Abstract: An inverted microscope includes a stage supporting a bottom member; an immersion objective disposed facing vertically upward such that a distal end thereof faces a bottom face of the bottom member; an alignment unit moving the stage or the immersion objective in an optical-axis direction of the immersion objective; a liquid injecting unit injecting liquid between the bottom face and the distal end; and a controller controlling the liquid injecting unit, in a state where the bottom face and the distal end are disposed with a gap therebetween, to inject the liquid onto the distal end to form a droplet, and controlling the alignment unit to bring the bottom member and the immersion objective closer to each other to bring the droplet into contact with the bottom face, thereby forming a liquid column, and moving the stage and the immersion objective relative to each other while maintaining the liquid column.
    Type: Grant
    Filed: November 20, 2018
    Date of Patent: December 8, 2020
    Assignee: OLYMPUS CORPORATION
    Inventor: Yoshihiro Shimada
  • Patent number: 10754135
    Abstract: A light sheet microscope includes an objective, an illumination optical system, a first adjustor, a second adjustor and a controller. The illumination optical system irradiates sample with a light sheet from a direction that is different from an optical axis direction of the objective. The first adjustor adjusts a relative position between a light sheet plane on which the light sheet is formed and the objective in an optical axis direction of the objective. The second adjustor adjusts a relative position between the light sheet plane and the sample in an optical axis direction of the objective. The controller controls the first adjustor on the basis of light that is from the light sheet plane and that is detected via the objective when a relative position between the light sheet plane and the sample is changed by the second adjustor.
    Type: Grant
    Filed: June 21, 2017
    Date of Patent: August 25, 2020
    Assignee: OLYMPUS CORPORATION
    Inventors: Yoshihiro Shimada, Yujin Arai
  • Patent number: 10746978
    Abstract: An immersion liquid holder 2 is an immersion liquid holder that is used for an observation device 10 for observing sample S by using an immersion method, and includes a supporting member 2b configured to support an absorption member 3 that has absorbed an immersion liquid and a connection unit 2c configured to relatively fix the absorption member 3 that has absorbed the immersion liquid with respect to an optical system, which is an optical system included in the observation device 10, and to fix the absorption member 3 on an optical path of the optical system 1, by fixing the supporting member 2b to the observation device 10.
    Type: Grant
    Filed: January 16, 2018
    Date of Patent: August 18, 2020
    Assignee: OLYMPUS CORPORATION
    Inventors: Brendan Brinkman, Yoshihiro Shimada, Daisuke Nishiwaki
  • Patent number: 10684457
    Abstract: A microscope apparatus 10 includes a detection optical system 12 that captures light from a sample S and an illumination optical system 11 that radiates an illumination light onto the sample S. The illumination optical system 11 includes a cylindrical lens 5 that has a power in a first-axis direction and does not have a power in a second-axis direction that is perpendicular to the first-axis direction, a cylindrical lens 6 that has a power in the second-axis direction and does not have a power in the first-axis direction, and a scanner 4 that scans the illumination light in a width direction. The illumination optical system 11 is configured such that the first-axis direction is the width direction described above, and the cylindrical lenses 5 and 6 are arranged posterior to the scanner 4.
    Type: Grant
    Filed: January 15, 2018
    Date of Patent: June 16, 2020
    Assignee: OLYMPUS CORPORATION
    Inventors: Masahito Dohi, Yoshihiro Shimada
  • Patent number: 10509215
    Abstract: The present invention provides a light-field microscope including: an illumination optical system that radiates excitation light onto a sample; and a detection optical system including an objective lens that collects fluorescence generated in the sample as a result of the sample being irradiated with the excitation light by the illumination optical system, an image-acquisition element that acquires an image of the fluorescence collected by the objective lens, and a microlens array disposed between the image-acquisition element and the objective lens. The illumination optical system radiates a beam of the excitation light having a predetermined width in the optical-axis direction of the objective lens so as to include the focal plane of the objective lens onto the sample in a direction substantially perpendicular to the optical axis.
    Type: Grant
    Filed: February 17, 2017
    Date of Patent: December 17, 2019
    Assignee: OLYMPUS CORPORATION
    Inventors: Yoshihiro Shimada, Yujin Arai
  • Publication number: 20190310454
    Abstract: A microscope includes a movable stage supporting wells arranged in an array, a first imaging unit having a low-magnification objective lens, a second imaging unit having a high-magnification objective lens, a computer determining a representative position of a spheroid based on imaging data of the spheroid acquired by the first imaging unit, and a controller causing respective imaging units to sequentially acquire imaging data of the spheroid in each of the wells. The controller causes the first imaging unit to acquire imaging data for the spheroid in one of the wells, and then causes the stage to adjust the representative position to the optical axis of the high-magnification objective lens, and further causes the second imaging unit to acquire imaging data while causing the first imaging unit to acquire imaging data of the spheroid in another of the wells in synchronization with acquisition by the second imaging unit.
    Type: Application
    Filed: March 29, 2019
    Publication date: October 10, 2019
    Applicant: OLYMPUS CORPORATION
    Inventor: Yoshihiro SHIMADA
  • Publication number: 20190227294
    Abstract: An inverted microscope includes a stage supporting a bottom member; an immersion objective disposed facing vertically upward such that a distal end thereof faces a bottom face of the bottom member; an alignment unit moving the stage or the immersion objective in an optical-axis direction of the immersion objective; a liquid injecting unit injecting liquid between the bottom face and the distal end; and a controller controlling the liquid injecting unit, in a state where the bottom face and the distal end are disposed with a gap therebetween, to inject the liquid onto the distal end to form a droplet, and controlling the alignment unit to bring the bottom member and the immersion objective closer to each other to bring the droplet into contact with the bottom face, thereby forming a liquid column, and moving the stage and the immersion objective relative to each other while maintaining the liquid column.
    Type: Application
    Filed: November 20, 2018
    Publication date: July 25, 2019
    Applicant: OLYMPUS CORPORATION
    Inventor: Yoshihiro SHIMADA
  • Patent number: 10310248
    Abstract: Provided is a microscope including: a chamber storing a solution in which a cuvette accommodating a solution together with a sample is immersed and that has an index of refraction identical to that of the solution; an immersion objective lens being placed outside the chamber and collecting light from the sample; a camera acquiring an image of the light collected by the lens; a targeting section moving the lens in a direction along a detection light axis thereof; and a movable stage supporting the cuvette in the chamber so as to be movable in at least a direction along the detection light axis. Each of the cuvette and the chamber has a transparent section that can transmit light coming from the sample. The lens is placed so as to face the transparent section of the cuvette with the transparent section of the chamber interposed therebetween.
    Type: Grant
    Filed: August 16, 2017
    Date of Patent: June 4, 2019
    Assignee: OLYMPUS CORPORATION
    Inventors: Brendan Brinkman, Yoshihiro Shimada
  • Publication number: 20190146203
    Abstract: Provided is a sample observation method including: bringing a gel-like transparent sample that encloses an observation target into contact with a transparent flat surface section of a substrate; and collecting light from the observation target by means of an objective lens via the substrate, in a state in which a pressing force is made to act in a direction in which the sample and the flat surface section relatively approach each other, until the contact area between the sample and the flat surface section comes to have a size allowing an effective light flux for the objective lens of a microscope to pass therethrough.
    Type: Application
    Filed: November 9, 2018
    Publication date: May 16, 2019
    Applicant: OLYMPUS CORPORATION
    Inventors: Yoshihiro SHIMADA, Kazuhito GODA
  • Patent number: 10288861
    Abstract: A light sheet microscope includes an objective, an illumination optical system, a correction device, an image pickup device, a first adjustor, a second adjustor and a controller. The controller performs a first focus process and a spherical aberration correction process. The first focus process is a process in which the first adjustor is controlled on the basis of light that is from the light sheet plane and that is detected via the objective so that the light sheet plane and a focal plane become closer when a relative position between the light sheet plane and a sample has been changed. The spherical aberration correction process is a process in which the correction device is controlled so that an evaluation value of the image obtained by the image pickup device becomes greater when the first adjustor has changed the relative position between the light sheet plane and the objective.
    Type: Grant
    Filed: July 20, 2017
    Date of Patent: May 14, 2019
    Assignee: OLYMPUS CORPORATION
    Inventor: Yoshihiro Shimada
  • Patent number: 10228553
    Abstract: A microscope device 100 includes: a sample container holder 7 that is capable of housing a plurality of sample containers 9 and that is supported so as to be attachable, each of the sample containers 9 housing a sample S and a first immersion medium and including a transparent section that transmits light; an illumination optical system 1 that emits illumination light to the sample S via the transparent section; a detection optical system 2 that detects light from the sample S via the transparent section; and a movement mechanism 16 that relatively moves the sample container holder 7, the detection optical system 2, and the illumination optical system 1 in a gravity direction. The sample container holder 7 houses the plurality of sample containers S in the gravity direction.
    Type: Grant
    Filed: November 22, 2017
    Date of Patent: March 12, 2019
    Assignee: OLYMPUS CORPORATION
    Inventor: Yoshihiro Shimada
  • Publication number: 20190049709
    Abstract: A light sheet microscope includes a light source configured to output light, a plurality of optical fibers configured to guide the light that is from the light source, a plurality of illumination optical systems configured to respectively emit beams of the light guided from the plurality of optical fibers toward a sample as light sheets in a plurality of different directions on a same plane, and a light switching device that is provided between the light source and the plurality of optical fibers and that is configured to switch an optical fiber that the light output from the light source enters, from among the plurality of optical fibers.
    Type: Application
    Filed: July 31, 2018
    Publication date: February 14, 2019
    Applicant: OLYMPUS CORPORATION
    Inventors: Yoshihiro SHIMADA, Go RYU
  • Patent number: 10168520
    Abstract: Provided is a microscope system including a motor-driven stage on which is mounted a culture vessel containing one or more cell clusters, each including cells having a target molecule labeled with a fluorescent or luminescent chemical; a low-magnification-image acquiring unit that acquires low-magnification images of the cell clusters in the culture vessel mounted on the stage; a detecting unit that detects the position of each cell cluster in the culture vessel by analyzing the acquired low-magnification images; and a high-magnification-image acquiring unit that, after the detected position is aligned with the optical axis of an objective lens, acquires slice images of fluorescence or luminescence emitted from the cells forming the cell cluster at a higher magnification than the low-magnification-image acquiring unit at intervals along the optical axis while the stage and/or the lens is moved to change stepwise the distance between the lens and the cell cluster.
    Type: Grant
    Filed: May 23, 2016
    Date of Patent: January 1, 2019
    Assignee: OLYMPUS CORPORATION
    Inventors: Hisao Kitagawa, Yoshihiro Shimada, Kosuke Takagi
  • Publication number: 20180361377
    Abstract: Provided is a sample manufacturing method that includes: a step of forming a hanging drop consisting of a liquid drop of a medium solution in a hanging state while causing at least one cell aggregate to be encapsulated in the liquid drop of the medium solution, the medium solution becoming substantially transparent upon gelling or solidifying; and a step of causing the hanging drop to gel or solidify by causing a promoting factor that promotes gelling or solidification of the medium solution to act on the hanging drop.
    Type: Application
    Filed: May 21, 2018
    Publication date: December 20, 2018
    Applicant: OLYMPUS CORPORATION
    Inventors: Brendan BRINKMAN, Yoshihiro SHIMADA
  • Publication number: 20180314047
    Abstract: A microscope includes: a chamber that holds a chamber solution in which a cuvette, which contains a sample together with a cuvette solution, is immersed; an immersion objective lens that is disposed outside the chamber and that collects light coming from the sample; a detection optical system that detects the light collected by the immersion objective lens; and a motorized XYZ stage that supports the cuvette inside the chamber to be movable in a direction parallel to an optical axis of the immersion objective lens. The cuvette and the chamber each have a transparent portion that allows transmission of the light from the sample, and the immersion objective lens is disposed facing the transparent portion of the cuvette, with the transparent portion of the chamber interposed therebetween, and is disposed so that an immersion solution is interposed between said immersion objective lens and the transparent portion of the chamber.
    Type: Application
    Filed: April 5, 2018
    Publication date: November 1, 2018
    Applicant: OLYMPUS CORPORATION
    Inventor: Yoshihiro SHIMADA
  • Publication number: 20180284411
    Abstract: A microscope apparatus 10 includes a detection optical system 12 that captures light from a sample S and an illumination optical system 11 that radiates an illumination light onto the sample S. The illumination optical system 11 includes a cylindrical lens 5 that has a power in a first-axis direction and does not have a power in a second-axis direction that is perpendicular to the first-axis direction, a cylindrical lens 6 that has a power in the second-axis direction and does not have a power in the first-axis direction, and a scanner 4 that scans the illumination light in a width direction. The illumination optical system 11 is configured such that the first-axis direction is the width direction described above, and the cylindrical lenses 5 and 6 are arranged posterior to the scanner 4.
    Type: Application
    Filed: January 15, 2018
    Publication date: October 4, 2018
    Applicant: OLYMPUS CORPORATION
    Inventors: Masahito DOHI, Yoshihiro SHIMADA
  • Publication number: 20180259761
    Abstract: An immersion liquid holder 2 is an immersion liquid holder that is used for an observation device 10 for observing sample S by using an immersion method, and includes a supporting member 2b configured to support an absorption member 3 that has absorbed an immersion liquid and a connection unit 2c configured to relatively fix the absorption member 3 that has absorbed the immersion liquid with respect to an optical system, which is an optical system included in the observation device 10, and to fix the absorption member 3 on an optical path of the optical system 1, by fixing the supporting member 2b to the observation device 10.
    Type: Application
    Filed: January 16, 2018
    Publication date: September 13, 2018
    Applicant: OLYMPUS CORPORATION
    Inventors: Brendan BRINKMAN, Yoshihiro SHIMADA, Daisuke NISHIWAKI
  • Publication number: 20180224648
    Abstract: A microscope device 100 includes: a sample container holder 7 that is capable of housing a plurality of sample containers 9 and that is supported so as to be attachable, each of the sample containers 9 housing a sample S and a first immersion medium and including a transparent section that transmits light; an illumination optical system 1 that emits illumination light to the sample S via the transparent section; a detection optical system 2 that detects light from the sample S via the transparent section; and a movement mechanism 16 that relatively moves the sample container holder 7, the detection optical system 2, and the illumination optical system 1 in a gravity direction. The sample container holder 7 houses the plurality of sample containers S in the gravity direction.
    Type: Application
    Filed: November 22, 2017
    Publication date: August 9, 2018
    Applicant: OLYMPUS CORPORATION
    Inventor: Yoshihiro Shimada