Abstract: An article reversing/discharging box 1 includes a box main body 2 that accommodates scrap, hook members 3, 4 having insertion holes 3a, 4a through which forks of a forklift are inserted above the box main body 2, and a reversing mechanism 5 capable of reversing the box main body 2. The reversing mechanism 5 includes a bottom plate 6 integrally extending from a lower end of the movable hook member 4 along the bottom surface 2d, and a hinge 7 provided at a distal end portion of the bottom plate 6. When the forks are inserted into the insertion holes 4a of the movable hook member 4 and the forks are removed from the insertion holes 3a of the fixed hook members 3, the box main body 2 is turnable about the distal end portion of the bottom plate 6 by the hinge 7.

Abstract: An article reversing/discharging box 1 includes a box main body 2 that accommodates scrap, hook members 3, 4 having insertion holes 3a, 4a through which forks of a forklift are inserted above the box main body 2, and a reversing mechanism 5 capable of reversing the box main body 2. The reversing mechanism 5 includes a bottom plate 6 integrally extending from a lower end of the movable hook member 4 along the bottom surface 2d, and a hinge 7 provided at a distal end portion of the bottom plate 6. When the forks are inserted into the insertion holes 4a of the movable hook member 4 and the forks are removed from the insertion holes 3a of the fixed hook members 3, the box main body 2 is turnable about the distal end portion of the bottom plate 6 by the hinge 7.

Abstract: The press-fitting method for press-fitting a plurality of clinching fasteners into a workpiece. The method includes: a drilling step S1 of drilling press-fit holes at press-fitting positions of the workpiece to press-fit first clinching fasteners that are press-fitted from a punch side of a turret punch press by NC control using the turret punch press; a workpiece mounting step S2 of mounting the workpiece so that the workpiece is superposed on a fastening jig that guides the first clinching fasteners; a fastener insertion step S3 of inserting a predetermined number of the first clinching fasteners taken out from a screw feeder into the press-fit holes of the workpiece; a fastener press-fitting step S4 of press-fitting the first clinching fasteners into the press-fit holes of the workpiece by NC control using the turret punch press; and a workpiece removal step S5 of removing the workpiece from the fastening jig.

Abstract: The press-fitting method for press-fitting a plurality of clinching fasteners into a workpiece. The method includes: a drilling step S1 of drilling press-fit holes at press-fitting positions of the workpiece to press-fit first clinching fasteners that are press-fitted from a punch side of a turret punch press by NC control using the turret punch press; a workpiece mounting step S2 of mounting the workpiece so that the workpiece is superposed on a fastening jig that guides the first clinching fasteners; a fastener insertion step S3 of inserting a predetermined number of the first clinching fasteners taken out from a screw feeder into the press-fit holes of the workpiece; a fastener press-fitting step S4 of press-fitting the first clinching fasteners into the press-fit holes of the workpiece by NC control using the turret punch press; and a workpiece removal step S5 of removing the workpiece from the fastening jig.

Abstract: An entertainment system has a destruction displaying unit including a damage applying object processing unit for rendering an object (damage applying object) such as a robot carrying a principal character, a monster as a target, or bullets or shells shot from a weapon, which applies damage to a background object, a destruction determining unit for determining whether the background object is to be destroyed or not based on the positional relationship between the damage applying object and the background object, a background object processing unit for rendering the background object as it is being destroyed, and an image displaying unit for outputting image data rendered and stored in a frame buffer to a display monitor to display a corresponding image on the display screen of the display monitor.

Abstract: A support matrix particularly suitable for the immobilization of biologically active materials acting on lipophilic substrates results from the reaction of a polyamine-impregnated porous particle with a monofunctional paraffinic aldehyde. The resulting support matrix has a hydrophobic coating providing a microenvironment for the biologically active material, such as an enzyme, and hydrophilic portions for emulsification or binding to polar regions at or near the active site.

Abstract: A regenerable support matrix useful for immobilization of biologically active materials is prepared by coating a core support with a cellulose ester, removing ester groups by hydrolysis to produce hydroxyl groups and converting the hydroxyl groups to dialkylaminoalkyl ether groups. The support matrix can immobilize biologically active proteinaceous materials with a net negative charge by adsorption. The support matrix is readily regenerated when an immobilized biologically active material becomes inactive by washing the support with a base or salt solution and adsorbing additional biologically active material to the support. Multiple cycles of immobilization and regeneration are possible without significant deleterious affects.

Abstract: The aspartase from a mutant Bacillus subtilis, NRRL B-15536, is produced in relatively high cell yield within a comparatively short time. The enzyme converts fumaric acid to L-aspartic acid stoichiometrically with outstanding selectivity and productivity. The enzyme is stabilized by divalent magnesium ions, 2-mercaptoethanol, and ammonium furmarate, and can be conveniently purified with high recovery.

Abstract: A mutant Bacillus subtilis, arising from spontaneous adaptation from a parent without detectable aspartase activity, produces recoverable amounts of aspartase. The micro-organism attains maximum growth and enzyme production within about 8 hours and shows excellent glucose tolerance. Aspartase is at least in part produced constitutively, but L-aspartic acid stimulates further aspartase production. The aspartase so produced is readily released from the cell upon cell wall rupture and shows adequate extra-cellular stability for further concentration and purification.

Abstract: A strain of Paracoccus denitrificans, NRRL B-15710, is an overproducer of a fumarase which is readily purified and relatively thermostable. The fumarase so produced converts fumaric acid to L-malic acid without formation of any significant amounts of a coproduct.

Abstract: A process for purifying an enzyme such as glucose isomerase which comprises precipitating nucleic acids from a cell-free, heat-treated enzyme solution in a suitable buffer and chromatographing the supernatant on a cellulosic medium. Subsequent chromatography on a hydrophilic, molecular-sieve medium affords enzyme of about 90% purity.

Abstract: A process for purifying glucose isomerase comprises the steps of acid treatment and salt fractionation. An enzyme solution is treated with an acid, such as acetic acid, to a pH from about 3.5 to about 5.0. The proteinaceous solids are collected and extracted with a buffer, such as imidazole, whose solution has a pH of about 6 to about 8. The solution is then collected and a salt, such as ammonium sulfate, is dissolved therein from about 40% to about 50% of its saturation point. The proteinaceous solids which form are removed and additional ammonium sulfate is dissolved to attain from about 41% to about 60% of its saturation point, followed by collection of the solids containing purified enzyme. A composition which preserves enzyme activity upon storage of glucose isomerase and which imparts resistance to thermal deactivation of said enzyme comprises an aqueous solution of glycerol, a buffer whose solution is at a pH of about 6 to about 8, divalent cobalt ions and magnesium ions.