Abstract: The purpose of the present invention is to obtain a method for forming an excellent multi-layer coating film of high chroma and brightness and a rich color. With the method, color mottling does not occur easily in the coating film and the design obtained is homogeneous even without strict control of variations in film thickness during coating. The method for forming the excellent multi-layer coating film comprises: forming a metallic base coating film on the surface of the object to be coated by applying a metallic base coating containing a shiny material; then forming a color base coating film by applying a color base coating; subsequently forming a clear coating film by applying a clear coating on the color base coating film; and heat-curing the metallic base coating film, the color base coating film and the clear coating film obtained.

Abstract: The purpose of the present invention is to obtain a method for forming an excellent multi-layer coating film of high chroma and brightness and a rich color. With the method, color mottling does not occur easily in the coating film and the design obtained is homogeneous even without strict control of variations in film thickness during coating. The method for forming the excellent multi-layer coating film comprises: forming a metallic base coating film on the surface of the object to be coated by applying a metallic base coating containing a shiny material; then forming a color base coating film by applying a color base coating; subsequently forming a clear coating film by applying a clear coating on the color base coating film; and heat-curing the metallic base coating film, the color base coating film and the clear coating film obtained.

Abstract: An objective of the present invention is to provide aqueous preparations comprising eMIP, a derivative of macrophage inflammatory protein 1? (MIP-1?) which has an immunopotentiation activity, and stabilizer(s). The present inventors conducted dedicated studies to achieve the above-described objective. As a result, the present inventors discovered that eMIP degradation is suppressed by addition of at least one or more additives selected from sodium chloride, L-histidine, L-arginine, L-arginine hydrochloride, L-lysine hydrochloride, citric acid, and sodium edetate (EDTA). Furthermore, the present inventors demonstrated that phosphate buffer of pH 5 to pH 7 is a preferable pH adjuster for the aqueous eMIP preparations of the present invention.

Abstract: The present invention provides, as a gene encoding an antigen recognized by G-CSF-inducing antibodies, a gene encoding: (a) a protein having the amino acid sequence listed as SEQ ID NO:2 of the Sequence Listing; (b) a protein having the amino acid sequence listed as SEQ ID NO:2 of the Sequence Listing with one or more amino acid deletions, substitutions, additions or insertions and also binding to an antibody or its fragment that is active to induce granulocyte colony-stimulating factor; or (c) a protein having at least 50% homology with the amino acid sequence listed as SEQ ID NO:2 and also binding to an antibody or its fragment that is active to induce granulocyte colony-stimulating factor.

Abstract: The present invention provides, as a gene encoding an antigen recognized by a G-CSF inducing antibody, a gene encoding any one of the following proteins: (a) a protein having an amino acid sequence shown in SEQ ID NO; 2; (b) a protein having an amino acid sequence comprising a deletion, substitution, addition or insertion of one or several amino acids with respect to the amino acid sequence shown in SEQ ID NO: 2, and having a binding ability for an antibody which induces and secretes a granulocyte colony-stimulating factor or a fragment thereof: or (c) a protein having at least 50% or more homology with the amino acid sequence shown in SEQ ID NO: 2, and having a binding ability for an antibody capable of inducing and secreting a granulocyte colony-stimulating factor or a fragment thereof.

Abstract: The present invention provides, as a gene encoding an antigen recognized by G-CSF-inducing antibodies, a gene encoding: (a) a protein having the amino acid sequence listed as SEQ ID NO:2 of the Sequence Listing; (b) a protein having the amino acid sequence listed as SEQ ID NO:2 of the Sequence Listing with one or more amino acid deletions, substitutions, additions or insertions and also binding to an antibody or its fragment that is active to induce granulocyte colony-stimulating factor; or (c) a protein having at least 50% homology with the amino acid sequence listed as SEQ ID NO:2 and also binding to an antibody or its fragment that is active to induce granulocyte colony-stimulating factor.

Abstract: The present invention provides, as a gene encoding an antigen recognized by a G-CSF inducing antibody, a gene encoding any one of the following proteins: (a) a protein having an amino acid sequence shown in SEQ ID NO; 2; (b) a protein having an amino acid sequence comprising a deletion, substitution, addition or insertion of one or several amino acids with respect to the amino acid sequence shown in SEQ ID NO: 2, and having a binding ability for an antibody which induces and secretes a granulocyte colony-stimulating factor or a fragment thereof: or (c) a protein having at least 50% or more homology with the amino acid sequence shown in SEQ ID NO: 2, and having a binding ability for an antibody capable of inducing and secreting a granulocyte colony-stimulating factor or a fragment thereof.