Patents by Inventor Yoshimi Kikuchi

Yoshimi Kikuchi has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 9376701
    Abstract: A novel technique for improving secretory production of a heterologous protein by coryneform bacteria is described, and thereby a method for secretory production of a heterologous protein is provided. A coryneform bacterium is cultured so that it secretes a heterologous protein, the bacterium having a genetic construct which includes a promoter sequence that functions in the coryneform bacterium, a nucleic acid sequence coding for a signal peptide that functions in the coryneform bacterium, which is ligated downstream from the promoter sequence, and a nucleic acid sequence coding for a fusion protein having an amino acid sequence that includes Gln-Glu-Thr and the heterologous protein, which is ligated downstream from the nucleic acid sequence coding for the signal peptide.
    Type: Grant
    Filed: April 11, 2014
    Date of Patent: June 28, 2016
    Assignee: AJINOMOTO CO., INC.
    Inventors: Noriko Tsurui, Hiroshi Itaya, Yoshimi Kikuchi
  • Patent number: 9187554
    Abstract: A method for secretory production of a heterologous protein is provided by developing a novel technique for improving ability of a coryneform bacterium to produce a heterologous protein by secretory production. By utilizing a coryneform bacterium having an ability to produce a heterologous protein by secretory production which has been modified so that the activity of a penicillin-binding protein is reduced and in which the activity of a cell surface layer protein has been reduced as an expression host, a heterologous protein is produced by secretory production.
    Type: Grant
    Filed: April 29, 2014
    Date of Patent: November 17, 2015
    Assignee: AJINOMOTO CO., INC.
    Inventors: Yoshihiko Matsuda, Hiroshi Itaya, Yoshimi Kikuchi, Haruki Beppu, Jurgis Antanas Vladovich Jomantas, Ekaterina Aleksandrovna Kutukova
  • Publication number: 20140255996
    Abstract: A method for secretory production of a heterologous protein is provided by developing a novel technique for improving ability of a coryneform bacterium to produce a heterologous protein by secretory production. By utilizing a coryneform bacterium having an ability to produce a heterologous protein by secretory production which has been modified so that the activity of a penicillin-binding protein is reduced and in which the activity of a cell surface layer protein has been reduced as an expression host, a heterologous protein is produced by secretory production.
    Type: Application
    Filed: April 29, 2014
    Publication date: September 11, 2014
    Applicant: AJINOMOTO CO., INC.
    Inventors: Yoshihiko Matsuda, Hiroshi Itaya, Yoshimi Kikuchi, Haruki Beppu, Jurgis Antanas Vladovich Jomantas, Ekaterina Aleksandrovna Kutukova
  • Publication number: 20140220637
    Abstract: A novel technique for improving secretory production of a heterologous protein by coryneform bacteria is described, and thereby a method for secretory production of a heterologous protein is provided. A coryneform bacterium is cultured so that it secretes a heterologous protein, the bacterium having a genetic construct which includes a promoter sequence that functions in the coryneform bacterium, a nucleic acid sequence coding for a signal peptide that functions in the coryneform bacterium, which is ligated downstream from the promoter sequence, and a nucleic acid sequence coding for a fusion protein having an amino acid sequence that includes Gln-Glu-Thr and the heterologous protein, which is ligated downstream from the nucleic acid sequence coding for the signal peptide.
    Type: Application
    Filed: April 11, 2014
    Publication date: August 7, 2014
    Applicant: AJINOMOTO CO., INC.
    Inventors: Noriko Tsurui, Hiroshi Itaya, Yoshimi Kikuchi
  • Patent number: 8674687
    Abstract: An angle detection device of the present invention includes: a stator which includes a plurality of salient pole portions which are formed on a flat sheet made of a magnetic material and are raised by bending, each salient pole portion constituting a winding magnetic core on which a winding member for excitation and a winding member for detection are mounted; and a rotor which is made of a magnetic material and is provided rotatably relative to the stator such that gap permeance between the rotor and each salient pole portion is changed due to the rotation of the rotor about a rotational axis of the rotor. According to the angle detection device of the present invention, it is possible to provide the angle detection device which can largely reduce the number of parts and can realize the reduction of cost and the enhancement of reliability.
    Type: Grant
    Filed: June 9, 2009
    Date of Patent: March 18, 2014
    Assignee: Tamagawa Seiki Co., Ltd.
    Inventors: Yoshimi Kikuchi, Kanji Kitazawa, Hisashi Mimura
  • Patent number: 8669760
    Abstract: An angle detection system of the present invention includes: a stator 200 which includes a plurality of salient pole portions which are formed on a flat sheet made of a magnetic material and are raised by bending, each salient pole portion constituting a winding magnetic core on which a winding member for excitation and a winding member for detection are mounted; and a rotor 300 which is made of a magnetic material and is configured to be rotatable relative to the stator such that gap permeance between the rotor and each salient pole portion is changed due to the rotation thereof about a rotational axis thereof. According to the angle detection device of the present invention, it is possible to provide the angle detection device which can largely reduce the number of parts and can realize the reduction of cost and the enhancement of reliability.
    Type: Grant
    Filed: May 9, 2009
    Date of Patent: March 11, 2014
    Assignee: Tamagawa Seiki Co., Ltd.
    Inventors: Yoshimi Kikuchi, Kanji Kitazawa, Hisashi Mimura, Minow Okada
  • Patent number: 8597907
    Abstract: The present invention provides a method for efficiently producing an industrially useful protein in coryneform bacteria, and more particularly, a method for efficiently producing a protein for which secretion was difficult using conventional protein secretion pathways. In particular, the present invention provides a method for efficiently producing heterologous proteins comprising culturing coryneform bacteria containing an genetic construction containing a promoter sequence which functions in coryneform bacteria, a nucleic acid sequence encoding a Tat system-dependent signal peptide region, and a nucleic acid sequence encoding a heterologous protein, in the direction from 5?-end to 3?-end, and secretory producing the heterologous protein by coryneform bacteria.
    Type: Grant
    Filed: October 19, 2006
    Date of Patent: December 3, 2013
    Assignee: Ajinomoto Co., Inc.
    Inventors: Masayo Date, Yoshimi Kikuchi, Hiroshi Itaya, Nami Nakamura
  • Publication number: 20120311850
    Abstract: A resolver (100) used for a rotational angle detecting device is provided with a stator (200) which is formed with a plurality of annularly-linked stator teeth (210) and a rotor (300) which is rotatably provided with respect to the stator (200). A group of output windings which have output windings connected in series in order to obtain an output signal corresponding to the rotational angle of the rotor (300) are wound about the stator teeth (210). When a number is assigned to each stator tooth (210) in accordance with the order of placement of the stator tooth (210), the number of windings W(k) of the output windings wound about the k-th stator tooth (210) is defined by the following formula (1). Thus, with respect to methods of detecting a rotational angle or winding synchronizing device windings in order to output a sinusoidal signal from the group of output windings, the number of windings can be easily set compared to in the past.
    Type: Application
    Filed: March 29, 2010
    Publication date: December 13, 2012
    Inventors: Yonezou Kubota, Yoshimi Kikuchi
  • Patent number: 8187835
    Abstract: By culturing a methanol-assimilating bacterium which harbors a DNA construct which contains a promoter sequence that functions in the methanol-assimilating bacterium and a nucleotide sequence that encodes a polypeptide containing a signal sequence and an objective protein which is functionally connected to the promoter sequence, in a liquid medium containing methanol as a major carbon source, the bacterium is allowed to secrete the objective protein, and the secreted objective protein is recovered.
    Type: Grant
    Filed: November 13, 2007
    Date of Patent: May 29, 2012
    Assignee: Ajinomoto Co., Inc.
    Inventors: Hiroshi Itaya, Yoshimi Kikuchi, Masayo Date
  • Patent number: 8105802
    Abstract: The present invention provides a neutral metalloprotease from actinomycetes which selectively cleaves a pro-structure part of a microbial protransglutaminase and a gene encoding said neutral metalloprotease. An active microbial transglutaminase having the pro-structure part cleaved can be obtained by culturing a microorganism into which a gene encoding the neutral metalloprotease from actinomycetes according to the present invention has been introduced, where by producing the neutral metalloprotease from actinomycetes, and reacting it on a microbial protransglutaminase.
    Type: Grant
    Filed: March 1, 2010
    Date of Patent: January 31, 2012
    Assignee: Ajinomoto Co., Inc.
    Inventors: Yukiko Umezawa, Keiichi Yokoyama, Yoshimi Kikuchi, Masayo Date, Norimasa Onishi
  • Patent number: 8093346
    Abstract: Provided is a method for efficiently producing a 3-amino-4-hydroxybenzoic acid-type compound by culturing a coryneform bacterium that has a gene encoding a mutated aspartokinase not subject to feedback inhibition, and that is transformed with a recombinant vector containing a DNA encoding a protein having an activity to form 3-amino-4-hydroxybenzoic acid from dihydroxyacetone phosphate and aspartate semialdehyde.
    Type: Grant
    Filed: January 3, 2011
    Date of Patent: January 10, 2012
    Assignee: Ajinomoto Co., Inc.
    Inventors: Mototaka Suzuki, Keiichi Yokoyama, Yoshimi Kikuchi
  • Publication number: 20110285386
    Abstract: An angle detection system of the present invention includes: a stator 200 which includes a plurality of salient pole portions which are formed on a flat sheet made of a magnetic material and are raised by bending, each salient pole portion constituting a winding magnetic core on which a winding member for excitation and a winding member for detection are mounted; and a rotor 300 which is made of a magnetic material and is configured to be rotatable relative to the stator such that gap permeance between the rotor and each salient pole portion is changed due to the rotation thereof about a rotational axis thereof. According to the angle detection device of the present invention, it is possible to provide the angle detection device which can largely reduce the number of parts and can realize the reduction of cost and the enhancement of reliability.
    Type: Application
    Filed: May 9, 2009
    Publication date: November 24, 2011
    Applicant: TAMAGAWA SEIKI CO., LTD.
    Inventors: Yoshimi Kikuchi, Masahito Okada, Kanji Kitazawa, Hisashi Mimura
  • Patent number: 8062869
    Abstract: An Escherichia bacterium having dihydrodipicolinate synthase and aspartokinase, both of which are desensitized to feedback inhibition by L-lysine. The intracellular activity of dihydrodipicolinate reductase in this bacterium can also be enhanced. Furthermore, a diaminopimelate dehydrogenase gene can be introduced into this bacterium, or intracellular activities of tetrahydrodipicolinate succinylase and succinyl diaminopimelate deacylase can be enhanced. Finally, the intracellular activities of aspartate-semialdehyde dehydrogenase or phosphoenolpyruvate carboxylase can be enhanced in this bacterium. The bacterium can be cultured in a suitable medium to produce and accumulate L-lysine in culture, and the L-lysine is collected from the culture.
    Type: Grant
    Filed: March 11, 2010
    Date of Patent: November 22, 2011
    Assignee: Ajinomoto Co., Inc.
    Inventors: Kazuo Nakanishi, Yoshimi Kikuchi, Junichiro Kojima, Tomoko Suzuki, Yasushi Nishimura, Hiroyuki Kojima
  • Patent number: 7972829
    Abstract: The present invention relates to a process for secretory production of a foreign protein, in particular, transglutaminase by a coryneform bacterium. According to the present invention, a process is provided for the secretory production of a foreign protein, in particular, transglutaminase, by making a coryneform bacterium to produce an industrially useful foreign protein, in particular, transglutaminase and efficiently release the product extracellularly (i.e., secretory production).
    Type: Grant
    Filed: February 26, 2010
    Date of Patent: July 5, 2011
    Assignee: Ajinomoto Co., Inc.
    Inventors: Yoshimi Kikuchi, Masayo Date, Yukiko Umezawa, Keiichi Yokoyama, Hiroshi Matsui
  • Publication number: 20110133965
    Abstract: An angle detection device of the present invention includes: a stator which includes a plurality of salient pole portions which are formed on a flat sheet made of a magnetic material and are raised by bending, each salient pole portion constituting a winding magnetic core on which a winding member for excitation and a winding member for detection are mounted; and a rotor which is made of a magnetic material and is provided rotatably relative to the stator such that gap permeance between the rotor and each salient pole portion is changed due to the rotation of the rotor about a rotational axis of the rotor. According to the angle detection device of the present invention, it is possible to provide the angle detection device which can largely reduce the number of parts and can realize the reduction of cost and the enhancement of reliability.
    Type: Application
    Filed: June 9, 2009
    Publication date: June 9, 2011
    Applicant: TAMAGAWA SEIKI CO., LTD.
    Inventors: Yoshimi Kikuchi, Kanji Kitazawa, Hisashi Mimura
  • Publication number: 20110137007
    Abstract: Provided is a method for efficiently producing a 3-amino-4-hydroxybenzoic acid-type compound by culturing a coryneform bacterium that has a gene encoding a mutated aspartokinase not subject to feedback inhibition, and that is transformed with a recombinant vector containing a DNA encoding a protein having an activity to form 3-amino-4-hydroxybenzoic acid from dihydroxyacetone phosphate and aspartate semialdehyde.
    Type: Application
    Filed: January 3, 2011
    Publication date: June 9, 2011
    Inventors: Mototaka Suzuki, Keiichi Yokoyama, Yoshimi Kikuchi
  • Patent number: 7852069
    Abstract: An angle detector including an annular stator board (1); a plurality of stators (5) provided on the annular stator board (1) independently of one another and forming slots (10) at constant or various intervals; a magnetic core (11) formed on surfaces of the stators (5); stator windings (4) formed around the magnetic cores (11) and composed of excitation windings (4a) and output windings (4b); a magnetic flux return path plateau (12) provided on each of the surfaces of the stators (5) and provided inward or outward of the stator windings (4); an annular rotor board (6) provided on the magnetic flux return path plateaus (12); and a gap forming board (13) provided on an inner surface (6a) of the annular rotor board (6) and having a board inner surface (13a) whose protrusion state changes according to a waveform. The annular rotor board rotates to cause changes in a gap (14) between each of the magnetic cores (11) and the board inner surface (13a) of the gap forming board (13).
    Type: Grant
    Filed: April 24, 2007
    Date of Patent: December 14, 2010
    Assignee: Tamagawa Seiki Kabushiki Kaisha
    Inventors: Yoshimi Kikuchi, Kanji Kitazawa, Hisashi Mimura, Masahito Okada, Yonezo Kubota
  • Publication number: 20100297729
    Abstract: The present invention relates to a process for secretory production of a foreign protein, in particular, transglutaminase by a coryneform bacterium. According to the present invention, a process is provided for the secretory production of a foreign protein, in particular, transglutaminase, by making a coryneform bacterium to produce an industrially useful foreign protein, in particular, transglutaminase and efficiently release the product extracellularly (i.e., secretory production).
    Type: Application
    Filed: February 26, 2010
    Publication date: November 25, 2010
    Applicant: AJINOMOTO CO., INC
    Inventors: Yoshimi Kikuchi, Masayo Date, Yukiko Umezawa, Keiichi Yokoyama, Hiroshi Matsui
  • Patent number: 7816912
    Abstract: An angle detector includes a magnetic rotor and a plurality of magnetic stator portions. The magnetic stator portions are arranged separately in an annular stator frame and each magnetic stator portion includes a pair of magnetic stator strips. The periphery of the magnetic rotor is disposed between the magnetic stator strips of each magnetic stator portion. By such a configuration, accurate angle detection can be carried out irrespective of the decentering of a rotary shaft.
    Type: Grant
    Filed: April 16, 2007
    Date of Patent: October 19, 2010
    Assignee: Tamagawa Seiki Kabushiki Kaisha
    Inventors: Yoshimi Kikuchi, Kanji Kitazawa, Hisashi Mimura
  • Publication number: 20100173368
    Abstract: An Escherichia bacterium having dihydrodipicolinate synthase and aspartokinase, both of which are desensitized to feedback inhibition by L-lysine. The intracellular activity of dihydrodipicolinate reductase in this bacterium can also be enhanced. Furthermore, a diaminopimelate dehydrogenase gene can be introduced into this bacterium, or intracellular activities of tetrahydrodipicolinate succinylase and succinyl diaminopimelate deacylase can be enhanced. Finally, the intracellular activities of aspartate-semialdehyde dehydrogenase or phosphoenolpyruvate carboxylase can be enhanced in this bacterium. The bacterium can be cultured in a suitable medium to produce and accumulate L-lysine in culture, and the L-lysine is collected from the culture.
    Type: Application
    Filed: March 11, 2010
    Publication date: July 8, 2010
    Inventors: Kazuo Nakanishi, Yoshimi Kikuchi, Junichiro Kojima, Tomoko Suzuki, Yasushi Nishimura, Hiroyuki Kojima