Patents by Inventor Yoshimitsu Takakura
Yoshimitsu Takakura has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
-
Publication number: 20240032498Abstract: Provided are a tobacco plant in which a high solavetivone content can be achieved without inoculation with a virus, and a method for producing the tobacco plant. In a tobacco plant in accordance with an aspect of the present invention, a function of an endogenous CA1 gene is suppressed.Type: ApplicationFiled: August 25, 2023Publication date: February 1, 2024Applicant: JAPAN TOBACCO INC.Inventors: Takanori TAKEUCHI, Hisashi UDAGAWA, Yoshimitsu TAKAKURA, Hiroshi MAGOME, Ryo NISHIGUCHI
-
Patent number: 11659807Abstract: In order to provide tobacco resistant to a virus, tobacco in accordance with the present invention is arranged such that: (i) a translation initiation factor eIF(iso)4E protein which is non-functional with respect to a virus is produced or (ii) expression of a translation initiation factor eIF(iso)4E gene is suppressed; and (a) a translation initiation factor eIF4E2 protein, which is non-functional with respect to a virus, is produced or (b) expression of a translation initiation factor eIF4E2 gene is suppressed.Type: GrantFiled: February 25, 2019Date of Patent: May 30, 2023Assignee: JAPAN TOBACCO INC.Inventors: Yoshimitsu Takakura, Akira Shinjo, Hisashi Udagawa, Kazuharu Koga
-
Publication number: 20230120622Abstract: An embodiment of the present invention provides a tobacco plant having a low alkaloid content. In the tobacco plant, a function of a gene encoding aspartate oxidase AO2 is suppressed.Type: ApplicationFiled: October 13, 2022Publication date: April 20, 2023Applicant: JAPAN TOBACCO INC.Inventors: Takanori TAKEUCHI, Masao ARAI, Hisashi UDAGAWA, Hiroshi MAGOME, Ryo NISHIGUCHI, Yoshimitsu TAKAKURA
-
Publication number: 20230071752Abstract: The object of an embodiment of the present invention is to improve the quality of a tobacco product. The present invention relates to a tobacco plant in which a mutation is introduced in a genome, the mutation causing suppression of a function of a specific endogenous gene.Type: ApplicationFiled: October 19, 2022Publication date: March 9, 2023Applicant: JAPAN TOBACCO INC.Inventors: Hiroshi MAGOME, Masao ARAI, Kiyoshi OYAMA, Yoshimitsu TAKAKURA, Ryo NISHIGUCHI
-
Patent number: 10400251Abstract: A virus-resistant tobacco in accordance with the present invention is such that it includes a mutation in a translation initiation factor eIF(iso)4E gene, the mutation causing production of an eIF(iso)4E protein which is non-functional with respect to a virus or suppressing expression of the eIF(iso)4E gene, or a virus-resistant tobacco in accordance with the present invention is such that an expression level of the eIF(iso)4E gene is 20% or lower as compared to a wild type.Type: GrantFiled: December 21, 2016Date of Patent: September 3, 2019Assignee: JAPAN TOBACCO INC.Inventors: Yoshimitsu Takakura, Kazuharu Koga, Akira Shinjo, Hisashi Udagawa
-
Publication number: 20190174706Abstract: In order to provide tobacco resistant to a virus, tobacco in accordance with the present invention is arranged such that: (i) a translation initiation factor eIF(iso)4E protein which is non-functional with respect to a virus is produced or (ii) expression of a translation initiation factor eIF(iso)4E gene is suppressed; and (a) a translation initiation factor eIF4E2 protein, which is non-functional with respect to a virus, is produced or (b) expression of a translation initiation factor eIF4E2 gene is suppressed.Type: ApplicationFiled: February 25, 2019Publication date: June 13, 2019Applicant: JAPAN TOBACCO INC.Inventors: Yoshimitsu TAKAKURA, Akira SHINJO, Hisashi UDAGAWA, Kazuharu KOGA
-
Publication number: 20170107533Abstract: A virus-resistant tobacco in accordance with the present invention is such that it includes a mutation in a translation initiation factor eIF(iso)4E gene, the mutation causing production of an eIF(iso)4E protein which is non-functional with respect to a virus or suppressing expression of the eIF(iso)4E gene, or a virus-resistant tobacco in accordance with the present invention is such that an expression level of the eIF(iso)4E gene is 20% or lower as compared to a wild type.Type: ApplicationFiled: December 21, 2016Publication date: April 20, 2017Applicant: JAPAN TOBACCO INC.Inventors: Yoshimitsu TAKAKURA, Kazuharu KOGA, Akira SHINJO, Hisashi UDAGAWA
-
Patent number: 9611303Abstract: The present invention provides a modified biotin-binding protein comprising an amino acid sequence represented by SEQ ID NO: 2 or its modified sequence and having a biotin-binding activity and replacement selected from the group consisting of: 1) replacement of the 36th serine residue of SEQ ID NO: 2 with an amino acid residue that does not form a hydrogen bond; 2) replacement of the 80th tryptophan residue of SEQ ID NO: 2 with a hydrophilic amino acid residue; 3) replacement of the 116th aspartic acid residue of SEQ ID NO: 2 with an amino acid residue that does not form a hydrogen bond; 4) replacement of the 46th proline residue of SEQ ID NO: 2 with a threonine, serine, or tyrosine residue and replacement of the 78th threonine residue of SEQ ID NO: 2 with an amino acid residue that does not form a hydrogen bond; 5) replacement of the 46th proline residue of SEQ ID NO: 2 with a threonine, serine, or tyrosine residue and replacement of the 116th aspartic acid residue of SEQ ID NO: 2 with an amino acid thatType: GrantFiled: March 18, 2015Date of Patent: April 4, 2017Assignee: JAPAN TOBACCO INC.Inventors: Yoshimitsu Takakura, Masako Tsunashima, Kozue Sofuku
-
Patent number: 9573982Abstract: The present invention provides modified tamabidin 2, which is a modified biotin-binding protein comprising an amino acid sequence represented by SEQ ID NO: 2, an amino acid sequence having one or more amino acid mutations in the amino acid sequence of SEQ ID NO: 2, or an amino acid sequence having an identity of not less than 80% to the amino acid sequence of SEQ ID NO: 2 and having biotin-binding activity, wherein an asparagine residue at position 115 of SEQ ID NO: 2 is replaced with cysteine. The modified biotin-binding protein has remarkable heat resistance.Type: GrantFiled: December 28, 2011Date of Patent: February 21, 2017Assignee: JAPAN TOBACCO INC.Inventors: Yoshimitsu Takakura, Naomi Oka
-
Patent number: 9556450Abstract: Isolated polynucleotides and polypeptides and recombinant DNA constructs useful for conferring drought tolerance, compositions (such as plants or seeds) comprising these recombinant DNA constructs, and methods utilizing these recombinant DNA constructs. The recombinant DNA construct comprises a polynucleotide operably linked to a promoter that is functional in a plant, wherein said polynucleotide encodes a DTP21 polypeptide.Type: GrantFiled: December 15, 2014Date of Patent: January 31, 2017Assignees: E I DU PONT DE NEMOURS AND COMPANY, JAPAN TOBACCO INC, PIONEER HI-BRED INTL INC.Inventors: Marc C Albertsen, Yukoh Hiei, Teruyuki Imayama, Masakazu Kashihara, Norio Kato, Toshihiko Komari, Toshiyuki Komori, Stanley Luck, Ichiro Oka, Hajime Sakai, Yoshimitsu Takakura, Scott V Tingey, Satoru Usami
-
Publication number: 20150291671Abstract: The present invention provides a modified biotin-binding protein comprising an amino acid sequence represented by SEQ ID NO: 2 or its modified sequence and having a biotin-binding activity and replacement selected from the group consisting of: 1) replacement of the 36th serine residue of SEQ ID NO: 2 with an amino acid residue that does not form a hydrogen bond; 2) replacement of the 80th tryptophan residue of SEQ ID NO: 2 with a hydrophilic amino acid residue; 3) replacement of the 116th aspartic acid residue of SEQ ID NO: 2 with an amino acid residue that does not form a hydrogen bond; 4) replacement of the 46th proline residue of SEQ ID NO: 2 with a threonine, serine, or tyrosine residue and replacement of the 78th threonine residue of SEQ ID NO: 2 with an amino acid residue that does not form a hydrogen bond; 5) replacement of the 46th proline residue of SEQ ID NO: 2 with a threonine, serine, or tyrosine residue and replacement of the 116th aspartic acid residue of SEQ ID NO: 2 with an amino acid thatType: ApplicationFiled: March 18, 2015Publication date: October 15, 2015Applicant: JAPAN TOBACCO INC.Inventors: Yoshimitsu TAKAKURA, Masako TSUNASHIMA, Kozue SOFUKU
-
Patent number: 9139617Abstract: The present invention relates to a method for detecting an antibody against a small protein encoded by the intermediate stage transcript of HHV-6 (SITH-1) in a biological sample. The method of the present invention includes the following steps: 1) providing the SITH-1 protein; 2) binding the SITH-1 protein provided in step 1) to a carrier; and 3) contacting the biological sample with the SITH-1 protein-bound carrier prepared in step 2) to detect the SITH-1 protein antibody.Type: GrantFiled: March 31, 2010Date of Patent: September 22, 2015Assignees: JAPAN TOBACCO INC., VIRUS IKAGAKU KENKYUSHO INC.Inventors: Yoshimitsu Takakura, Naomi Oka, Kazuhiro Kondo, Nobuyuki Kobayashi
-
Patent number: 9034590Abstract: The present invention provides a method for detecting a substance in a biological sample, a carrier for using in the method, and a kit.Type: GrantFiled: March 31, 2010Date of Patent: May 19, 2015Assignees: JAPAN TOBACCO INC., VIRUS IKAGAKU KENKYUSHO INC.Inventors: Yoshimitsu Takakura, Naomi Oka, Kazuhiro Kondo
-
Patent number: 9018360Abstract: The present invention provides a modified biotin-binding protein comprising an amino acid sequence represented by SEQ ID NO: 2 or its modified sequence and having a biotin-binding activity and replacement selected from the group consisting of: 1) replacement of the 36th serine residue of SEQ ID NO: 2 with an amino acid residue that does not form a hydrogen bond; 2) replacement of the 80th tryptophan residue of SEQ ID NO: 2 with a hydrophilic amino acid residue; 3) replacement of the 116th aspartic acid residue of SEQ ID NO: 2 with an amino acid residue that does not form a hydrogen bond; 4) replacement of the 46th proline residue of SEQ ID NO: 2 with a threonine, serine, or tyrosine residue and replacement of the 78th threonine residue of SEQ ID NO: 2 with an amino acid residue that does not form a hydrogen bond; 5) replacement of the 46th proline residue of SEQ ID NO: 2 with a threonine, serine, or tyrosine residue and replacement of the 116th aspartic acid residue of SEQ ID NO: 2 with an amino acid thatType: GrantFiled: June 24, 2009Date of Patent: April 28, 2015Assignee: Japan Tobacco Inc.Inventors: Yoshimitsu Takakura, Masako Tsunashima, Kozue Sofuku
-
Publication number: 20150101078Abstract: Isolated polynucleotides and polypeptides and recombinant DNA constructs useful for conferring drought tolerance, compositions (such as plants or seeds) comprising these recombinant DNA constructs, and methods utilizing these recombinant DNA constructs. The recombinant DNA construct comprises a polynucleotide operably linked to a promoter that is functional in a plant, wherein said polynucleotide encodes a DTP21 polypeptide.Type: ApplicationFiled: December 15, 2014Publication date: April 9, 2015Inventors: MARC C ALBERTSEN, Yukoh Hiei, Teruyuki Imayama, Masakazu Kashihara, Norio Kato, Toshihiko Komari, Toshiyuki Komori, Stanley Luck, Ichiro Oka, Hajime Sakai, Yoshimitsu Takakura, Scott V Tingey, Satoru Usami
-
Patent number: 8957190Abstract: The present invention relates to a solid support having a heat-resistant biotin-binding protein attached thereto. The present invention also relates to the use of the solid support of the present invention having a heat-resistant biotin-binding protein attached thereto. The present invention further relates to technical fields such as purification, concentration, detection and/or capture of a biotin-linked substance by means of a heat-resistant biotin-binding protein. Such a biotin-binding protein used in the solid support of the present invention is heat-resistant and is therefore useful for use in assay systems involving exposure to a temperature of 70° C. or more.Type: GrantFiled: July 11, 2013Date of Patent: February 17, 2015Assignee: Japan Tobacco Inc.Inventors: Yoshimitsu Takakura, Satoru Usami, Masako Ichikawa
-
Patent number: 8916746Abstract: Isolated polynucleotides and polypeptides and recombinant DNA constructs useful for conferring drought tolerance, compositions (such as plants or seeds) comprising these recombinant DNA constructs, and methods utilizing these recombinant DNA constructs. The recombinant DNA construct comprises a polynucleotide operably linked to a promoter that is functional in a plant, wherein said polynucleotide encodes a DTP21 polypeptide.Type: GrantFiled: October 29, 2010Date of Patent: December 23, 2014Assignees: Japan Tobacco, Inc., E. I. du Pont de Neumours and Company, Pioneer Hi-Bred International, Inc.Inventors: Masakazu Kashihara, Toshiyuki Komori, Ichiro Oka, Satoru Usami, Norio Kato, Yukoh Hiei, Yoshimitsu Takakura, Toshihiko Komari, Teruyuki Imayama, Scott V. Tingey, Hajime Sakai, Marc C. Albertsen, Stanley Luck
-
Publication number: 20140242620Abstract: The present invention provides a method of inhibiting non-specific binding in a step of detecting a substance in a biological sample. The invention provides a method of inhibiting non-specific binding in a step of detecting a substance in a biological sample, the method comprising: bringing a biotin-binding protein monomer not substantially having biotin-binding ability into contact with the biological sample, wherein in the step of detecting, a substance capable of specifically binding to the substance to be detected is immobilized to a carrier through binding between biotin and the biotin-binding protein before or after binding of the substance to be detected to the substance capable of specifically binding to the substance to be detected.Type: ApplicationFiled: August 1, 2012Publication date: August 28, 2014Applicant: JAPAN TOBACCO INC.Inventors: Yoshimitsu Takakura, Naomi Oka
-
Publication number: 20130309691Abstract: The present invention relates to a solid support having a heat-resistant biotin-binding protein attached thereto. The present invention also relates to the use of the solid support of the present invention having a heat-resistant biotin-binding protein attached thereto. The present invention further relates to technical fields such as purification, concentration, detection and/or capture of a biotin-linked substance by means of a heat-resistant biotin-binding protein. Such a biotin-binding protein used in the solid support of the present invention is heat-resistant and is therefore useful for use in assay systems involving exposure to a temperature of 70° C. or more.Type: ApplicationFiled: July 11, 2013Publication date: November 21, 2013Applicant: JAPAN TOBACCO INC.Inventors: Yoshimitsu TAKAKURA, Satoru USAMI, Masako ICHIKAWA
-
Publication number: 20130309765Abstract: The present invention provides modified tamabidin 2, which is a modified biotin-binding protein comprising an amino acid sequence represented by SEQ ID NO: 2, an amino acid sequence having one or more amino acid mutations in the amino acid sequence of SEQ ID NO: 2, or an amino acid sequence having an identity of not less than 80% to the amino acid sequence of SEQ ID NO: 2 and having biotin-binding activity, wherein an asparagine residue at position 115 of SEQ ID NO: 2 is replaced with cysteine. The modified biotin-binding protein has remarkable heat resistance.Type: ApplicationFiled: December 28, 2011Publication date: November 21, 2013Applicant: JAPAN TOBACCO INC.Inventors: Yoshimitsu Takakura, Naomi Oka