Patents by Inventor Yoshinobu Okuno
Yoshinobu Okuno has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 9605053Abstract: Provided is an anti-influenza virus antibody that exhibits neutralizing activity beyond the barrier of the two groups of influenza viruses categorized according to the conservativeness of hemagglutinin amino acids, a method of producing the same, and a test method for determining whether the subject carries the neutralizing antibody.Type: GrantFiled: February 5, 2016Date of Patent: March 28, 2017Assignees: Fujita Health University, The Research Foundation for Microbial Diseases of Osaka UniversityInventors: Yoshikazu Kurosawa, Yoshitaka Iba, Nobuko Ohshima, Yoshinobu Okuno
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Patent number: 9534042Abstract: Provided is an anti-influenza virus antibody that exhibits neutralizing activity beyond the barrier of the two groups of influenza viruses categorized according to the conservativeness of hemagglutinin amino acids, a method of producing the same, and a test method for determining whether the subject carries the neutralizing antibody.Type: GrantFiled: August 4, 2011Date of Patent: January 3, 2017Assignees: Fujita Health University, The Research Foundation for Microbial Diseases of Osaka UniversityInventors: Yoshikazu Kurosawa, Yoshitaka Iba, Nobuko Ohshima, Yoshinobu Okuno
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Patent number: 9493550Abstract: Provided is a human antibody having a neutralization activity against a human influenza virus. More specifically, provided is a human antibody which recognizes a highly conserved region in a human influenza A virus subtype H3N2 or a human influenza B virus and has a neutralization activity against the virus. The human antibody is a human anti-human influenza virus antibody, which has a neutralization activity against a human influenza A virus subtype H3N2 and binds to a hemagglutinin HA1 region of the human influenza A virus subtype H3N2, or which has a neutralization activity against a human influenza B virus, and includes, as a base sequence of a DNA encoding a variable region of the antibody, a sequence set forth in any one of SEQ ID NOS: 5 to 12.Type: GrantFiled: October 3, 2014Date of Patent: November 15, 2016Assignees: Osaka University, The Research Foundation for Microbial Diseases of Osaka UniversityInventors: Kazuyoshi Ikuta, Ritsuko Koketsu, Yoshinobu Okuno, Mikihiro Yunoki, Shoji Ideno, Masatoshi Oshita, Motoki Kuhara, Masatoshi Momota
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Publication number: 20160200799Abstract: It is an object of the present invention to provide a novel antibody having a high binding activity and a high neutralizing activity on influenza viruses. The present invention provides an antibody, which neutralizes H1 influenza virus and/or H5 influenza virus, wherein the antibody has a heavy chain variable region having CDRs consisting of a defined heavy chain first complementarity-determining region (VH CDR1), a defined heavy chain second complementarity-determining region (VH CDR2) and a defined heavy chain third complementarity-determining region (VH CDR3), and a light chain variable region having CDRs consisting of a defined light chain second complementarity-determining region (VL CDR2) and a defined light chain third complementarity-determining region (VL CDR3).Type: ApplicationFiled: August 20, 2014Publication date: July 14, 2016Applicants: FUJITA HEALTH UNIVERSITY, THE RESEARCH FOUNDATION FOR MICROBIAL DISEASES OF OSAKA UNIVERSITY, PERSEUS PROTEOMICS INC.Inventors: Yoshikazu KUROSAWA, Nobuko OSHIMA, Yoshinobu OKUNO, Katsuyuki MITOMO, Katsushi KOUDA
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Publication number: 20160152692Abstract: Provided is an anti-influenza virus antibody that exhibits neutralizing activity beyond the barrier of the two groups of influenza viruses categorized according to the conservativeness of hemagglutinin amino acids, a method of producing the same, and a test method for determining whether the subject carries the neutralizing antibody.Type: ApplicationFiled: February 5, 2016Publication date: June 2, 2016Inventors: Yoshikazu KUROSAWA, Yoshitaka IBA, Nobuko OHSHIMA, Yoshinobu OKUNO
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Patent number: 8975378Abstract: Provided is a human antibody having a neutralization activity against a human influenza virus. More specifically, provided is a human antibody which recognizes a highly conserved region in a human influenza A virus subtype H3N2 or a human influenza B virus and has a neutralization activity against the virus. The human antibody is a human anti-human influenza virus antibody, which has a neutralization activity against a human influenza A virus subtype H3N2 and binds to a hemagglutinin HA1 region of the human influenza A virus subtype H3N2, or which has a neutralization activity against a human influenza B virus, and includes, as a base sequence of a DNA encoding a variable region of the antibody, a sequence set forth in any one of SEQ ID NOS: 5 to 12.Type: GrantFiled: December 24, 2009Date of Patent: March 10, 2015Assignees: Osaka University, The Research Foundation for Microbial Diseases of Osaka UniversityInventors: Kazuyoshi Ikuta, Ritsuko Koketsu, Yoshinobu Okuno, Mikihiro Yunoki, Shoji Ideno, Masatoshi Oshita, Motoki Kuhara, Masatoshi Momota
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Publication number: 20150044225Abstract: Provided is a human antibody having a neutralization activity against a human influenza virus. More specifically, provided is a human antibody which recognizes a highly conserved region in a human influenza A virus subtype H3N2 or a human influenza B virus and has a neutralization activity against the virus. The human antibody is a human anti-human influenza virus antibody, which has a neutralization activity against a human influenza A virus subtype H3N2 and binds to a hemagglutinin HA1 region of the human influenza A virus subtype H3N2, or which has a neutralization activity against a human influenza B virus, and includes, as a base sequence of a DNA encoding a variable region of the antibody, a sequence set forth in any one of SEQ ID NOS: 5 to 12.Type: ApplicationFiled: October 3, 2014Publication date: February 12, 2015Inventors: Kazuyoshi Ikuta, Ritsuko Koketsu, Yoshinobu Okuno, Mikihiro Yunoki, Shoji Ideno, Masatoshi Oshita, Motoki Kuhara, Masatoshi Momota
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Publication number: 20140086927Abstract: Antibodies (Abs) play roles in protection against influenza. Neutralizing Abs either inhibit the binding of hemagglutinin (HA) to cellular receptors or prevent the conformational change of HA induced by low pH. The former Ab binds to the regions near the sialic acid-binding pocket on the globular head formed by HA1 and generally shows narrow strain specificity. The latter Ab binds to the stem region formed mainly by HA2 and shows broad strain specificity. We isolated a broadly neutralizing Ab against H3N2 viruses. X-ray analysis of the HA/Ab complex indicated that the Ab binds to the valley formed by two neighboring HA monomers at the side of the globular head. The Ab shows neutralizing activity by preventing the conformational change of HA induced at low pH.Type: ApplicationFiled: September 24, 2013Publication date: March 27, 2014Applicants: Fujita Health University, The Research Foundation for Microbial Diseases of Osaka University, Osaka UniversityInventors: Yoshikazu Kurosawa, Yoshitaka Iba, Nobuko Ohshima, Shigeyuki Yokoyama, Mikako Shirouzu, Yoshifumi Fujii, Tomomi Sumida, Kazuyoshi Ikuta, Shota Nakamura, Norihito Kawashita, Mitsuhiro Nishimura, Akifumi Yamashita, Yoshinobu Okuno, Ritsuko Kubota-Koketsu, Masahiro Okubo
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Patent number: 8298757Abstract: The present invention relates to a method for detecting an avian influenza virus by an immunological assay using an anti-influenza virus antibody being unreactive to human influenza type-A virus subtypes H1, H2 and H3 and a human influenza type-B virus and being reactive to plural subtypes of avian influenza viruses, and an immunochromatographic test tool for use in the method. According to the present invention, an avian influenza virus can be detected specifically, rapidly and in a simple manner, as distinguishing an avian influenza virus from a human influenza virus.Type: GrantFiled: August 31, 2007Date of Patent: October 30, 2012Assignees: Osaka Prefectural Government, Sysmex CorporationInventors: Kazuo Takahashi, Yoshinobu Okuno, Hiroshi Nishimura, Takeshi Imoarai, Noriyuki Saito, Tomokuni Taniguchi
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Publication number: 20120156242Abstract: Antigenic peptides are provided that can be used to induce global neutralizing antibodies, or antibodies reactive against a wide range of influenza A virus strains. The antigenic peptide can correspond to SEQ ID NO: 34 (EKEVLVLWG), SEQ ID NO: 2 (KFDKLYIWG), SEQ ID NO: 71(QEDLLVLWG), SEQ ID NO: 51 (EGRINYYWTLLEP), SEQ ID NO: 3 (PSRISIYWTIVKP), and/or SEQ ID NO: 82 (SGRMEFFWTILKP).Type: ApplicationFiled: June 16, 2010Publication date: June 21, 2012Inventors: Kazuyoshi Ikuta, Ritsuko Koketsu, Akifumi Yamashita, Norihito Kawashita, Mikihiro Yunoki, Yoshinobu Okuno, Shoji Ideno, Motoki Kuhara
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Publication number: 20120058124Abstract: Provided is an anti-influenza virus antibody that exhibits neutralizing activity beyond the barrier of the two groups of influenza viruses categorized according to the conservativeness of hemagglutinin amino acids, a method of producing the same, and a test method for determining whether the subject carries the neutralizing antibody.Type: ApplicationFiled: August 4, 2011Publication date: March 8, 2012Applicants: The Research Foundation for Microbial Diseases of Osaka University, FUJITA HEALTH UNIVERSITYInventors: Yoshikazu KUROSAWA, Yoshitaka IBA, Nobuko OHSHIMA, Yoshinobu OKUNO
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Patent number: 8101553Abstract: An antibody library is prepared by selecting a light chain variable region capable of binding to the variable region of heavy chain to reproduce an active conformation and using the same. Because of being capable of maintaining the diversity of the heavy chain variable region at a high ratio in vitro, the antibody library of the present invention is expected as enabling the acquisition of antibodies with various binding activities.Type: GrantFiled: February 22, 2001Date of Patent: January 24, 2012Assignee: Medical & Biological Laboratories Co., Ltd.Inventors: Yoshikazu Kurosawa, Yasushi Akahori, Yoshitaka Iba, Kazuhiko Morino, Midori Shinohara, Motohide Takahashi, Yoshinobu Okuno, Kimiyasu Shiraki
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Publication number: 20110319600Abstract: Provided is a human antibody having a neutralization activity against a human influenza virus. More specifically, provided is a human antibody which recognizes a highly conserved region in a human influenza A virus subtype H3N2 or a human influenza B virus and has a neutralization activity against the virus. The human antibody is a human anti-human influenza virus antibody, which has a neutralization activity against a human influenza A virus subtype H3N2 and binds to a hemagglutinin HA1 region of the human influenza A virus subtype H3N2, or which has a neutralization activity against a human influenza B virus, and includes, as a base sequence of a DNA encoding a variable region of the antibody, a sequence set forth in any one of SEQ ID NOS: 5 to 12.Type: ApplicationFiled: December 24, 2009Publication date: December 29, 2011Inventors: Kazuyoshi Ikuta, Ritsuko Koketsu, Yoshinobu Okuno, Mikihiro Yunoki, Shoji Ideno, Masatoshi Oshita, Motoki Kuhara, Masatoshi Momota
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Publication number: 20090311667Abstract: The present invention relates to a method for detecting an avian influenza virus by an immunological assay using an anti-influenza virus antibody being unreactive to human influenza type-A virus subtypes H1, H2 and H3 and a human influenza type-B virus and being reactive to plural subtypes of avian influenza viruses, and an immunochromatographic test tool for use in the method. According to the present invention, an avian influenza virus can be detected specifically, rapidly and in a simple manner, as distinguishing an avian influenza virus from a human influenza virus.Type: ApplicationFiled: August 31, 2007Publication date: December 17, 2009Applicant: Osaka Prefectural GovernmentInventors: Kazuo Takahashi, Yoshinobu Okuno, Hiroshi Nishimura, Takeshi Imoarai, Noriyuki Saito, Tomokuni Taniguchi
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Patent number: 6874230Abstract: A method of manufacturing a heat exchanger includes processes of: arranging tubes at a predetermined pitch on a set base; inserting fins into spaces each defined between the tubes; compressing the tubes and the fins in the direction of arrangement thereof; mounting hollow headers to longitudinal ends of the tubes, each header having slits engaged with a corresponding end of the tubes; and attaching end covers to both ends of the headers, each cover closing holes which open at the corresponding end of the headers.Type: GrantFiled: December 28, 2001Date of Patent: April 5, 2005Assignee: Calsonic Kansei CorporationInventors: Hiroshi Chikuma, Yoshinobu Okuno, Takahiro Nakakomi
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Publication number: 20040259153Abstract: A binding molecule that binds to a binding target substance is selected by introducing an affinity linker into the target binding substance and capturing the substance with a binding partner that binds to the affinity linker. For example, an antibody that binds to an influenza virus antigen can be easily concentrated by labeling a sugar chain present in the antigen with an affinity linker, such as biotin, and then recovering the antigen by binding them to streptavidin. The method for selecting binding molecules of the present invention is also useful in the screening of neutralizing substances.Type: ApplicationFiled: August 4, 2004Publication date: December 23, 2004Inventors: Yoshikazu Kurosawa, Yasushi Akahori, Yukari Hirono, Mai Kakita, Kazuhiro Suzuki, Yoshinobu Okuno
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Patent number: 6720409Abstract: [Object] To provide an anti-human influenza virus antibody and an immunogenic artificial polypeptide. [Constitution] An anti-human influenza virus antibody which recognizes the stem regions of haemagglutinin molecules of the H1N1 and H2N2 subtypes and has a neutralization activity but does not recognize the stem region of the H3N2 subtype and has no neutralization activity. An immunogenic artificial polypeptide having an antigenicity substantially same as the stem regions of haemagglutinin molecules. An immunogenic artificial polypeptide having an antigenicity substantially same as the stem regions of haemagglutinin molecule and lacking the globular head region of haemagglutinin molecules. [Effect] This antibody is useful in the diagnosis and treatment of influenza A virus, while these polypeptides are useful as a vaccine.Type: GrantFiled: August 1, 2001Date of Patent: April 13, 2004Assignee: Takara Shuzo Co., Ltd.Inventors: Yoshinobu Okuno, Yuji Isegawa, Fuyoko Sasao, Shigeharu Ueda
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Patent number: 6708730Abstract: A temporary fixing structure includes recesses formed with a mounting hole of a first tubular body to face each other wherein the diameter of the mounting hole extending along the recesses is smaller than that of a flange, and protrusions arranged at the head of an end portion of a second tubular body wherein a spacing between the protrusion and the flange is substantially equal to the thickness of a mounting surface of the first tubular body and wherein the protrusions are arranged inside the fist tubular body through the recesses. The second tubular body is turned with respect to the first tubular body to bring a flat portion of the flange into close contact with the mounting surface of the first tubular body.Type: GrantFiled: March 21, 2002Date of Patent: March 23, 2004Assignee: Calsonic Kansei CorporationInventors: Hiroshi Chikuma, Takahiro Nakakomi, Hirokazu Yaezawa, Yoshinobu Okuno, Katsumi Nakamura
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Patent number: 6651334Abstract: A tank of a condenser for an air conditioning system of an automotive vehicle. The tank comprises a cylindrical hollow tank main body which is formed with an arcuate cutout formed through a wall of the tank main body. A partition plate includes generally semicircular large and small diameter sections which are integral with each other to be formed into a generally disc-shape. Two projections are radially outwardly protrude respectively from opposite end portions of the generally semicircular large diameter section. During production of the tank, the partition plate is temporarily fixed to the tank main body by riveting the two projections in a state in which the partition plate has been inserted through the cutout of the tank main body, and the partition plate is brazed to the tank main body in a state in which the plate has been temporarily fixed.Type: GrantFiled: June 21, 2002Date of Patent: November 25, 2003Assignee: Calsonic Kansei CorporationInventors: Hiroshi Chikuma, Yoshinobu Okuno, Takahiro Nakakomi
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Publication number: 20030010484Abstract: A tank of a condenser for an air conditioning system of an automotive vehicle. The tank comprises a cylindrical hollow tank main body which is formed with an arcuate cutout formed through a wall of the tank main body. A partition plate includes generally semicircular large and small diameter sections which are integral with each other to be formed into a generally disc-shape. Two projections are radially outwardly protrude respectively from opposite end portions of the generally semicircular large diameter section. During production of the tank, the partition plate is temporarily fixed to the tank main body by riveting the two projections in a state in which the partition plate has been inserted through the cutout of the tank main body, and the partition plate is brazed to the tank main body in a state in which the plate has been temporarily fixed.Type: ApplicationFiled: June 21, 2002Publication date: January 16, 2003Applicant: CALSONIC KANSEI CORPORATIONInventors: Hiroshi Chikuma, Yoshinobu Okuno, Takahiro Nakakomi