Patents by Inventor Yoshio Hashizume

Yoshio Hashizume has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 5992221
    Abstract: Pipes provided with each switching valve in intermediate portions thereof for feeding sample solutions to a separation mechanism are joined by a multi-way joint, to reach the separation mechanism through a pump. When all valves are closed while driving the pump and thereafter one valve is opened, all bubbles etc. downstream the opened valve are swept and eliminated due to abrupt flow of a liquid since a portion downstream the valve has been decompressed. In switching of a sample in sample measurement, a valve of a pipe for a completely measured sample is left open while a valve of a pipe for a next sample is opened, and then the valve of the pipe for the previously completely measured sample is closed after a while. Thus, pressure fluctuation following sample switching is avoided.
    Type: Grant
    Filed: August 27, 1996
    Date of Patent: November 30, 1999
    Assignee: New Oji Paper Co., Ltd.
    Inventors: Akio Kariyone, Yoshio Hashizume, Ryuzo Hayashi
  • Patent number: 5320939
    Abstract: In a two-substrate measuring apparatus employing a first enzyme electrode for responding only to a first substrate to be measured and a second enzyme electrode for responding to both first and second substrates to be measured, in which the same enzymatic reactions are used for forming detectable substances and the substances to be finally detected by the electrodes are identical, when calculating the calibration curve for the second substrate to be measured, the output portion due to traces of the first substrate contained in the second substrate to be measured is removed, so that the second substrate is measured at a high sensitivity and high accuracy.
    Type: Grant
    Filed: September 30, 1992
    Date of Patent: June 14, 1994
    Assignee: Kanzaki Paper Manufacturing Co., Ltd.
    Inventors: Yoshio Hashizume, Ryuzo Hayashi, Akio Kariyone
  • Patent number: 5242793
    Abstract: An electrode comprising an electrically conductive base, and a selectively permeable membrane which is produced by forming a membrane from a mixed solution comprising (a) albumin, (b) at least one type of crosslinking agent and (c) chitosan. The selectively permeable membrane is useful, e.g., in conjuction with an electrode. An enzyme electrode is also provided having the selectively permeable membrane disposed on the electrode and an immobilized enzyme membrane disposed on the selectively permeable membrane. The immobilized enzyme membrane is prepared by incorporating a carboxylate into the solution containing the enzyme and an aldehyde as the crosslinking agent. The crosslinking is effected by heating or drying.
    Type: Grant
    Filed: March 7, 1990
    Date of Patent: September 7, 1993
    Assignee: Kanzaki Paper Manufacturing Co., Ltd.
    Inventors: Akio Kariyone, Ryuzo Hayashi, Yoshio Hashizume
  • Patent number: 5225321
    Abstract: In the case of measuring concentration of, for example, glucose as a first substance to be measured, and that of sucrose as a second substance to be measured, using a measuring apparatus, the apparatus is provided with a first enzyme electrode, a dilution pipeline situated at the downstream of the electrode and for diluting the substances, and a second enzyme electrode situated at the downstream of the dilution pipeline. A sample, which contains the first and second substances, is poured intermittently into a constant flow of a buffer solution. The first enzyme electrode detects the first substance. The second enzyme electrode, which comprises an immobilized enzyme catalizing the conversion of the second substance in to the first substance, detects the first substance, which is originally contained in the sample as well as is formed on the second enzyme electrode.
    Type: Grant
    Filed: July 8, 1991
    Date of Patent: July 6, 1993
    Assignee: Kanzaki Paper Mfg., Co., Ltd.
    Inventors: Ryuzo Hayashi, Akio Kariyone, Yoshio Hashizume
  • Patent number: 5221521
    Abstract: A liquid dilution system which dilutes a sample to be analyzed with a carrier to supply a diluted sample-containing liquid to an analytical measurement apparatus of flow type. The inventive dilution system features simplicity and versatility as compared to conventional automatic apparatus with robot concept, and the present system comprises: a first carrier pump for feeding a first carrier; a sample injection unit for injecting the sample into the first carrier; a main passage for flowing a liquid from the sample injection unit to a detector unit of an analytical measurement apparatus; a branching device located downstream of the sample injection unit for forming a branched passage to remove a liquid mass partially from the main passage; a confluence unit located downstream of the branching unit for confluencing a second carrier; and a second carrier pump for feeding the second carrier to the confluence unit.
    Type: Grant
    Filed: July 25, 1991
    Date of Patent: June 22, 1993
    Assignee: Kanzaki Paper Mfg. Co., Ltd.
    Inventors: Yoshio Hashizume, Akio Kariyone, Ryuzo Hayashi
  • Patent number: 5190872
    Abstract: Alcohol oxidase is immobilized by bonding an aminosilane coupling agent to a carrier, bonding a multifunctional aldehyde such as glutaraldehyde to an amino group of the aminosilane, washing the carrier to remove free multifunctional aldehyde and bonding alcohol oxidase to the multifunctional aldehyde bonded to the aminosilane. Washing to remove free multifunctional aldehyde enables immobilizing a one molecule thickness of alcohol oxidase on the carrier since free multifunctional aldehyde is not present to cross-link alcohol oxidase molecules together. A thin layer of alcohol oxidase is advantageous when assaying for alcohol since a thin layer does not retain hydrogen peroxide that can deactivate alcohol oxidase. The carrier preferably contains hydroxyl groups and is porous, and can be a silicate-containing carrier such as diatomaceous earth or fire brick.
    Type: Grant
    Filed: August 27, 1990
    Date of Patent: March 2, 1993
    Assignee: Kanzaki Paper Mfg. Co., Ltd.
    Inventors: Yoshio Hashizume, Akio Kariyone, Ryuzo Hayashi, Minako Oka
  • Patent number: 5081015
    Abstract: An enzyme electrode possessing an immobilized enzyme membrane or an immobilized enzyme layer composed by applying a solution containing alcohol oxidase and a crosslinking agent, in which the solution further contains reduced glutathione.According to the invention it is possible to immobilize alcohol oxidase stably, and an excellent immobilized enzyme electrode for alcohol measurement is obtained. Furthermore, by this invention, a highly sensitive and stable measuring method is conducted quite easily.
    Type: Grant
    Filed: August 30, 1989
    Date of Patent: January 14, 1992
    Assignee: Kanzaki Paper Mfg. Co., Ltd.
    Inventors: Ryuzo Hayashi, Akio Kariyone, Yoshio Hashizume
  • Patent number: 5081037
    Abstract: An enzyme electrode for measuring malto-oligosaccharides having a co-immobilized enzyme membrane containing glucoamylase and a glucose oxidizing enzyme such as glucose oxidase or pyranose oxidase.The glucoamylase and glucose oxidizing enzyme are immobilized at a ratio of ##EQU1## where Va is the maximum reaction rate of glucoamylase as expressed in the glucose formation rate from maltose, and Vo is the maximum reaction rate of glucose oxidizing enzyme as expressed in the hydrogen peroxide formation rate from glucose.In such enzyme electrode, malto-oligosaccharides are detected at the response value proportional to their degree of polymerization. Therefore, the malto-oligosaccharides can be measured at the glucose converted concentration.
    Type: Grant
    Filed: March 9, 1989
    Date of Patent: January 14, 1992
    Assignee: Kanzaki Paper Mfg. Co., Ltd.
    Inventors: Akio Kariyone, Yoshio Hashizume, Ryuzo Hayashi
  • Patent number: 5037527
    Abstract: A reference electrode for use in a flow type measuring apparatus incorporating an immobilized enzyme working electrode, wherein a hydrophilic gel layer is provided on a silver chloride containing layer in order to prevent flow-out of silver ions that are likely to adversely affect enzyme activity, so that the silver chloride containing layer goes into contact through the hydrophilic gel layer with an electrolytic solution containing a substance to be measured.
    Type: Grant
    Filed: August 24, 1988
    Date of Patent: August 6, 1991
    Assignee: Kanzaki Paper Mfg. Co., Ltd.
    Inventors: Ryuzo Hayashi, Kariyone Akio, Yoshio Hashizume
  • Patent number: 4299748
    Abstract: An aqueous coating composition in the form of an aqueous dispersion of acrylic copolymer prepared by polymerizing a mixture of acrylic monomers in an aqueous medium containing an electroconductive copolymer. The composition forms coatings having both heat sealing properties and antistatic properties and also possessing transparency, anti-blocking properties, slipping properties and resistance to weather and to water.
    Type: Grant
    Filed: December 14, 1978
    Date of Patent: November 10, 1981
    Assignee: Daicel Ltd.
    Inventors: Yoshio Hashizume, Masanori Itoh, Yasumasa Sakai, Akiyosi Kamita
  • Patent number: 4252697
    Abstract: An aqueous dispersion of synthetic resin mutually dissolved with a cellulose acetate alkylate is prepared by mixing water, a protective colloid and/or a surfactant, a cellulose acetate alkylate, an oil-soluble radical polymerization initiator and at least one monomer in which the cellulose acetate alkylate and the oil-soluble radical polymerization initiator are soluble, under stirring, to form an aqueous dispersion and then subjecting the monomer to radical polymerization to form a homogeneous mixture of the cellulose acetate alkylate and at least one polymer in the particles in the aqueous dispersion.
    Type: Grant
    Filed: October 11, 1979
    Date of Patent: February 24, 1981
    Assignee: Daicel, Ltd.
    Inventors: Yoshio Hashizume, Masatoshi Mikumo, Fumio Takenaka
  • Patent number: 3954903
    Abstract: A new impact-resistant ABS resin composition is disclosed comprising (1) 100 parts by weight of a graft copolymer of vinyl aromatic monomer and vinyl cyanide monomer, said copolymer being grafted onto butadiene rubber particles of a size of from 0.5 to 2.0 .mu. dispersed in the graft copolymer and (2) 1 to 10 parts by weight of a straight chain saturated polyester having an average molecular weight of from 1000 to 50000. The composition is prepared by the bulk-suspension two stage polymerization of vinyl aromatic monomer and vinyl cyanide monomer in the presence of butadiene rubber component. The polyester is incorporated during either the bulk polymerization stage or the suspension polymerization stage, or it is blended with the ABS polymer after the two stage polymerization is completed.
    Type: Grant
    Filed: October 10, 1974
    Date of Patent: May 4, 1976
    Assignee: Daicel Ltd.
    Inventors: Teizo Kudo, Yoshio Hashizume, Masanori Itoh, Makoto Shiga
  • Patent number: 3935153
    Abstract: Molding compositions having improved properties for low-pressure, low-shear molding techniques are prepared by carrying out a two-stage polymerization of an initial mixture comprising a polymerizable monomer selected from the group consisting of (1) a polymerizable aromatic monoalkenyl monomer or (2) a mixture of such aromatic monoalkenyl monomer with another polymerizable monovinyl monomer; in the presence of a butadiene rubber elastomer under bulk polymerization conditions, followed by suspension polymerization conditions; the process being characterized by the incorporation into the initial mixture of 1 to 10 wt. %, based on the polymerizable monomers, of a straight chain saturated polyester having an average molecular weight of 1,000 to 50,000.
    Type: Grant
    Filed: April 10, 1975
    Date of Patent: January 27, 1976
    Assignee: Daicel Ltd.
    Inventors: Teizo Kudo, Yoshio Hashizume, Masatoshi Mikumo, Masanori Itoh