Abstract: Disclosed is a method for administering a transgene into a fibroblast in a subject comprising: a) providing a herpes simplex virus (HSV) comprising a recombinant herpes simplex virus genome, wherein said recombinant herpes simplex virus genome comprises one or more transgenes encoding a polypeptide to be expressed in said fibroblast; and b) providing a pharmaceutically acceptable carrier; wherein said HSV has reduced cytotoxicity as compared to a wild-type herpes simplex virus.

Abstract: In one embodiment, the invention provides a herpes simplex virus (HSV) vector that does not express toxic HSV genes in non-complementing cells and which comprises a genome comprising one or more transgenes, wherein the vector is capable of expression of a transgene for at least 28 days in non-complementing cells. The invention also relates to viral stocks of the inventive vectors, compositions thereof suitable for use therapeutically or for in vitro applications, and methods relating thereto. In another aspect, the invention provides a complementing cell engineered to express ICP4 and ICP27 when the cell is infected with HSV for the production of the inventive vector.

Abstract: The invention provides a herpes simplex virus (HSV) vector that does not express toxic HSV genes in non-complementing cells and which comprises a genome comprising one or more transgenes, wherein the vector is capable of expression of a transgene for at least 28 days in non-complementing cells. The disclosed vectors include vectors having deletions in the genes ICP0, ICP4, TCP22, TCP27 and TCP47, or alternative inactivating mutations, or vectors which express one or more of these genes with modified kinetics. The invention also relates to viral stocks of the inventive vectors, compositions thereof suitable for use therapeutically or for in vitro applications, and methods relating thereto. In another aspect, the invention provides a complementing cell, in particular a U20S cell, engineered to express ICP4 and ICP27 when the cell is infected with HSV for the production of the inventive vector. Said cells are disclosed as naturally complementing ICP0.

Abstract: The invention provides a herpes simplex virus (HSV) vector that does not express toxic HSV genes in non-complementing cells and which comprises a genome comprising one or more transgenes, wherein the vector is capable of expression of a transgene for at least 28 days in non-complementing cells. The disclosed vectors include vectors having deletions in the genes ICP0, ICP4, TCP22, TCP27 and TCP47, or alternative inactivating mutations, or vectors which express one or more of these genes with modified kinetics. The invention also relates to viral stocks of the inventive vectors, compositions thereof suitable for use therapeutically or for in vitro applications, and methods relating thereto. In another aspect, the invention provides a complementing cell, in particular a U20S cell, engineered to express ICP4 and ICP27 when the cell is infected with HSV for the production of the inventive vector. Said cells are disclosed as naturally complementing ICP0.

Abstract: A hydraulic pressure source device for hydraulic boosters, comprising a hydraulic pump connected via a high-pressure oil passage to an input port of a hydraulic booster and a proportional reducing valve serving as a regulator interposed in the high-pressure oil passage, wherein the proportional reducing valve acts to reduce the pressure above a set level fed from the pump at certain reduction ratio and transmit it to the booster, the valve having a piston which is retractable beyond a valve-closing position to enlarge the volume of an output hydraulic chamber of the valve. Such retracting motion of the valve piston attenuates any impact which may be caused by kickback phenomenon occurring during operation of the booster.

Abstract: An anti-skid brake control device including a wheel cylinder having defined therein a first hydraulic chamber connected with a brake fluid line extending from the master cylinder and a second hydraulic chamber connected with a pressure controlling fluid circuit. As the brake fluid line is isolated from the pressure controlling circuit by the output member of the wheel cylinder, the brake system can operate effectively, though in a conventional manner, should oil leakage occur in the controlling circuit. On a four-wheeled vehicle, each pair of front or rear wheel cylinders can be served by a common arrangement of such pressure controlling circuit for anti-skid control.

Abstract: An anti-skid brake control device and associated method in which the device includes a wheel cylinder having defined therein a first hydraulic chamber connected with a brake fluid line extending from the master cylinder and a second hydraulic chamber connected with a pressure controlling fluid circuit. As the brake fluid line is isolated from the pressure controlling circuit by the output member of the wheel cylinder, the brake system can operate effectively, though in a conventional manner, should oil leakage occur in the controlling circuit. On a four-wheeled vehicle, each pair of front or rear wheel cylinders can be served by a common arrangement of such pressure controlling circuit for anti-skid control.

Abstract: An anti-skid brake control device and associated method in which the device includes a wheel cylinder having defined therein a first hydraulic chamber connected with a brake fluid line extending from the master cylinder and a second hydraulic chamber connected with a pressure controlling fluid circuit. As the brake fluid line is isolated from the pressure controlling circuit by the output member of the wheel cylinder, the brake system can operate effectively, though in a conventional manner, should oil leakage occur in the controlling circuit. On a four-wheeled vehicle, each pair of front or rear wheel cylinders can be served by a common arrangement of such pressure controlling circuit for anti-skid control.

Abstract: An anti-skid brake control device including a wheel cylinder having defined therein a first hydraulic chamber connected with a brake fluid line extending from the master cylinder and a second hydraulic chamber connected with a pressure controlling fluid circuit. As the brake fluid line is isolated from the pressure controlling circuit by the output member of the wheel cylinder, the brake system can operate effectively, though in a conventional manner, should oil leakage occur in the controlling circuit. On a four-wheeled vehicle, each pair of front or rear wheel cylinders can be served by a common arrangement of such pressure controlling circuit for anti-skid control.

Abstract: A device for detecting angular accelerations and decelerations of a vehicle wheel. The device comprises a first shutter member of cylindrical shape secured to a rotating member of a vehicle, which rotates with a vehicle road wheel, and having a cylindrical side wall formed therethrough with a multitude of circumferentially equispaced holes, a sensor weight mounted on said rotating member through damping means for limited angular movement relative thereto, a second shutter member of cylindrical shape secured to said weight and arranged in radially spaced coaxial relation with said first shutter member, said second shutter member having a cylindrical side wall formed therethrough with a multitude of circumferentially equispaced holes.