Abstract: Provided is an oil or fat composition of high diacylglycerol content. Even when used in cooking, the cooked food has a good external appearance without darkening, and retains its inherent flavor. Even when used in cooking after storage under light-exposed conditions, the cooked food is provided with a good external appearance and flavor. The oil or fat composition is obtained by combining, at specific contents, (A) an oil or fat of high diacylglycerol content, said oil or fat having a particular composition, (B) a plant sterol and (C) a plant sterol fatty acid ester.

Abstract: The present invention provides a method for producing a useful substance by supplying, to a fixed-bed reactor packed with an immobilized enzyme, a liquid mixture containing two liquid phases, in which the two liquid phases are allowed to flow in an identical, parallel direction, which method employs a fixed-bed reactor equipped with an insertion unit or tubes, so as to form a plurality of lumens in the fixed-bed reactor, each lumen having a cross section of a circular shape with a diameter of 100 mm or less or having a polygonal shape with a diagonal line of 100 mm or less, wherein the lumens are packed with an immobilized enzyme and the liquid mixture is supplied. In a reaction performed by passing a reaction mixture exhibiting two liquid phases through a fixed-bed reactor equipped with an immobilized enzyme, overall flow of the reaction liquid is made uniform.

Abstract: The present invention relates to a process for producing a fatty acid, which includes feeding a liquid mixture formed of two liquid phases that flow in a co-current manner into a fixed bed-type reaction column packed with immobilized lipase, and performing a hydrolysis reaction when the two liquid phases are in contact with the immobilized lipase in the fixed-bed reaction column; wherein the fixed-bed reaction column comprises partition plates so as to comprise a plurality of tube- shaped structures, wherein each tube-shaped structure has a cross -section which is rectangular, circular, oval, or polygonal in shape with at least a part being unclosed and the cross-section has a representative length of 100 mm or less.

Abstract: A process for producing fatty acids, which comprises supplying an oil phase substrate and a water phase substrate to an enzyme column packed with an immobilized enzyme, concurrently flowing them in the same direction, and hydrolyzing a fat and/or oil, wherein the oil phase substrate and the water phase substrate are alternately supplied to the enzyme column.

Abstract: The present invention relates to a process for producing a useful substance, the process includes feeding a liquid mixture formed of two liquid phases into a fixed bed-type reaction column packed with an immobilized enzyme, and allowing the liquid mixture to flow in the same direction in a co-current manner to perform a reaction. The packing thickness of the immobilized enzyme per stage of the fixed bed-type reaction column having an equivalent circular diameter of 35 mm? or larger is 10 to 200 mm. The flow of the reaction liquid inside the column can be made uniform, and as a result, reactivity and productivity is enhanced.

Abstract: The present invention relates to a process for producing a useful substance, the process including feeding a liquid mixture formed of two liquid phases into a fixed bed-type reaction column packed with an immobilized enzyme, and allowing the liquid mixture to flow in the same direction in a co-current manner to perform a reaction, wherein the packing thickness of the immobilized enzyme per stage of the fixed bed-type reaction column having an equivalent circular diameter of 35 mm? or larger is 10 to 200 mm. In the process for producing a useful substance, which is performed by feeding a liquid mixture formed of two liquid phases into a fixed bed-type reaction column packed with an immobilized enzyme, the flow of the reaction liquid inside the column can be made uniform, and as a result, reactivity and productivity can be enhanced.

Abstract: The present invention relates to a process for producing a useful substance, which includes feeding a liquid mixture formed of two liquid phases into a fixed bed-type reaction column packed with an immobilized enzyme, and performing a reaction while allowing the liquid mixture to flow in the same direction in a co-current manner, wherein the fixed bed-type reaction column has partition plates inserted in the longitudinal direction of the fixed bed-type reaction column so as to form a plurality of tube-shaped structures, wherein each tube-shaped structure has a lateral cross-section which is circular or polygonal in shape with at least a part being unclosed and has a representative length of 100 mm or less, wherein the immobilized enzyme is packed into the tube-shaped structures, and wherein the liquid mixture is fed into the tube-shaped structures.

Abstract: The present invention provides a method for producing a useful substance by supplying, to a fixed-bed reactor packed with an immobilized enzyme, a liquid mixture containing two liquid phases, in which the two liquid phases are allowed to flow in an identical, parallel direction, which method employs a fixed-bed reactor equipped with an insertion unit or tubes, so as to form a plurality of lumens in the fixed-bed reactor, each lumen having a cross section of a circular shape with a diameter of 100 mm or less or having a polygonal shape with a diagonal line of 100 mm or less, wherein the lumens are packed with an immobilized enzyme and the liquid mixture is supplied therethrough. In a reaction performed by passing a reaction mixture exhibiting two liquid phases through a fixed-bed reactor equipped with an immobilized enzyme, overall flow of the reaction liquid is made uniform, to thereby facilitate production of a useful substance in an efficient manner.

Abstract: Provided is an oil or fat composition of high diacylglycerol content. Even when used in cooking, the cooked food has a good external appearance without darkening, and retains its inherent flavor. Even when used in cooking after storage under light-exposed conditions, the cooked food is provided with a good external appearance and flavor. The oil or fat composition is obtained by combining, at specific contents, (A) an oil or fat of high diacylglycerol content, said oil or fat having a particular composition, (B) a plant sterol and (C) a plant sterol fatty acid ester.

Abstract: A process for producing fatty acids, which comprises supplying an oil phase substrate and a water phase substrate to an enzyme column packed with an immobilized enzyme, concurrently flowing them in the same direction, and hydrolyzing a fat and/or oil, wherein the oil phase substrate and the water phase substrate are alternately supplied to the enzyme column.

Abstract: Provided is a method for regenerating an immobilized enzyme for lipolysis which has been used for lipolysis, thereby exhibiting a reduced activity, which includes washing the immobilized enzyme with a solvent; controlling an equilibrium concentration of fatty acids in the solvent; removing the washed immobilized enzyme therefrom, and contacting the resulting immobilized enzyme with a fresh enzyme, wherein the fresh enzyme adsorbs onto the immobilized enzyme. According to the preferred methods of the present invention, it is possible to make effective use of the residual activity of an immobilized enzyme spent for lipolysis and regenerate the spent immobilized enzyme to have a performance similar to that before the lipolysis at a low cost using less amount of liquid waste.

Abstract: Provided is a method for regenerating an immobilized enzyme for lipolysis which has been used for lipolysis, thereby exhibiting a reduced activity, which includes washing the immobilized enzyme with a solvent; controlling an equilibrium concentration of fatty acids in the solvent; removing the washed immobilized enzyme therefrom, and contacting the resulting immobilized enzyme with a fresh enzyme, wherein the fresh enzyme adsorbs onto the immobilized enzyme. According to the preferred methods of the present invention, it is possible to make effective use of the residual activity of an immobilized enzyme spent for lipolysis and regenerate the spent immobilized enzyme to have a performance similar to that before the lipolysis at a low cost using less amount of liquid waste.