Abstract: An analysis method for hop bittering components which comprises a step of separating and analysing hop bittering components by liquid chromatography, characterized by trapping hop bittering components contained in a sample to be measured in a precolumn consisting of an ester type stationary phase for reversed phase chromatography by passing the sample through the precolumn by means of an acidic aqueous solution containing a polar organic solvent, introducing an eluent for reversed phase chromatography capable of eluting the bittering components into the precolumn, and subjecting the eluate to reversed phase liquid chromatography; and an analysis equipment which is suitable for conducting the above method.