Abstract: The present invention relates to an E. coli hisG-derived ATP-phosphoribosyltransferase variant having a reduced feedback inhibition by histidine and a strain expressing the same. The variant may maintain its activity even at a high histidine concentration, thus increasing histidine production.

Abstract: The present invention relates to a preparation method of a spheroid using human-derived cardiac stem cells and a therapeutic use for ischemic heart disease using the myocardial regeneration effect thereof. The spheroid using the cardiac stem cells provided in the present invention has excellent myocardial differentiation ability and regenerative therapeutic ability as compared to existing cardiac stem cells, and thus may be used for the treatment of ischemic heart disease such as myocardial infarction.

Abstract: Disclosed are a multilayered cell sheet of cardiac stem cells (CSCs) and a method of manufacturing the same. In particular, the present disclosure provides a method of manufacturing a multilayered cell sheet according to a single step culture procedure by using, as a three-dimensional matrix, a biodegradable natural polymer hydrogel and embedding CSCs in the hydrogel. The multilayered cell sheet of the present disclosure does not require any special device for the manufacturing, is manageable with good physicomechanical property, increases a cell engraftment rate after transplantation based on sufficient accumulation of various growth and protective factors and extracellular matrix between cells, and is also self-assembled by the cell-mediated hydrogel compaction, making nutrients transfer easy. Therefore, the multilayered cell sheet of the CSCs is expected to be usefully applicable as a therapeutic agent for myocardium regeneration.

Abstract: A method of manufacturing a multilayered cell sheet of neural crest stem cells (NCSCs), includes: (1) isolating and culturing NCSCs from peripheral nerves; (2) embedding the cultured NCSCs in a hydrogel; (3) culturing the hydrogel comprising the NCSCs embedded therein under stressed culture conditions in which a physical support is applied; and (4) culturing the resulting hydrogel of step (3) under non-stressed culture conditions in which a physical support is removed.

Abstract: Provided are a method of preparing synovium-derived mesenchymal stem cells using a synovial tissue and a hydrogel, synovium-derived mesenchymal stem cells prepared by the method, a pharmaceutical composition for treating bone or cartilage damage, the pharmaceutical composition including the synovium-derived mesenchymal stem cells, and a composition and kit for culturing mesenchymal stem cells, the composition and kit including a synovial tissue and a hydrogel. When the preparation method of the present invention is used, stem cells may be effectively extracted and obtained from synovium, and the obtained synovium-derived mesenchymal stem cells may effectively treat bone or cartilage damage, and therefore, it will be able to greatly contribute to the development of a method of treating bone or cartilage damage.

Abstract: The present invention relates to a fibrous 3-dimensional porous scaffold obtained by electro-spinning for tissue regeneration and a method for preparing the same.

Abstract: Disclosed are a multilayered cell sheet of cardiac stem cells (CSCs) and a method of manufacturing the same. In particular, the present disclosure provides a method of manufacturing a multilayered cell sheet according to a single step culture procedure by using, as a three-dimensional matrix, a biodegradable natural polymer hydrogel and embedding CSCs in the hydrogel. The multilayered cell sheet of the present disclosure does not require any special device for the manufacturing, is manageable with good physicomechanical property, increases a cell engraftment rate after transplantation based on sufficient accumulation of various growth and protective factors and extracellular matrix between cells, and is also self-assembled by the cell-mediated hydrogel compaction, making nutrients transfer easy. Therefore, the multilayered cell sheet of the CSCs is expected to be usefully applicable as a therapeutic agent for myocardium regeneration.

Abstract: The present invention relates to a preparation method of a spheroid using human-derived cardiac stem cells and a therapeutic use for ischemic heart disease using the myocardial regeneration effect thereof. The spheroid using the cardiac stem cells provided in the present invention has excellent myocardial differentiation ability and regenerative therapeutic ability as compared to existing cardiac stem cells, and thus may be used for the treatment of ischemic heart disease such as myocardial infarction.

Abstract: A method of manufacturing a multilayered cell sheet of neural crest stem cells (NCSCs), includes: (1) isolating and culturing NCSCs from peripheral nerves; (2) embedding the cultured NCSCs in a hydrogel; (3) culturing the hydrogel comprising the NCSCs embedded therein under stressed culture conditions in which a physical support is applied; and (4) culturing the resulting hydrogel of step (3) under non-stressed culture conditions in which a physical support is removed.

Abstract: A cubicle panel is disclosed wherein hangers underneath the panel are inserted into a frame and an upper end thereof is pushed upward and rotated, a magnetic catcher thereon is magnetically attached to the frame, and the panel is engaged to and/or disengaged from holes of a frame, such that cubicle assembly and disassembly are very simple, easy and less laborious because only both ends of the lower side of the panel are aligned and engaged to and/or disengaged from the holes of the frame, and the upper side of the panel is rotated and the panel is assembled and/or disassembled while both lower ends of the panel are inserted into the holes.

Abstract: A cubicle with door panels is disclosed. The cubicle including frames composed of horizontal members and vertical members erected at both sides. Each frame is coupled with a plurality of panels at front and/or rear surfaces thereof, and at least one of the panels is formed with a vertically-openable door panel. The door panel comprises wiring hanger members each formed at the front side thereof with a plurality of wiring hangers and fixed lengthwise to the vertical member; a panel plate hinged to an upper distal end of the wiring hanger member for opening upwards; and resilient means coupled to a hinge connector of the wiring hanger member and the panel plate for maintaining an openness of the panel plate when the panel plate is opened, such that additional wirings, re-wirings or checks thereto can be easily conducted, and wirings can be performed at various heights of the frame.

Abstract: A connecting apparatus for cubicle partitioning frames is disclosed. The connecting apparatus for connecting neighboring partitioning frames each formed with connecting holes comprises a connecting plate formed at one end thereof with a first hitching jaw for insertion into and passing through the connecting hole and a second hitching jaw formed at the other end thereof for not passing through the connecting hole. The apparatus further comprises a leaf spring for being insertedly hitched by the first hitching jaw of the connecting plate, and a connector housing for pressing the leaf spring to be insertedly hitched by the first hitching jaw of the connecting plate.