Abstract: Disclosed is a blood-brain barrier vascular network implemented in a microfluidic chip. The blood-brain bather vascular network structure (BBB on a chip) (in vitro blood-brain bather (BBB)-on-a chip) formed in a microfluidic chip manufactured by a present manufacturing method of the in vitro blood-brain barrier (BBB)-on-a chip may reconstruct the blood-brain barrier in a real human body more similarly due to a specific cell configuration, compared to the conventional in vitro blood-brain barrier (BBB)-on-a chip, and thus may be used as a more accurate in vitro blood-brain barrier, and may be used for the development of various cellular therapeutic agents or drugs that work in the treatment of various brain diseases.

Abstract: Disclosed is a blood-brain barrier vascular network implemented in a microfluidic chip. The blood-brain bather vascular network structure (BBB on a chip) (in vitro blood-brain bather (BBB)-on-a chip) formed in a microfluidic chip manufactured by a present manufacturing method of the in vitro blood-brain barrier (BBB)-on-a chip may reconstruct the blood-brain barrier in a real human body more similarly due to a specific cell configuration, compared to the conventional in vitro blood-brain barrier (BBB)-on-a chip, and thus may be used as a more accurate in vitro blood-brain barrier, and may be used for the development of various cellular therapeutic agents or drugs that work in the treatment of various brain diseases.

Abstract: The present invention provides a composition for treating damage of central or peripheral nerve system comprising neural precursor cell derived from subcutaneous tissue; neuron obtained by differentiating the neural precursor cell; or oligodendrocyte or Schwann cell obtained by differentiating the neural precursor cell; a use of neural precursor cell derived from subcutaneous tissue, neuron obtained by differentiating the neural precursor cell, or oligodendrocyte or Schwann cell obtained by differentiating the neural precursor cell, for the manufacture of an agent for treating damage of central or peripheral nerve system; a method for treating damage of central or peripheral nerve system which comprises administrating to a mammal a therapeutically effective amount of neural precursor cell derived from subcutaneous tissue, neurons obtained by differentiating the neural precursor cell, or oligodendrocyte or Schwann cell obtained by differentiating the neural precursor cell.

Abstract: A method of isolating epithelial cells from a human skin tissue or internal organ tissue using trypsin and ethylenediamine tetraacetic acid (EDTA) simultaneously with the application of magnetic stirring, a method of preconditioning isolated biological cells by the application of physical stimulus, i.e., strain, are provided. Epithelial cells can be isolated by the method with increased yield, colony forming efficiency (CFE), and colony size. Also, the increased percentage of stem cells in isolated cells is advantageous in therapeutic tissue implantation by autologous or allogeneic transplantation. In skin cells preconditioned by the application of strain, cell division is facilitated, and the secretion of extracellular matrix components and growth factors and the activity of matrix metalloproteinases (MMPs) are improved.

Abstract: A method of isolating epithelial cells from a human skin tissue or internal organ tissue using trypsin and ethylene-diamine tetraacetic acid (EDTA) simultaneously with the application of magnetic stirring, a method of preconditioning isolated biological cells by the application of physical stimulus, i.e., strain, are provided. Epithelial cells can be isolated by the method with increased yield, colony forming efficiency (CFE), and colony size. Also, the increased percentage of stem cells in isolated cells is advantageous in therapeutic tissue implantation by autologous or allogeneic transplantation. In skin cells preconditioned by the application of strain, cell division is facilitated, and the secretion of extracellular matrix components and growth factors and the activity of matrix metalloproteinases (MMPs) are improved.

Abstract: Disclosed are a dermal scaffold comprising alkaline pre-treated free amine-containing chitosan matrix, alkaline pre-treated free amine-containing chitosan and alkaline pre-treated collagen mixed matrix, or alkaline pre-treated free amine-containing chitosan and alkaline pre-treated collagen mixed matrix containing chitosan fabrics, which has excellent wound healing effect by constituting microenvironments suitable for migration and proliferation of fibroblasts and vascular cells surrounding the wound to be extremely useful as wound healing dressings, and a bioartificial dermis comprising the dermal scaffold and human fibroblasts, particularly useful for healing broad wound sites such as burns.

Abstract: Disclosed are a dermal scaffold comprising alkaline pre-treated free amine-containing chitosan matrix, alkaline pre-treated free amine-containing chitosan and alkaline pre-treated collagen mixed matrix, or alkaline pre-treated free amine-containing chitosan and alkaline pre-treated collagen mixed matrix containing chitosan fabrics, which has excellent wound healing effect by constituting microenvironments suitable for migration and proliferation of fibroblasts and vascular cells surrounding the wound to be extremely useful as wound healing dressings, and a bioartificial dermis comprising the dermal scaffold and human fibroblasts, particularly useful for healing broad wound sites such as burns.