Abstract: The present disclosure is to provide a method capable of producing an organic acid in high yield from an acetic acid strain using synthesis gas as a substrate. According to the present disclosure, the productivity of metabolites with C2 to C6 carbon atoms derived from synthesis gas and the selectivity of hexanoic acid production among metabolites can be improved through a first fermentation step of simultaneously providing a substrate comprising synthesis gas and sugar; and a second fermentation step of semi-mixotrophic fermentation of providing only the substrate comprising the synthesis gas. Therefore, the disclosure can contribute to sustainable chemical production using synthesis gas.

Abstract: The present disclosure relates to a composition for preparing hexanol or butanol and a method for preparing hexanol or butanol using ethanol and synthesis gas, wherein the composition according to an aspect of the present disclosure is a medium composition containing ethanol as an active ingredient, and by culturing a strain producing hexanol or butanol after inoculating with a medium containing the composition and supplying synthesis gas, hexanol or butanol can be prepared economically using inexpensive synthesis gas, and hexanol or butanol can be prepared with high efficiency by focusing the flow of a carbon source consumed in a fermentation process to the production of hexanol or butanol.

Abstract: The present invention relates to a composition for producing 2,3-butanediol by using ethanol and synthetic gas and a 2,3-butanediol production method. A composition according to an aspect of the present invention includes ethanol as an active ingredient. In the case where a 2,3-butanediol production strain is inoculated into a medium containing the composition and cultured and a synthetic gas is added thereto, 2,3-butanediol, which is a biofuel and a chemical substance, can be economically produced with ethanol serving as a substrate. In the metabolism process, the carbon flux can be concentrated on the production route of the target material, whereby the composition has an excellent effect of increasing the production efficiency of 2,3-butanediol and enhancing the productivity of 2,3-butanediol by controlling only fermentation conditions such as amounts of the synthetic gas or ethanol and stirring speeds of the medium.

Abstract: The present disclosure provides a recombinant microorganism for producing PHBV, a method for preparing the same, and a method for producing PHBV using the microorganism. The present disclosure may provide a recombinant microorganism capable of producing PHBV, which is a biodegradable plastic material with superior physical properties, directly from an inexpensive single carbon source with high efficiency without supplementation of organic acid. The present disclosure can enhance the utilization of PHA, which is expensive and has limited physical properties, and can also provide a technology more effective for industrialization using an inexpensive single carbon source. The PHBV produced according to an exemplary embodiment of the present disclosure can be used not only for general-purpose inexpensive products such as ecofriendly packing materials but also as a high-value-added medical biopolymer.

Abstract: The present specification describes a culture of a microorganism, which comprises an increased content of oleic acid, or a microbial oil comprising the same. In addition, the present specification describes a method for producing oleic acid and lipids comprising the same by culturing a microorganism. Since the present disclosure enables the production of lipids comprising oleic acid at a high concentration without genetic manipulation of a lipid-producing microorganism, it may be utilized in various industrial fields requiring oleic acid, such as foods, cosmetic materials, biofuels, etc.

Abstract: The present disclosure relates to a composition for preparing hexanol or butanol and a method for preparing hexanol or butanol using ethanol and synthesis gas, wherein the composition according to an aspect of the present disclosure is a medium composition containing ethanol as an active ingredient, and by culturing a strain producing hexanol or butanol after inoculating with a medium containing the composition and supplying synthesis gas, hexanol or butanol can be prepared economically using inexpensive synthesis gas, and hexanol or butanol can be prepared with high efficiency by focusing the flow of a carbon source consumed in a fermentation process to the production of hexanol or butanol.

Abstract: The present specification relates to a transformed yeast strain capable of simultaneously utilizing xylose and glucose as carbon sources, a preparation method thereof and a biofuel production method using the same. The transformed yeast strain transforms a wild-type yeast strain incapable of using xylose as a carbon source and simultaneously convert glucose and xylose, thereby enabling high yield production of a biofuel. The economics and sustainability of the biofuel and biomaterial production processes can be highly enhanced by providing a strain which can easily be converted to a strain capable of producing a biofuel/material in a high yield through an additional modification.

Abstract: The present disclosure relates to a Clostridium sp. JS66 strain producing metabolites having 4 to 6 carbon atoms in a high yield. The strain produces metabolites having 6 carbon atoms in a significantly high yield while reducing the production of acetic acid and ethanol as by-products.

Abstract: Disclosed herein are a yeast strain capable of utilizing xylose as a carbon source and a method for producing lipids using the same. The yeast strain is obtained by adaptively evolving a wild-type yeast strain which cannot utilize xylose as a carbon source so that it can produce high density lipids and then transforming the adaptively evolved strain to obtain the ability to metabolize xylose. Since the strain does not have the xylose metabolic pathway based on oxidoreductase, it can produce biodiesel and biomaterials based on lipid and lignocellulosic biomass at a high yield without a problem of cofactor imbalance and can greatly improve the economic feasibility and sustainability of the production processes of biodiesel and biomaterials.

Abstract: The present specification relates to a transformed yeast strain capable of simultaneously utilizing xylose and glucose as carbon sources, a preparation method thereof and a biofuel production method using the same. The transformed yeast strain transforms a wild-type yeast strain incapable of using xylose as a carbon source and simultaneously convert glucose and xylose, thereby enabling high yield production of a biofuel. The economics and sustainability of the biofuel and biomaterial production processes can be highly enhanced by providing a strain which can easily be converted to a strain capable of producing a biofuel/material in a high yield through an additional modification.

Abstract: Disclosed herein are a yeast strain capable of utilizing xylose as a carbon source and a method for producing lipids using the same. The yeast strain is obtained by adaptively evolving a wild-type yeast strain which cannot utilize xylose as a carbon source so that it can produce high density lipids and then transforming the adaptively evolved strain to obtain the ability to metabolize xylose. Since the strain does not have the xylose metabolic pathway based on oxidoreductase, it can produce biodiesel and biomaterials based on lipid and lignocellulosic biomass at a high yield without a problem of cofactor imbalance and can greatly improve the economic feasibility and sustainability of the production processes of biodiesel and biomaterials.

Abstract: The present disclosure relates to a method for separating sugars and acids with reduced energy consumption, including a step of diffusively dialyzing a first acid hydrolysate obtained by saccharifying biomass with an acid solution, thereby preparing a second acid hydrolysate wherein the concentration of the acid solution contained in the acid hydrolysate is decreased; and a step of electrolyzing the second acid hydrolysate, thereby separating sugars from the acid solution, which is advantageous in that less energy is consumed, the separated acid solution can be recycled directly without further treatment due to high concentration and loss of sugars can be minimized.

Abstract: The present disclosure relates to a Clostridium sp. JS66 strain producing metabolites having 4 to 6 carbon atoms in a high yield. The strain produces metabolites having 6 carbon atoms in a significantly high yield while reducing the production of acetic acid and ethanol as by-products.

Abstract: The present disclosure relates to a method for separating sugars and acids with reduced energy consumption, including a step of diffusively dialyzing a first acid hydrolysate obtained by saccharifying biomass with an acid solution, thereby preparing a second acid hydrolysate wherein the concentration of the acid solution contained in the acid hydrolysate is decreased; and a step of electrolyzing the second acid hydrolysate, thereby separating sugars from the acid solution, which is advantageous in that less energy is consumed, the separated acid solution can be recycled directly without further treatment due to high concentration and loss of sugars can be minimized.

Abstract: The present specification discloses a transformed Synechococcus elongatus strain which may directly produce squalene from carbon dioxide, and a method for producing squalene and a method for removing carbon dioxide, using the same. In an aspect, the strain may produce squalene using carbon dioxide as a carbon source. The Synechococcus elongatus strain is economically efficient because a high-value added squalene is produced using light and carbon dioxide present in the atmosphere as a carbon source, and the method for producing squalene is eco-friendly because the strain may be utilized to remove or reduce carbon dioxide in the atmosphere by using microorganisms. The strain of the present disclosure may produce only squalene, which is a desired target material with high purity, and has an advantage in that squalene may be continuously mass-produced.

Abstract: The present disclosure relates to a hydrolysate of a mixture of lignocellulosic biomass and seaweed biomass. By mixing seaweed biomass with lignocellulosic biomass and then preparing a hydrolysate, lignocellulosic biomass-derived acetic acid is consumed together with seaweed biomass-derived mannitol. As a result, high sugar productivity can be maintained while reducing fermentation inhibitors. Because the present disclosure can solve the problem of lignocellulosic biomass of decreased fermentation efficiency due to lignocellulose-derived fermentation inhibitors and the problem of seaweed biomass of very low productivity in spite of long fermentation time, the hydrolysate according to the present disclosure may be used to produce biofuels and biochemicals economically.

Abstract: The present disclosure discloses a transformed Synechococcus elongatus strain capable of producing biodiesel directly from carbon dioxide and a method for producing biodiesel and a method for removing carbon dioxide using the same. In an aspect, the transformed Synechococcus elongatus strain of the present disclosure can produce biodiesel in large scale using carbon dioxide as a carbon source. The Synechococcus elongatus strain is environment-friendly because it can be used to remove or reduce carbon dioxide in the atmosphere. The strain of the present disclosure is advantageous in that it can produce biodiesel in large scale because it grows faster and exhibits excellent carbon dioxide fixation capability as compared to other photosynthetic microorganisms.

Abstract: The present specification discloses a transformed Synechococcus elongatus strain which may directly produce squalene from carbon dioxide, and a method for producing squalene and a method for removing carbon dioxide, using the same. In an aspect, the strain may produce squalene using carbon dioxide as a carbon source. The Synechococcus elongatus strain is economically efficient because a high-value added squalene is produced using light and carbon dioxide present in the atmosphere as a carbon source, and the method for producing squalene is eco-friendly because the strain may be utilized to remove or reduce carbon dioxide in the atmosphere by using microorganisms. The strain of the present disclosure may produce only squalene, which is a desired target material with high purity, and has an advantage in that squalene may be continuously mass-produced.

Abstract: Disclosed herein are an expression vector capable of expressing myrcene, an Escherichia coli strain transformed with the vector and having improved capability of producing myrcene and a method for producing myrcene and a method for recycling glycerol using the same. In an aspect, the transformed Escherichia coli strain of the present disclosure can produce myrcene with high purity on a large scale using glycerol or glucose as a carbon source. Also, the Escherichia coli strain of the present disclosure is economical and environment-friendly because it can produce high value-added myrcene using waste glycerol as a carbon source. In addition, the strongly volatile myrcene can be produced and isolated at the same time.

Abstract: The present disclosure discloses a vector that can be used for both cyanobacteria and E. coli, which contains, sequentially, a pUC replication origin as a replication origin; a spectinomycin-resistant gene as a selection marker; and a promoter selected from a group consisting of a trc promoter, a tetA promoter or a modified tetA promoter, a BAD promoter and a cbbL promoter. An industrially useful substance may be produced effectively using a host cell transformed with the vector. Also, the vector may be used to insert a variety of target genes through simple combination and, as a result, various vectors can be prepared effectively.