Abstract: An application and a method of compounds inhibiting synthesis of very long chain fatty acids (VLCFAs) in preventing and controlling microbial pathogens are provided, which relate to the technical field of plant pathology and plant disease prevention and control. In particular, an application method of a compound for inhibiting the synthesis of VLCFAs in preventing and treating microbial pathogens is provided. Research results associated with the methods show that taking the synthesis of VLCFAs as the target, microbial pathogens can be inhibited by using compounds that inhibit the synthesis of VLCFAs. Therefore, the compounds inhibiting the synthesis of VLCFAs can be used in preventing and treating microbial pathogens diseases, which provides a new idea or strategy for the prevention and treatment of microbial pathogens diseases, and provides more choices for the types of drugs for the prevention and treatment of pathogenic diseases.