Abstract: A cell culture method and a cell culture system, wherein: at least a part of a culture surface of a culture container is formed of a base material, the base material having a contact angle of not more than 84°, being selected from polyethylene, polypropylene, polymethylpentene, cyclic olefin polymer, cyclic olefin copolymer, polyvinyl chloride, polyurethane, methyl polymethacrylate, polyester, polyamide, ionomer, ethylene-vinyl acetate copolymer, ethylene-vinyl alcohol copolymer, ethylene-acrylic acid copolymer, ethylene-methyl acrylate copolymer, ethylene-methacrylic acid copolymer, ethylene-methyl methacrylate copolymer and the like, and having been irradiated or hydrophilized; a medium containing laminin or a fragment thereof as a cell adhesion factor and cells are accommodated in the culture container; and adherent cells are thus cultured so that the adherent cells can be preferably cultured without coating the culture container with laminin.