Abstract: A diffuser film and a method of manufacturing the same are proposes. A colloid layer is formed on a transparent substrate. A mold plate with a raised texture is then used to roll over the colloid layer to let the surface of the colloid layer have an indented texture. Finally, a solidification process is carried out to harden the colloid layer to form a coarse layer whose surface has an indented texture. The diffuser film can thus achieve the effects of light concentration and light uniformity promotion, and can also reduce astray light rays. Moreover, using a mold plate for embossing can accomplish high reproducibility of film thickness and indented texture of the coarse layer, hence being advantageous to mass production.