Patents by Inventor Yu Ishige

Yu Ishige has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20210010970
    Abstract: There is provided an electrolyte measuring device that can decrease a liquid amount used for measurement, in which stable sealing can be provided in connecting passages of ion selective electrodes to each other with no gap, while maintaining high measurement accuracy of an existing ion selective electrode, and a residing sample liquid can be greatly decreased. In a flow-type ion selective electrode, a sealing member is used, which can be brought into intimate contact with a passage connecting unit to near a passage hole. A gap regulating member is provided to keep a gap between the electrodes constant and to prevent the sealing member from being excessively pressed. An electrode case has a structure suitable for the sealing member for allowing the alignment and holding of the sealing member.
    Type: Application
    Filed: September 28, 2020
    Publication date: January 14, 2021
    Inventors: Atsushi KISHIOKA, Yu Ishige, Tetsuyoshi Ono
  • Patent number: 10871464
    Abstract: There is provided a solid-contact ion-selective electrode having a high potential stability and potential reproducibility. The ion-selective electrode includes an ion sensitive film having a Group-1 element ion, a Group-2 element ion, a hydronium ion or an ammonium ion as a target ion, an ion occluding material layer, and a conductive electrode, wherein the ion occluding material contained in the ion occludes material layer occluding the target ion, the ion occluding material being a Prussian blue analog represented by the structure formula AaMx[M?(CN)6]y?z.kH2O. Herein, A is one type or a plurality of types of Group-1 elements, Group-2 elements, hydronium, or ammonium; M and M? are one type or a plurality of types of transition metals; M or M? includes at least one of nickel, cobalt, copper, silver, and cadmium; ? is a vacancy in a porous coordination polymer; x and y are greater than zero; and a, z, and k are numbers equal to or greater than zero.
    Type: Grant
    Filed: August 30, 2016
    Date of Patent: December 22, 2020
    Assignee: Hitachi High-Tech Corporation
    Inventors: Yu Ishige, Wolfgang Schuhmann, Stefan Klink
  • Publication number: 20200355585
    Abstract: A sample separation device to introduce a sample to be separated to an inside of the sample separation device, centrifuge the sample, and collect a desired liquid after separation, includes: a sample introducing portion introducing the sample into the sample separation device; a liquid surface defining portion connected to the sample introducing portion; a first separating portion connected to the liquid surface defining portion; a second separating portion connected to the first separating portion; a third separating portion connected to the second separating portion; and a liquid extracting portion including the third separating portion and connected to the third separating portion, in which a tubular flow path is formed by at least the first separating portion and the second separating portion, in which an opening of the sample introducing portion and an opening of the liquid extracting portion are oriented in the same direction.
    Type: Application
    Filed: November 2, 2018
    Publication date: November 12, 2020
    Inventors: Yu ISHIGE, Sakuichiro ADACHI, Takahiro ANDO
  • Patent number: 10823695
    Abstract: There is provided an electrolyte measuring device that can decrease a liquid amount used for measurement, in which stable sealing can be provided in connecting passages of ion selective electrodes to each other with no gap, while maintaining high measurement accuracy of an existing ion selective electrode, and a residing sample liquid can be greatly decreased. In a flow-type ion selective electrode, a sealing member is used, which can be brought into intimate contact with a passage connecting unit to near a passage hole. A gap regulating member is provided to keep a gap between the electrodes constant and to prevent the sealing member from being excessively pressed. An electrode case has a structure suitable for the sealing member for allowing the alignment and holding of the sealing member.
    Type: Grant
    Filed: January 22, 2015
    Date of Patent: November 3, 2020
    Assignee: Hitachi High-Technologies Corporation
    Inventors: Atsushi Kishioka, Yu Ishige, Tetsuyoshi Ono
  • Publication number: 20180298435
    Abstract: The present invention was made to provide novel methods for detecting DNA and devices therefor. Specifically, the present invention is a method of detecting DNA including the steps of: adding, to an oil, a fluorescently-labeled probe, a DNA intercalator, and a DNA solution containing a target DNA to produce droplets; performing PCR on the droplets; and measuring fluorescence from the fluorescently-labeled probe and fluorescence from the DNA intercalator, wherein the DNA solution has a concentration at which each droplet is produced so as to contain one or less target DNA molecule. In addition, the present invention is a DNA detection device including a droplet production unit, a thermal cycler unit, and a fluorescence detection unit which perform these steps.
    Type: Application
    Filed: July 5, 2016
    Publication date: October 18, 2018
    Inventors: Junko TANAKA, Yoshinobu KOHARA, Kunio HARADA, Yu ISHIGE
  • Publication number: 20180246054
    Abstract: There is provided a solid-contact ion-selective electrode having a high potential stability and potential reproducibility. The ion-selective electrode includes an ion sensitive film having a Group-1 element ion, a Group-2 element ion, a hydronium ion or an ammonium ion as a target ion, an ion occluding material layer, and a conductive electrode, wherein the ion occluding material contained in the ion occludes material layer occluding the target ion, the ion occluding material being a Prussian blue analog represented by the structure formula AaMx[M?(CN)6]y?z.kH20. Herein, A is one type or a plurality of types of Group-1 elements, Group-2 elements, hydronium, or ammonium; M and M? are one type or a plurality of types of transition metals; M or M? includes at least one of nickel, cobalt, copper, silver, and cadmium; ? is a vacancy in a porous coordination polymer; x and y are greater than zero; and a, z, and k are numbers equal to or greater than zero.
    Type: Application
    Filed: August 30, 2016
    Publication date: August 30, 2018
    Inventors: Yu ISHIGE, Wolfgang SCHUHMANN, Stefan KLINK
  • Patent number: 10018585
    Abstract: An electrolyte concentration measuring apparatus is provided with: a plurality of ion selective electrodes and one reference electrode; a sample introduction unit that introduces a sample solution to the plurality of ion selective electrodes and the reference electrode; a potential measuring unit that measures a voltage between the plurality of ion selective electrodes and the reference electrode; and a resistance measuring unit that measures a direct-current resistance of the plurality of ion selective electrodes.
    Type: Grant
    Filed: April 10, 2014
    Date of Patent: July 10, 2018
    Assignee: Hitachi High-Technologies Corporation
    Inventors: Yu Ishige, Masao Kamahori, Atsushi Kishioka, Tetsuyoshi Ono, Masafumi Miyake
  • Patent number: 9885683
    Abstract: To enhance the potential stability of a solid-electrode-type ion-selective electrode and reduce individual variations, the present invention is provided with an ion-sensitive membrane adapted to be in contact with a measurement solution, a mixture of graphite and liquid oil that is in contact with the ion-sensitive membrane, and a conductor that is in contact with the mixture of graphite and liquid oil.
    Type: Grant
    Filed: July 28, 2014
    Date of Patent: February 6, 2018
    Assignee: Hitachi High-Technologies Corporation
    Inventors: Yu Ishige, Masao Kamahori, Tetsuyoshi Ono, Yusuke Goto
  • Patent number: 9658153
    Abstract: To prevent lowering of sensitivity of a flow cell based on total reflection of light at an outer face of a glass capillary at a joint part with a pipe, the flow cell includes, at joint parts with a pipe 605 to introduce solution to a glass capillary 601 and with a pipe to discharge solution from the capillary, an inorganic material layer 602 that reflects measurement light to modify the outer face of the glass capillary as well as a reinforcement layer 711 to modify the surface thereof.
    Type: Grant
    Filed: October 30, 2012
    Date of Patent: May 23, 2017
    Assignee: Hitachi High-Technologies Corporation
    Inventors: Yusuke Goto, Masao Kamahori, Yu Ishige, Hiroshi Sasaki, Hideyuki Akiyama, Shintaro Kubo
  • Publication number: 20160334358
    Abstract: There is provided an electrolyte measuring device that can decrease a liquid amount used for measurement, in which stable sealing can be provided in connecting passages of ion selective electrodes to each other with no gap, while maintaining high measurement accuracy of an existing ion selective electrode, and a residing sample liquid can be greatly decreased. In a flow-type ion selective electrode, a sealing member is used, which can be brought into intimate contact with a passage connecting unit to near a passage hole. A gap regulating member is provided to keep a gap between the electrodes constant and to prevent the sealing member from being excessively pressed. An electrode case has a structure suitable for the sealing member for allowing the alignment and holding of the sealing member.
    Type: Application
    Filed: January 22, 2015
    Publication date: November 17, 2016
    Inventors: Atsushi KISHIOKA, Yu ISHIGE, Tetsuyoshi ONO
  • Publication number: 20160223486
    Abstract: To enhance the potential stability of a solid-electrode-type ion-selective electrode and reduce individual variations, the present invention is provided with an ion-sensitive membrane adapted to be in contact with a measurement solution, a mixture of graphite and liquid oil that is in contact with the ion-sensitive membrane, and a conductor that is in contact with the mixture of graphite and liquid oil.
    Type: Application
    Filed: July 28, 2014
    Publication date: August 4, 2016
    Inventors: Yu ISHIGE, Masao KAMAHORI, Tetsuyoshi ONO, Yusuke GOTO
  • Publication number: 20160054257
    Abstract: An electrolyte concentration measuring apparatus is provided with: a plurality of ion selective electrodes and one reference electrode; a sample introduction unit that introduces a sample solution to the plurality of ion selective electrodes and the reference electrode; a potential measuring unit that measures a voltage between the plurality of ion selective electrodes and the reference electrode; and a resistance measuring unit that measures a direct-current resistance of the plurality of ion selective electrodes.
    Type: Application
    Filed: April 10, 2014
    Publication date: February 25, 2016
    Applicant: HITACHI HIGH-TECHNOLOGIES CORPORATION
    Inventors: Yu ISHIGE, Masao KAMAHORI, Atsushi KISHIOKA, Tetsuyoshi ONO, Masafumi MIYAKE
  • Publication number: 20140332674
    Abstract: To prevent lowering of sensitivity of a flow cell based on total reflection of light at an outer face of a glass capillary at a joint part with a pipe, the flow cell includes, at joint parts with a pipe 605 to introduce solution to a glass capillary 601 and with a pipe to discharge solution from the capillary, an inorganic material layer 602 that reflects measurement light to modify the outer face of the glass capillary as well as a reinforcement layer 711 to modify the surface thereof.
    Type: Application
    Filed: October 30, 2012
    Publication date: November 13, 2014
    Inventors: Yusuke Goto, Masao Kamahori, Yu Ishige, Hiroshi Sasaki, Hideyuki Akiyama, Shintaro Kubo
  • Patent number: 8712701
    Abstract: Provided is a potentiometric sensor chip in which the positional relationship among a reference electrode, a measurement electrode, and a sample inlet which enables measurement from the start of a reaction is defined, and further provided is a method for detecting the start time of the reaction. A very small amount of sample is measured with high accuracy. The very small quantity of sample is measured by a rate assay. When a reference electrode (103) is disposed between a sample inlet (102) and a measurement electrode (104), a sample solution arrives at the reference electrode (103) earlier than at the measurement electrode (104), whereby the surface potential of the measurement electrode (104) can be measured simultaneously when the sample solution arrives at the measurement electrode (104) and dissolves a reagent and thereby a reaction starts.
    Type: Grant
    Filed: October 29, 2009
    Date of Patent: April 29, 2014
    Assignee: Hitachi, Ltd.
    Inventors: Yu Ishige, Masao Kamahori, Kuniaki Nagamine
  • Patent number: 8702933
    Abstract: A potentiometric sensor with suppressed leak current on the surface of an electrode and improved for a dynamic range and a response speed, in which a redox compound is immobilized through insulative molecules on the surface of a gold electrode, and a current between a source and drain of an insulated gate field-effect transistor along with reaction between an oxidized substance or a reduced substance produced by the reaction of a measured substance in a sample solution injector for supplying the sample solution containing the measured substance and an enzyme and a redox compound on the surface of the gold electrode, is monitored on real time to measure the change of the surface potential.
    Type: Grant
    Filed: June 14, 2012
    Date of Patent: April 22, 2014
    Assignee: Hitachi, Ltd.
    Inventors: Yu Ishige, Masao Kamahori
  • Publication number: 20130274126
    Abstract: Provided is a device that can detect cells or bacteria in units of a single cell or bacterium, and can further measure the amounts of activity of cells or bacteria or responses of the cells or bacteria to drugs in units of a single cell or bacterium. A plurality of partitioned regions each having about the same size as a cell or a bacterium is provided, and a plurality of types of electrical sensors 201 and 202 are arranged in each partitioned region.
    Type: Application
    Filed: March 14, 2013
    Publication date: October 17, 2013
    Applicant: HITACHI, LTD.
    Inventors: Yu ISHIGE, Masao KAMAHORI
  • Patent number: 8394247
    Abstract: The present invention aims to simplify the structure and a fabrication method of an ion-selective electrode in an ion concentration measuring device that measures an anion, particularly a chloride ion, in a biological component. To this end, in a potential difference measuring unit, a quaternary ammonium salt derivative serving as a ligand for an anion is immobilized to the surface of a gold electrode by using as a linker an insulative molecule forming a self-assembled monolayer. The potential difference measuring unit measures an electromotive force generated with anion binding, as an interface potential change on the surface of the gold electrode. In order to reduce the influence of adsorption of impurities on the electrode surface, a high-molecular weight polymer is physically adsorbed on the gold electrode and thus used when a biological component is measured.
    Type: Grant
    Filed: August 19, 2008
    Date of Patent: March 12, 2013
    Assignee: Hitachi High-Technologies Corporation
    Inventors: Masao Kamahori, Yu Ishige, Kotaro Yamashita, Yasuhisa Shibata, Masafumi Miyake, Kuniaki Nagamine
  • Publication number: 20130029872
    Abstract: Provided are a device, whereby cells, a bacterium or a virus can be quantified in a single unit, an assay system and an assay method. A subject to be assayed such as cells, a bacterium or a virus, which are present on a sensor, can be quantified by using a sensor equipped with multiple electrodes, said electrodes being similar in size to the subject to be assayed, detecting, concerning each electrode, the presence or absence of the subject in the vicinity of the electrode, and adding up the electrodes in which the subject is detected.
    Type: Application
    Filed: April 4, 2011
    Publication date: January 31, 2013
    Inventors: Yu Ishige, Masao Kamahori, Yusuke Goto
  • Patent number: 8323468
    Abstract: A crown ether derivative that acts as cation capturing ligand and alkanethiol having a longer carbon chain than a linker are immobilized, coexisting together, on the surface of a gold electrode, by using as the linker an insulating molecule (e.g., alkanethiol) that forms self-assembled monolayers. Electromotive force produced in association with cation coordination is measured by a potentiometer through a change in interfacial potential on the surface of the gold electrode. Further, an insulated gate field effect transistor formed on the same substrate as the gold electrode is used as the potentiometer. Furthermore, a straight-chain polymer physically adsorbed on the gold electrode is used in order to reduce the influence of the adsorption of impurities on the surface of the electrode during biological sample measurement.
    Type: Grant
    Filed: April 21, 2008
    Date of Patent: December 4, 2012
    Assignee: Hitachi-High Technologies Corporation
    Inventors: Masao Kamahori, Yu Ishige, Kotaro Yamashita, Yasuhisa Shibata, Masafumi Miyake
  • Publication number: 20120261259
    Abstract: A potentiometric sensor with suppressed leak current on the surface of an electrode and improved for a dynamic range and a response speed, in which a redox compound is immobilized through insulative molecules on the surface of a gold electrode, and a current between a source and drain of an insulated gate field-effect transistor along with reaction between an oxidized substance or a reduced substance produced by the reaction of a measured substance in a sample solution injector for supplying the sample solution containing the measured substance and an enzyme and a redox compound on the surface of the gold electrode, is monitored on real time to measure the change of the surface potential.
    Type: Application
    Filed: June 14, 2012
    Publication date: October 18, 2012
    Inventors: Yu ISHIGE, Masao KAMAHORI