Abstract: Provided is a method for constructing a gE gene-deleted pseudorabies virus (PRV) strain using an adenine base editor (ABE) and use thereof. The method includes: designing an sgRNA sequence using the ABE with a start codon of the gE gene in a PRV as a target site, ligating an enzyme-digested fragment to a double-stranded DNA fragment with sticky ends to obtain a ligation product; and transforming the ligation product into a competent cell to allow plate screening and culture, selecting a resulting positive bacterial strain to allow expanded culture, and extracting a plasmid from a resulting positive bacterial solution; and transferring the plasmid into a target cell to allow the transfection for 24 h, collecting a resulting virus liquid, and centrifuging the virus liquid to collect a resulting supernatant.

Abstract: A rate control device includes a confidence calculation circuit, a blend calculation circuit and a bit distribution circuit. The confidence calculation circuit calculates a texture confidence, a person confidence and a motion confidence according to an image. The blend calculation circuit determines, according to a mode selection signal, whether to select one of the person confidence and the motion confidence for blend calculation with the texture confidence to generate a blend parameter. The bit distribution circuit generates a bit distribution parameter according to the blend parameter.

Abstract: The present disclosure provides a kit for detecting an African swine fever virus (ASFV), and provides a corresponding single guide RNA (sgRNA) with a nucleic acid sequence shown in any one of SEQ ID NO: 1 to SEQ ID NO: 5. The kit is based on loop-mediated isothermal amplification (LAMP)-clustered regularly interspaced short palindromic repeat (CRISPR)/Cas12b and can detect the ASFV in one tube at a constant temperature. The kit only needs to set one reaction temperature, does not open its lid midway, and has high sensitivity and specificity while showing no specificity to other swine viruses. The kit exhibits high efficiency and convenience, and does not rely on a large-scale experimental equipment. Compared with the traditional fluorescence quantitative PCR methods, the kit has a greatly improved sensitivity in detecting 1 copy/?L. The kit realizes visual detection by combining with colloidal gold test strip detection.

Abstract: A device includes a first channel structure, a second channel structure, and a gate structure. The first channel structure connects a first source region and a first drain region, and includes alternating stacking first semiconductor layers and second semiconductor layers. The second semiconductor layers have a width smaller than a width of the first semiconductor layers. The second channel structure connects a second source region and a second drain region. The second channel structure includes alternating stacking third semiconductor layers and fourth semiconductor layers. The fourth semiconductor layers have a width smaller than a width of the third semiconductor layers. The gate structure wraps around the first and second channel structures.

Abstract: A device comprises source/drain regions over a substrate and spaced apart along a first direction, a first gate structure between the source/drain regions, and a first channel structure surrounded by the first gate structure. The first channel structure comprises alternately stacking first semiconductor layers and second semiconductor layers. When viewed in a cross section taken along a second direction perpendicular to the first direction, central axes of the second semiconductor layers are laterally offset from central axes of the first semiconductor layers.

Abstract: A device includes a first horizontal-gate-all-around (HGAA) transistor, a second HGAA transistor, a first vertical-gate-all-around (VGAA) transistor, and a second VGAA transistor. The first HGAA transistor and the second HGAA transistor are adjacent to each other. The first VGAA transistor is over the first HGAA transistor. The second VGAA transistor is over the second HGAA transistor. A top surface of the first VGAA transistor is substantially coplanar with a top surface of the second VGAA transistor.

Abstract: A three-level grid multi-scale finite element method for simulating groundwater flow in heterogeneous aquifers is proposed in present disclosure. The three-level grid refers to dividing the study region into coarse grid elements, then dividing each coarse grid element into medium grid elements, and finally dividing each medium grid element into fine grid elements, thereby improving the coarse-scale basis function construction method of the multi-scale finite element method. The new method of constructing a coarse-scale basis function by using the multi-scale finite element method itself instead of the finite element method is provided, constructing medium-scale basis functions on local medium grid elements, and using the medium-scale basis functions to construct a coarse-scale basis function in each coarse element, which can significantly improve the construction efficiency of the coarse-scale basis function.

Abstract: A device comprises source/drain regions over a substrate and spaced apart along a first direction, a first gate structure between the source/drain regions, and a first channel structure surrounded by the first gate structure. The first channel structure comprises alternately stacking first semiconductor layers and second semiconductor layers. When viewed in a cross section taken along a second direction perpendicular to the first direction, central axes of the second semiconductor layers are laterally offset from central axes of the first semiconductor layers.

Abstract: A device includes a first horizontal-gate-all-around (HGAA) transistor, a second HGAA transistor, a first vertical-gate-all-around (VGAA) transistor, and a second VGAA transistor. The first HGAA transistor and the second HGAA transistor are adjacent to each other. The first VGAA transistor is over the first HGAA transistor. The second VGAA transistor is over the second HGAA transistor. A top surface of the first VGAA transistor is substantially coplanar with a top surface of the second VGAA transistor.

Abstract: A method, system, and computer program product are described for quantitation of human food tastes. Such quantitation involves taste calibration of human food tasters, to constitute an initiation cohort of trained tasters who then taste and quantitatively score food items, from which a computing device determination of a normative distributized score for the food items is transmitted to user devices for quantitative guidance in selection of food and food providers, with food consumers receiving such quantitative guidance subsequently electively providing consumer scoring to enhance consistency and reliability of the food taste quantitation.

Abstract: An antenna structure applied in a wireless communication device includes a metal frame. The wireless communication device includes at least one electronic component. The metal frame includes a substrate. The substrate includes an antenna. The antenna includes a feed portion and a gap. The feed portion spans the gap. The metal frame is spaced from the electronic component. A clearance is formed between the metal frame and the electronic component.

Abstract: An antenna structure includes a metal frame, at least one feed source, and a feed portion. The metal frame includes at least one radiating portion and at least one slot. The at least one slot is disposed in the at least one radiating portion or adjacent to the at least one radiating portion. The at least one feed source and the at least one radiating portion form a first antenna. The feed portion and the at least one slot form a second antenna. The at least one feed source supplies an electric current for the first antenna, thereby exciting a first working mode and generating a radiation signal in a first frequency band. The feed portion spans the at least one slot to supply the electric current for the second antenna, thereby exciting a second working mode and generating a radiation signal in a second frequency band.

Abstract: Disclosed are detection circuit and device of TRIAC dimmer and detection method. Detection circuit comprises line voltage sampling module, peak voltage sampling module, average voltage sampling module, judgment module, bleeder module, line voltage sampling module samples line voltage, outputs sampling voltage to peak voltage sampling module and average voltage sampling module; peak voltage sampling module outputs first comparison voltage to judgment module according to sampling voltage, average voltage sampling module outputs second comparison voltage to judgment module according to sampling voltage; judgment module compares first comparison voltage with second comparison voltage and outputs control signal according to comparison result to control connection or disconnection of bleeder module; bleeder module provides discharge current for TRIAC dimmer when being connected.

Abstract: A device includes a first semiconductor fin, a second semiconductor fin, first source/drain features, second source/drain features, a first gate structure, a second gate structure, a first vertical-gate-all-around (VGAA) transistor, and a second VGAA transistor. The first semiconductor fin and the second semiconductor fin are adjacent to each other. The first source/drain features are on opposite sides of the first semiconductor fin. The second source/drain features are on opposite sides of the second semiconductor fin. The first gate structure is over the first semiconductor fin. The second gate structure is over the second semiconductor fin. The first VGAA transistor is over one of the first source/drain features. The second VGAA transistor is over one of the second source/drain features.

Abstract: An antenna structure includes a metal frame. The metal frame includes a first surface, a second surface, and a third surface. The third surface is located between the first surface and the second surface. The metal frame includes at least one antenna. The at least one antenna includes a first gap, a second gap, and a feed portion. The first gap is disposed between the first surface and the second surface. The second gap is disposed in the third surface. The feed portion is mounted on the first surface and spans the first gap. When the feed portion supplies an electric current, the electric current is coupled to the first gap and the second gap.

Abstract: A method may be performed using a server. The method may include receiving an identity authentication request associated with a user, generating authentication information based on the identity authentication request, and generating candidate information based on the authentication information such that the authentication information is a subset of the candidate information. The candidate information may be displayed at a randomly selected location. The user may provide input through a graphic input interface to select the authentication information from among the candidate information.

Abstract: Disclosed are detection circuit and device of TRIAC dimmer and detection method. Detection circuit comprises line voltage sampling module, peak voltage sampling module, average voltage sampling module, judgment module, bleeder module, line voltage sampling module samples line voltage, outputs sampling voltage to peak voltage sampling module and average voltage sampling module; peak voltage sampling module outputs first comparison voltage to judgment module according to sampling voltage, average voltage sampling module outputs second comparison voltage to judgment module according to sampling voltage; judgment module compares first comparison voltage with second comparison voltage and outputs control signal according to comparison result to control connection or disconnection of bleeder module; bleeder module provides discharge current for TRIAC dimmer when being connected.

Abstract: Systems and methods are provided for generating task reminders. The method includes identifying, using a processor, a task description by parsing digital text from a message in an application. The method further includes obtaining context information from the parsed digital text indicating an association between the task description and the application. The method additionally includes generating, using the processor, a task reminder based on the obtained context information, the task reminder including digital text indicating the association between the task description and the application.