Abstract: A primer set and a kit is used for simultaneous identification of a classical strain and a variant strain of a porcine epidemic diarrhea virus (PEDV). The primer set includes primer set I and primer set II; where primer set I comprises the nucleotide sequences set forth in SEQ ID NO: 1 to SEQ ID NO: 4; and primer set II comprises the nucleotide sequences set forth in SEQ ID NO: 5 to SEQ ID NO: 8. This approach not only helps avoid false negatives or false positives in the detection of PEDV variant strain infections, but also enables the identification between classical strain and variant strain in PEDV. This is of significant importance for understanding the prevalence of PEDV and for the prevention and control of PEDV infections.

Abstract: The present application discloses a system and a method for performing a scan chain ECO, the method includes: receiving an original netlist input by a user as first input information, the original netlist contains original scan chain information; receiving a register modification list and a scan DEF file input by the user as second input information, wherein the register modification list is configured to indicate register information to be up-chained and/or register information to be down-chained; modifying the original netlist based on the first input information and the second input information, to complete up-chaining and/or down-chaining of a list of registers in the second input information to obtain a modified scan chain netlist; and receiving the modified scan chain netlist and returning the modified scan chain netlist to the user.

Abstract: Provided is a method for constructing a gE protein-deleted pseudorabies virus (PRV) strain using an adenine base editor (ABE) and use thereof. The method includes: designing an sgRNA sequence using the ABE with a start codon of the gE gene in a PRV as a target site, ligating an enzyme-digested fragment to a double-stranded DNA fragment with sticky ends to obtain a ligation product; and transforming the ligation product into a competent cell to allow plate screening and culture, selecting a resulting positive bacterial strain to allow expanded culture, and extracting a plasmid from a resulting positive bacterial solution; and transferring the plasmid into a target cell to allow the transfection for 24 h, collecting a resulting virus liquid, and centrifuging the virus liquid to collect a resulting supernatant.

Abstract: Provided is a method for constructing a gE gene-deleted pseudorabies virus (PRV) strain using an adenine base editor (ABE) and use thereof. The method includes: designing an sgRNA sequence using the ABE with a start codon of the gE gene in a PRV as a target site, ligating an enzyme-digested fragment to a double-stranded DNA fragment with sticky ends to obtain a ligation product; and transforming the ligation product into a competent cell to allow plate screening and culture, selecting a resulting positive bacterial strain to allow expanded culture, and extracting a plasmid from a resulting positive bacterial solution; and transferring the plasmid into a target cell to allow the transfection for 24 h, collecting a resulting virus liquid, and centrifuging the virus liquid to collect a resulting supernatant.

Abstract: A rate control device includes a confidence calculation circuit, a blend calculation circuit and a bit distribution circuit. The confidence calculation circuit calculates a texture confidence, a person confidence and a motion confidence according to an image. The blend calculation circuit determines, according to a mode selection signal, whether to select one of the person confidence and the motion confidence for blend calculation with the texture confidence to generate a blend parameter. The bit distribution circuit generates a bit distribution parameter according to the blend parameter.

Abstract: The present disclosure provides a kit for detecting an African swine fever virus (ASFV), and provides a corresponding single guide RNA (sgRNA) with a nucleic acid sequence shown in any one of SEQ ID NO: 1 to SEQ ID NO: 5. The kit is based on loop-mediated isothermal amplification (LAMP)-clustered regularly interspaced short palindromic repeat (CRISPR)/Cas12b and can detect the ASFV in one tube at a constant temperature. The kit only needs to set one reaction temperature, does not open its lid midway, and has high sensitivity and specificity while showing no specificity to other swine viruses. The kit exhibits high efficiency and convenience, and does not rely on a large-scale experimental equipment. Compared with the traditional fluorescence quantitative PCR methods, the kit has a greatly improved sensitivity in detecting 1 copy/?L. The kit realizes visual detection by combining with colloidal gold test strip detection.

Abstract: A three-level grid multi-scale finite element method for simulating groundwater flow in heterogeneous aquifers is proposed in present disclosure. The three-level grid refers to dividing the study region into coarse grid elements, then dividing each coarse grid element into medium grid elements, and finally dividing each medium grid element into fine grid elements, thereby improving the coarse-scale basis function construction method of the multi-scale finite element method. The new method of constructing a coarse-scale basis function by using the multi-scale finite element method itself instead of the finite element method is provided, constructing medium-scale basis functions on local medium grid elements, and using the medium-scale basis functions to construct a coarse-scale basis function in each coarse element, which can significantly improve the construction efficiency of the coarse-scale basis function.

Abstract: A method, system, and computer program product are described for quantitation of human food tastes. Such quantitation involves taste calibration of human food tasters, to constitute an initiation cohort of trained tasters who then taste and quantitatively score food items, from which a computing device determination of a normative distributized score for the food items is transmitted to user devices for quantitative guidance in selection of food and food providers, with food consumers receiving such quantitative guidance subsequently electively providing consumer scoring to enhance consistency and reliability of the food taste quantitation.

Abstract: The disclosed embodiments relate to the design of a high-speed frequency doubler circuit. During operation, the circuit uses a transformer-based balun to convert a single-ended input signal into a differential signal, wherein the transformer-based balun includes a transformer with a primary coil, which receives the single-ended input signal, and a secondary coil, which produces the differential signal. The transformer also includes a central compensation capacitor coupled between a center tap of the secondary coil and ground, wherein the central compensation capacitor acts to suppress common-mode components in the differential signal. Next, the circuit uses a pseudo-differential amplifier to convert the differential signal into a single-ended output signal, which has double the frequency of the single-ended input signal.

Abstract: A rotary-type film folding machine comprises a feeding mechanism providing a tubular material for a folding mechanism in an axial direction, with a first driving mechanism driving the feeding mechanism to operate; a folding mechanism folding the tubular material delivered by the feeding mechanism, with a second driving mechanism driving the rotation of an outer sheath of the folding mechanism; a pressing mechanism pressing the tubular material folded by the folding mechanism, with a third driving mechanism driving the rotation of a first inner rotor for rotating the pressing mechanism and the folding mechanism; said pressing mechanism being driven by a fourth driving mechanism to move up and down; and the operations of the first, second, third and fourth driving mechanisms are controlled by a controller.

Abstract: The disclosed embodiments relate to the design of a high-speed frequency doubler circuit. During operation, the circuit uses a transformer-based balun to convert a single-ended input signal into a differential signal, wherein the transformer-based balun includes a transformer with a primary coil, which receives the single-ended input signal, and a secondary coil, which produces the differential signal. The transformer also includes a central compensation capacitor coupled between a center tap of the secondary coil and ground, wherein the central compensation capacitor acts to suppress common-mode components in the differential signal. Next, the circuit uses a pseudo-differential amplifier to convert the differential signal into a single-ended output signal, which has double the frequency of the single-ended input signal.

Abstract: A rotary-type film folding machine comprises a feeding mechanism providing a tubular material for a folding mechanism in an axial direction, with a first driving mechanism driving the feeding mechanism to operate; a folding mechanism folding the tubular material delivered by the feeding mechanism, with a second driving mechanism driving the rotation of an outer sheath of the folding mechanism; a pressing mechanism pressing the tubular material folded by the folding mechanism, with a third driving mechanism driving the rotation of a first inner rotor for rotating the pressing mechanism and the folding mechanism; said pressing mechanism being driven by a fourth driving mechanism to move up and down; and the operations of the first, second, third and fourth driving mechanisms are controlled by a controller.