Patents by Inventor Yuan-Hsin Lu

Yuan-Hsin Lu has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Polysaccharide conjugation with detoxified heat labile enterotoxin (LT) used as vaccine
    Patent number: 10624962
    Abstract: A detoxified recombinant E. coli heat-labile enterotoxin mutant, LTS61K, is employed as a carrier protein to conjugate polysaccharide. The LTS61K contains a mutated mature sub-unit A (LTA) that includes lysine at amino acid position 61 and a wild-type mature sub-unit B (LTB). Various types of bacterial capsular polysaccharide antigens were chemically conjugated with the LTS61K protein by a reductive amination reaction. The conjugated polysaccharide-LTS61K products were physically, chemically and biochemically identified as soluble form. Rabbits were immunized intramuscularly to determine the immunogenicity of conjugated vaccines by ELISA to detect anti-polysaccharide antigen IgG titers and serum bactericidal assay thereby determining the functional activity of the antibodies. Study results show that conjugated polysaccharide-LTS61K vaccines induce higher polysaccharide-specific IgG titers and greater bactericidal activity in sera than that of polysaccharide alone or polysaccharide mixed with LTS61K.
    Type: Grant
    Filed: April 29, 2011
    Date of Patent: April 21, 2020
    Assignee: DEVELOPMENT CENTER FOR BIOTECHNOLOGY
    Inventors: Yu-Shen Hsu, I-Ling Kou, Kuo-Chan Hung, Yuan-Hsin Lu, Ta-Tung Yuan
  • POLYSACCHARIDE CONJUGATION WITH DETOXIFIED E. coli HEAT LABILE ENTEROTOXIN (LT) USED AS VACCINE
    Publication number: 20110274717
    Abstract: A detoxified recombinant E. coli heat-labile enterotoxin mutant, LTS61K, is employed as a carrier protein to conjugate polysaccharide. The LTS61K contains a mutated mature sub-unit A (LTA) that includes lysine at amino acid position 61 and a wild-type mature sub-unit B (LTB). Various types of bacterial capsular polysaccharide antigens were chemically conjugated with the LTS61K protein by a reductive amination reaction. The conjugated polysaccharide-LTS61K products were physically, chemically and biochemically identified as soluble form. Rabbits were immunized intramuscularly to determine the immunogenicity of conjugated vaccines by ELISA to detect anti-polysaccharide antigen IgG titers and serum bactericidal assay thereby determining the functional activity of the antibodies. Study results show that conjugated polysaccharide-LTS61K vaccines induce higher polysaccharide-specific IgG titers and greater bactericidal activity in sera than that of polysaccharide alone or polysaccharide mixed with LTS61K.
    Type: Application
    Filed: April 29, 2011
    Publication date: November 10, 2011
    Applicant: Development Center for Biotech
    Inventors: Yu-Shen Hsu, I-Ling Kou, Kuo-Chan Hung, Yuan-Hsin Lu, Ta-Tung Yuan