Abstract: Disclosed herein is an internally fixed lipid vesicle generated from a cell that substantially preserves the membrane characteristics of the cell and methods of using the internally fixed lipid vesicle.

Abstract: A method of generating an internally fixed lipid vesicle, comprising: providing a precursor lipid vesicle that contains an aqueous interior enclosed by a lipid membrane, wherein the lipid membrane of the precursor lipid vesicle is non-permeable to a crosslinker; permeabilizing the lipid membrane transiently to generate a permeable vesicle; contacting the permeable vesicle with an inactive activatable crosslinker, whereby the inactive activatable crosslinker enters the permeable vesicle; allowing the permeable vesicle to return to a non-permeable vesicle; removing any extravesicular crosslinker; and activating the inactive activatable crosslinker to allow crosslinking to occur inside the non-permeable vesicle, whereby an internally fixed lipid vesicle is generated.

Abstract: A fluorescence lifetime imaging microscopy system comprises a microscope comprising an excitation source configured to direct an excitation energy to an imaging target, and a detector configured to measure emissions of energy from the imaging target, and a non-transitory computer-readable medium with instructions stored thereon, which perform steps comprising collecting a quantity of measured emissions of energy from the imaging target as measured data, providing a trained neural network configured to calculate fluorescent decay parameters from the quantity of measured emissions of energy, providing the data to the trained neural network, and calculating at least one fluorescence lifetime parameter with the neural network from the measured data, wherein the measured data comprises an input fluorescence decay histogram, and wherein the neural network was trained by a generative adversarial network. A method of training a neural network and a method of acquiring an image are also described.

Abstract: A method of generating an internally fixed lipid vesicle, comprising: providing a precursor lipid vesicle that contains an aqueous interior enclosed by a lipid membrane, wherein the lipid membrane of the precursor lipid vesicle is non-permeable to a crosslinker; permeabilizing the lipid membrane transiently to generate a permeable vesicle; contacting the permeable vesicle with an inactive activatable crosslinker, whereby the inactive activatable crosslinker enters the permeable vesicle; allowing the permeable vesicle to return to a non-permeable vesicle; removing any extravesicular crosslinker; and activating the inactive activatable crosslinker to allow crosslinking to occur inside the non-permeable vesicle, whereby an internally fixed lipid vesicle is generated.

Abstract: A drug carrier and a manufacturing method thereof are provided. The drug carrier includes a hydrophilic group, a hydrophobic group and an organic dye. The hydrophilic group and the hydrophobic group are connected by the organic dye. A plurality of the drug carriers are self-assemblable in a polar solvent to form a nanomicelle. The hydrophilic groups are located at the outer region of the nanomicelle, and the hydrophobic groups are located at the inner region of the nanomicelle.

Abstract: A drug carrier and a manufacturing method thereof are provided. The drug carrier includes a hydrophilic group, a hydrophobic group and an organic dye. The hydrophilic group and the hydrophobic group are connected by the organic dye. A plurality of the drug carriers are self-assemblable in a polar solvent to form a nanomicelle. The hydrophilic groups are located at the outer region of the nanomicelle, and the hydrophobic groups are located at the inner region of the nanomicelle.