Abstract: Provided is an isolated binding protein including an antigen binding domain binding to an NS1 protein, and comprising specific heavy chain CDR and light chain CDR. The binding protein can specifically identify and bind to NS1, and has relatively high sensitivity and specificity, so as to detect dengue virus. Moreover, the binding protein does not need to be produced by injecting hybridoma cells into mouse peritoneal cavity, while simplifying production, thus stabilizing antibody functionality.

Abstract: A device comprising one or more heat transfer laminates each including an electrode, a first dielectric layer on a first side of the electrode, and a second dielectric layer on a second side of the electrode; a plurality of flexible electrocaloric elements, each of the flexible electrocaloric elements including an electrocaloric material layer, a flexible electrode layer on the electrocaloric layer, one or more fixed portions each attached to one of heat transfer laminates, and a movable portion that is movable with respect to the one of the heat transfer laminates.

Abstract: Resource optimized forwarding of classified requests to different instances of a microservice is provided. The process includes obtaining classified requests to a microservice. The classified requests are classified based on different microservice resource consumption types. Further, the method includes forwarding the classified requests to instances of the microservice with different assigned resource configurations. The forwarding is based on the different microservice resource consumption types of the classified requests.

Abstract: An antibody against a novel coronavirus, and a reagent and a kit for detecting the novel coronavirus, which relate to the technical field of antibodies. The antibody against the novel coronavirus includes a heavy chain complementarity determinant region and a light chain complementarity determinant region. The antibody has good affinity for N protein of the novel coronavirus, and has good sensitivity and specificity for detecting the novel coronavirus. Antibody options are provided for detection of the novel coronavirus.

Abstract: The present disclosure relates to an HCV recombinant antigen and use of the same. Provided is an HCV recombinant antigen, which includes at least two NS3 antigens, at least one NS4 antigen, and at least one core antigen. Further provided are a nucleic acid encoding the HCV recombinant antigen, an expression vector containing the nucleic acid, a host cell containing the expression vector, a conjugate containing the HCV recombinant antigen, and a kit containing the HCV recombinant antigen, and the like.

Abstract: The application relates to an HCV recombinant antigen and a mutant thereof. The application provides an HCV recombinant antigen, the HCV recombinant antigen including at least 1 NS3 antigen, wherein at least one cysteine of the NS3 antigen in positions 1192-1517 of an HCV amino acid sequence is mutated into G and/or A, and preferably mutated into A. The application further provides a nucleic acid encoding the HCV recombinant antigen, an expression vector including the nucleic acid, a host cell including the expression vector, a conjugate including the HCV recombinant antigen, and a kit including the HCV recombinant antigen.

Abstract: A device comprising one or more heat transfer laminates each including an electrode, a first dielectric layer on a first side of the electrode, and a second dielectric layer on a second side of the electrode; a plurality of flexible electrocaloric elements, each of the flexible electrocaloric elements including an electrocaloric material layer, a flexible electrode layer on the electrocaloric layer, one or more fixed portions each attached to one of heat transfer laminates, and a movable portion that is movable with respect to the one of the heat transfer laminates.

Abstract: Disclosed is an isolated binding protein including a cardiac troponin I (cTnI) antigen binding domain, and preparation and a use and the like of the binding protein. The antigen binding domain includes at least one complementarity determining region selected from an amino acid sequence defined in this article, or has at least 80% sequence identity with the complementarity determining region of the amino acid sequence and has KD?9.96×10?8 mon affinity with cTnI. The binding protein may be used in the detection field of the cTnI protein.

Abstract: Disclosed is an isolated binding protein including a cardiac troponin I (cTnI) antigen binding domain, and preparation and a use and the like of the binding protein. The antigen binding domain includes at least one complementarity determining region selected from an amino acid sequence defined in this article, or has at least 80% sequence identity with the complementarity determining region of the amino acid sequence and has KD?7.51×10?8 mol/L affinity with cTnI. The binding protein may be used in the detection field of the cTnI.

Abstract: The present disclosure relates to a novel isolated binding protein including a N-terminal pro-brain natriuretic peptide (NT-proBNP) antigen binding domain, and the preparation method therefor. The antigen-binding domain includes at least one complementarity determining region selected from the amino acid sequences as defined in the present disclosure: or; has at least 80% sequence identity with the complementarity determining region of the following amino acid sequence and has an affinity of KD?2.26×10?8 to NT-proBNP. The binding protein may be used in the detection field of NT-proBNP protein.

Abstract: Provided is an anti-Plasmodium falciparum HRP-II antibody or an antigen-binding fragment thereof. The antibody comprises a heavy chain CDR1-3 shown in SEQ ID NO: 1-3 and a light chain CDR1-3 shown in SEQ ID NO: 4-6. Also provided are an application of the antibody and a method for preparing the antibody.

Abstract: Provided is an isolated binding protein including a Taq DNA polymerase antigen-binding domain. The antigen-binding domain includes at least one complementarity determining region selected from CDR-VH1-3 and CDR-VL1-3, or has at least 80% sequence identity with the complementarity determining region and has an affinity of KD?8.568×10?9 mol/L to the Taq DNA polymerase. The binding protein may be used in the field of molecular detection.

Abstract: Provided is an isolated binding protein including an antigen binding domain binding to an NS1 protein, and including specific heavy chain CDR and light chain CDR. The binding protein can specifically identify and bind to NS1, and has relatively high sensitivity and specificity, so as to detect dengue vims. Moreover, the binding protein does not need to be produced by injecting hybridoma cells into mouse peritoneal cavity, while simplifying production, thus stabilizing antibody functionality.

Abstract: Provided are an isolated binding protein including a pan-species-specific plasmodium lactate dehydrogenase antigen binding domain and a preparation method thereof. The antigen binding domain includes at least one complementarity determining region selected from a defined amino acid sequence, or has at least 80% of sequence identity with the complementarity determining region of the following amino acid sequence and an affinity of KD?1.5647×10?9 mol/L with a pan-species-specific plasmodium lactate dehydrogenase, and may identify the pan-species-specific plasmodium lactate dehydrogenase. The binding protein may be applied to the field of detection of plasmodium lactate dehydrogenase proteins.

Abstract: The present invention provides an isolated binding protein comprising a cTnI antigen binding domain. The antigen binding domain comprises at least one complementarity determining region selected from amino acid sequences defined herein, or has sequence identity of at least 80% to the complementarity determining region of said amino acid sequence and an affinity of KD?1.41×10?9 mol/L to cTnI. The binding protein can be used for detection of cTnI protein.

Abstract: Provided is an isolated binding protein including an antigen-binding domain that binds to NS1 protein. The isolated binding protein includes specific heavy chain CDRs and light chain CDRs. The binding protein can specifically recognize and bind to NS1, and has relatively high sensitivity and specificity, thereby achieving the detection of dengue virus. Moreover, the binding protein is not required to be produced by inducing hybridoma cells in mouse abdominal cavity, and thus it is simple in production and has more stable antibody function.

Abstract: Provided is an isolated binding protein including an antigen binding domain binding to an NS1 protein. The binding protein can identify and bind to the NS1 protein so as to detect dengue virus.

Abstract: The present disclosure relates to the field of medical diagnosis, and specifically, relates to a synthetic peptide for detecting HIV-1. The synthetic peptide is obtained by mutating the amino acid sequence set forth as SEQ ID NO: 1. The synthetic peptide can better detect anti-HIV-1 antibodies, and can better avoid potential “false negative” and “false positive” results.

Abstract: Described are shape-memory polymers that have a composite prepolymer crosslinked with a stoichiometric amount of a multifunctional crosslinker, the composite prepolymer having a branched or telechelic prepolymer having a low polydispersity reacted with a non-crystalline chain extender. Also described are methods of making shape-memory polymers by reacting a branched or telechelic prepolymer having a low polydispersity with a non-crystalline chain extender to form a composite prepolymer, and crosslinking a stoichiometric amount of a multifunctional crosslinker with the composite prepolymer, thereby forming the shape-memory polymer.

Abstract: Described are shape-memory polymers that have a branched or telechelic prepolymer having a low polydispersity crosslinked with a stoichiometric amount of a multifunctional crosslinker. Also described are methods of making shape-memory polymers by crosslinking a stoichiometric amount of a multifunctional crosslinker with a branched or telechelic prepolymer having a low polydispersity. Methods of measuring the energy storage capacity of a shape-memory polymer are also disclosed.