Abstract: Biodegradable cationic polyesters for intramuscular delivery of nucleic acids, including self-amplifying mRNA, and methods of their use for treating conditions or diseases are disclosed.

Abstract: Nanocarriers are engineered to realize the potential of RNA therapeutics. This work reports the design of pho-to-crosslinked bioreducible nanoparticles (XbNPs) for stable siRNA encapsulation in high serum conditions, shielded surface charge, efficient intracellular trafficking, and triggered cytosolic RNA release. These attributes of XbNPs leads to robust siRNA-mediated knockdown in cancer cells and potent systemic siRNA delivery to tumors in the lungs.

Abstract: Polymers and nanoparticle formulations for systemic nucleic acid delivery, including mRNA, are disclosed. A bioassay for simultaneously measuring nanoparticle cell uptake and endosomal disruption also is disclosed.

Abstract: Biodegradable particles for delivering a nucleic acid encoding gene-editing factors or a nucleic acid associated with a therapeutic protein to a cell, and compositions, methods, systems, and kits for gene editing in vivo or ex vivo or gene therapy for treating retinal eye diseases are disclosed.

Abstract: Cationic polymers having one or more anionic ligand end groups, including a new class of carboxylated branched poly(beta-amino ester)s that can self-assemble into nanoparticles for efficient intracellular delivery of different biomolecules, including a variety of proteins is disclosed.

Abstract: A corner bracket comprising of two matching components held together by two bolts wherein as the two bolts are tightened, the two matching components will be urged toward each other thereby securely gripping a planar surface that is inserted between them. Another bolt is inserted through an opening in the second component through a threaded hole in the first component and protrudes into a hole in the first component where the vertical supporting member is inserted. The bolt will securely lock the corner bracket with the planar surface to the vertical supporting member inserted through the hole in the first component. The advantage of the present invention is that the force of the grip on the planar surface can be adjusted independently of the force of the grip on the vertical supporting member. Another advantage of the present invention is that the corner bracket can be used to secure a variety of planar surfaces with a relative large range of thickness.