Abstract: The present invention relates to an improved primer editing system. and a method for performing gene editing with the system.

Abstract: The present disclosure belongs to the technical field of packings, and in particular, to a packing element, a structured packing and an application of the structured packing. The packing element includes a collection-predistribution unit, a fluid uniform distribution unit, and an opening window unit that are sequentially arranged. The fluid uniform distribution unit includes a first substrate and a plurality of orifices arranged on the first substrate. Each orifice is circular and/or semicircular. Each orifice has an equivalent diameter of 1-5 mm. The opening window unit is provided with a plurality of windows, and each window has a length proportional to 0.5 power of the fluid viscosity. Fluid forms a confined free film in the window region of the opening window unit and wall-bounded film on the wall surface region.

Abstract: Provided relates to the field of genetic engineering. In particular, a method for base editing in plant is provided. More specifically, a highly efficient A to G base editing method for a target sequence in a genome of a plant (e.g., a crop plant) by a guide RNA-directed CRISPR-adenine deaminase fusion protein and the plant and their progeny produced by the method are provided.

Abstract: The present invention belongs to the field of gene editing. In particular, the present invention relates to an improved cytosine base editing system which has a significantly reduced genome-wide off target effect and a narrow editing window.

Abstract: The present invention belongs to the field of plant genetic engineering. Specifically, the invention relates to a method for base editing in plants. More particularly, the invention relates to a method for performing efficient base editing to a target sequence in the genome of a plant (such as a crop plant) by a Cas9-cytidine deaminase fusion protein, as well as plants produced through said method and progenies thereof.

Abstract: The present invention relates to the field of plant genetic engineering. Specifically, the present invention relates to a method for targeted modification of a plant genome sequence. More specifically, the present invention relates to a method for targeted modification of a specific sequence in the plant genome into a target sequence of interest by a nuclease-reverse transcriptase fusion protein guided by a guide RNA, a genetically-modified plant produced by said method, and progenies of said plant.

Abstract: The present invention belongs to the field of plant genetic engineering. Specifically, the invention relates to a method for base editing in plants. More particularly, the invention relates to a method for performing efficient base editing to a target sequence in the genome of a plant (such as a crop plant) by a Cas9-cytidine deaminase fusion protein, as well as plants produced through said method and progenies thereof.

Abstract: Provided relates to the field of genetic engineering. In particular, a method for base editing in plant is provided. More specifically, a highly efficient A to G base editing method for a target sequence in a genome of a plant (e.g., a crop plant) by a guide RNA-directed CRISPR-adenine deaminase fusion protein and the plant and their progeny produced by the method are provided.

Abstract: The present invention belongs to the field of plant genetic engineering. Specifically, the invention relates to a method for base editing in plants. More particularly, the invention relates to a method for performing efficient base editing to a target sequence in the genome of a plant (such as a crop plant) by a Cas9-cytidine deaminase fusion protein, as well as plants produced through said method and progenies thereof.

Abstract: A PCI Express link state management system and method thereof is disclosed. The PCI Express link state management system includes an upstream device, a downstream device and a link. The upstream device outputs a configuration request to the downstream device to change a device power state of the downstream device. At the time, the link is in a first link state. The downstream device outputs a power entering signal to the upstream device and counts a time period. The link enters to a recovery state and further then return to the first link state if the downstream device does not receive a power request acknowledging signal before the time period is expired.

Abstract: A PCI Express link state management system and method thereof is disclosed. The PCI Express link power state management system includes an upstream device, a downstream device and a link. First, the upstream device outputs a configuration request to the downstream device so as to change a device power state of the downstream device. At the time, the link is in a first link state. Next, the downstream device outputs a power entering signal to the upstream device. Following that, the upstream device outputs a power request acknowledging signal to the downstream device in response to the power entering signal and a time period is counting. Finally, the downstream device re-outputs the power entering signal if the link does not enter to an electrical idle state when the time period is expired.

Abstract: In a data buffer space configuration method for requesting data from a target device via a bus, a device count of master device coupled to the bus is detected by the operating system. Then, a first data buffer space is configured to the master device if the device count is not greater than a threshold. On the other hand, a second data buffer space is configured to the master device if the device count is greater than the threshold.

Abstract: A PCI Express link state management system and method thereof is disclosed. The PCI Express link power state management system includes an upstream device, a downstream device and a link. First, the upstream device outputs a configuration request to the downstream device so as to change a device power state of the downstream device. At the time, the link is in a first link state. Next, the downstream device outputs a power entering signal to the upstream device. Following that, the upstream device outputs a power request acknowledging signal to the downstream device in response to the power entering signal and a time period is counting. Finally, the downstream device re-outputs the power entering signal if the link does not enter to an electrical idle state when the time period is expired.

Abstract: A PCI Express link state management system and method thereof is disclosed. The PCI Express link state management system includes an upstream device, a downstream device and a link. The upstream device outputs a configuration request to the downstream device to change a device power state of the downstream device. At the time, the link is in a first link state. The downstream device outputs a power entering signal to the upstream device and counts a time period. The link enters to a recovery state and further then return to the first link state.