Patents by Inventor YUANDA SONG
YUANDA SONG has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11479778Abstract: The present invention relates to the technical field of gene engineering and particularly relates to a method for constructing non-de novo synthesized Mucor circinelloides recombinant strain with high lipid yield, recombinant strain constructed by method, and application of recombinant strain. According to the present invention, a diacylglycerol acyltransferase gene (DGAT) is overexpressed in Mucor circinelloides WJ11 by a homologous recombination technology, and exogenous oil is added for fermentation, such that the non-de novo synthesized Mucor circinelloides recombinant strain with high lipid yield is constructed. Compared with the control strain Mc2075, the fat yield of the Mucor circinelloides is increased; and when the diacylglycerol acyltransferase (DGAT) is transformed into the uracil defective type of Mucor circinelloides WJ11, the fatty acid composition changes after fermentation, and the lipid content may reach 53% of dry cell weight after the fermentation condition is optimized.Type: GrantFiled: November 30, 2020Date of Patent: October 25, 2022Assignee: SHANDONG UNIVERSITY OF TECHNOLOGYInventors: Yuanda Song, Caili Sun, Huaiyuan Zhang, Wu Yang, Meiling Chen
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Patent number: 11414650Abstract: The present disclosure relates to a construction method of a Mucor circinelloides cell factory for producing dihomo-?-linolenic acid and a fermentation technology, belonging to the field of genetic engineering. In the present disclosure, ?-linolenic acid elongase gene glelo is obtained from Mortierella alpine by cloning, the gene is ligated to an integrative plasmid pMAT1552, and transformed into a Mucor circinelloides defective strain Mu402, and the gene glelo is integrated into Mucor circinelloides genome through homologous recombination, to obtain the recombinant strain Mc-glelo, and finally, the expression of the gene glelo in Mucor circinelloides is realized. The lipid content in the recombinant strain Mc-glelo is not obviously different from that in the control strain Mc1552, however, the lipid composition changes greatly, and dihomo-?-linolenic acid appears in the lipids of the recombinant strain Mc-glelo, and the content thereof reaches 5.7% of the total fatty acids.Type: GrantFiled: December 12, 2020Date of Patent: August 16, 2022Assignee: SHANDONG UNIVERSITY OF TECHNOLOGYInventors: Yuanda Song, Md. Ahsanul Kabir Khan, Junhuan Yang, Huaiyuan Zhang, Wu Yang, Qing Liu
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Patent number: 11407793Abstract: The present application discloses a dicarboxylic acid transporter and its encoding gene dit gene for increasing oil yield of Mucor circinelloides, the dit gene is cloned from the high-yield M. circinelloides WJ11, and the dit gene is transformed into M. circinelloides deficient strain Mu402, the dit gene is integrated into the genome of M. circinelloides by homologous recombination to obtain recombinant strain Mc-Dit. The total fatty acid content of the Mc-Dit strain increased by 33.76% and the intracellular lipid content may reach up to 17.67% of the dry biomass.Type: GrantFiled: November 20, 2019Date of Patent: August 9, 2022Assignee: SHANDONG UNIVERSITY OF TECHNOLOGYInventors: Yuanda Song, Junhuan Yang, Wu Yang, Shaoqi Li
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Publication number: 20220186237Abstract: The present invention relates to the technical field of gene engineering and particularly relates to a method for constructing non-de novo synthesized Mucor circinelloides recombinant strain with high lipid yield, recombinant strain constructed by method, and application of recombinant strain. According to the present invention, a diacylglycerol acyltransferase gene (DGAT) is overexpressed in Mucor circinelloides WJ11 by a homologous recombination technology, and exogenous oil is added for fermentation, such that the non-de novo synthesized Mucor circinelloides recombinant strain with high lipid yield is constructed. Compared with the control strain Mc2075, the fat yield of the Mucor circinelloides is increased; and when the diacylglycerol acyltransferase (DGAT) is transformed into the uracil defective type of Mucor circinelloides WJ11, the fatty acid composition changes after fermentation, and the lipid content may reach 53% of dry cell weight after the fermentation condition is optimized.Type: ApplicationFiled: November 30, 2020Publication date: June 16, 2022Inventors: Yuanda SONG, Caili SUN, Huaiyuan ZHANG, Wu YANG, Meiling CHEN
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Publication number: 20220177526Abstract: A C4-dicarboxylic acid transporter and its encoding gene C4mt gene can increase oil yield of Mucor circinelloides, the C4mt gene may be cloned from the high-yield M. circinelloides WJ11, and the C4mt gene is transformed into M. circinelloides deficient strain Mu402, the C4mt gene can be integrated into the genome of M. circinelloides by homologous recombination to obtain recombinant strain Mu-C4mt. The total fatty acid content of the Mu-C4mt strain can be increased by 25.30% and the intracellular lipid content may reach up to 16.34% of the dry biomass.Type: ApplicationFiled: November 20, 2019Publication date: June 9, 2022Applicant: SHANDONG UNIVERSITY OF TECHNOLOGYInventors: Yuanda SONG, Junhuan YANG, Wu YANG
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Patent number: 11345937Abstract: Provided is construction of Mucor circinelloides cell factory for producing stearidonic acid (SDA) and fermentation technology. ?15 Desaturase gene is obtained by cloning from Mortierella alpina, the gene is ligated to an integrative plasmid pMAT1552, and transformed into a Mucor circinelloides defective strain Mu402, and ?15 Desaturase gene is integrated on Mucor circinelloides genome through homologous recombination, to obtain the recombinant strain Mc-?15, finally, the expression of the ?15 Desaturase gene in Mucor circinelloides is realized. The recombinant new strain is accession number CGMCC No. 15888, and the classification name is Mucor circinelloides-D15D.Type: GrantFiled: December 12, 2020Date of Patent: May 31, 2022Assignee: SHANDONG UNIVERSITY OF TECHNOLOGYInventors: Yuanda Song, Md. Ahsanul Kabir Khan, Junhuan Yang, Yao Zhang, Wu Yang, Shaoqi Li
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Publication number: 20210292380Abstract: The present application discloses a dicarboxylic acid transporter and its encoding gene dit gene for increasing oil yield of Mucor circinelloides, the dit gene is cloned from the high-yield M. circinelloides WJ11, and the dit gene is transformed into M. circinelloides deficient strain Mu402, the dit gene is integrated into the genome of M. circinelloides by homologous recombination to obtain recombinant strain Mc-Dit. The total fatty acid content of the Mc-Dit strain increased by 33.76% and the intracellular lipid content may reach up to 17.67% of the dry biomass.Type: ApplicationFiled: November 20, 2019Publication date: September 23, 2021Applicant: SHANDONG UNIVERSITY OF TECHNOLOGYInventors: Yuanda SONG, Junhuan YANG, Wu YANG, Shaoqi LI
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Patent number: 11077162Abstract: The present disclosure provides a natural plant preparation, including extracts of the following parts of plants: roots of Salvia miltiorrhiza Bunge, Juglans regia, Aesculus hippocastanum L. and Zingiber officinale Rosc., barks of Cinnamomum cassia Presl, seeds of Sesamum indicum, Carica papaya L., Nigella damascena L. and Punica granatum L., leaves of Centaurea cyanus L., resin of Pistacia Lentiscus, drupes of Cocos nucifera L., beans of Theobroma cacao L., dried pulp and kernels of Phoenix dactylifera L., dried pulp of Olea europaea, Ficus carica Linn., Walnut and Vaccinium Spp, cloves of Allium sativum, and petals of Rosa sp. The present disclosure further provides a method for preparing a plant preparation, including treating the above parts of plants with ethanol.Type: GrantFiled: December 12, 2020Date of Patent: August 3, 2021Assignee: SHANDONG UNIVERSITY OF TECHNOLOGYInventors: Yuanda Song, Syed Ammar, Shaoqi Li, Wu Yang
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Publication number: 20210102225Abstract: Provided is construction of Mucor circinelloides cell factory for producing stearidonic acid SDA and fermentation technology thereof. ?15 Des gene is obtained by cloning from Mortierella alpina, the gene is ligated to an integrative plasmid pMAT1552, and transformed into a Mucor circinelloides defective strain Mu402, and ?15 Des is integrated on Mucor circinelloides genome through homologous recombination, to obtain the recombinant strain Mc-?15, finally, the expression of the ?15 Des gene in Mucor circinelloides is realized. The recombinant new strain was preserved in China General Microbiological Culture Collection Center on Jun. 20, 2018, and the address is NO. 1 West Beichen Road, Chaoyang District, Beijing. The center gave the biological material the preservation number CGMCC No. 15888, and the suggested classification name is Mucor circinelloides-D15D.Type: ApplicationFiled: December 12, 2020Publication date: April 8, 2021Inventors: Yuanda SONG, Md. Ahsanul Kabir KHAN, Junhuan YANG, Yao ZHANG, Wu YANG, Shaoqi LI
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Publication number: 20210102227Abstract: The present disclosure relates to a construction method of a Mucor circinelloides cell factory for producing dihomo-?-linolenic acid and a fermentation technology, belonging to the field of genetic engineering. In the present disclosure, ?-linolenic acid elongase gene glelo is obtained from Mortierella alpine by cloning, the gene is ligated to an integrative plasmid pMAT1552, and transformed into a Mucor circinelloides defective strain Mu402, and the gene glelo is integrated into Mucor circinelloides genome through homologous recombination, to obtain the recombinant strain Mc-glelo, and finally, the expression of the gene glelo in Mucor circinelloides is realized. The lipid content in the recombinant strain Mc-glelo is not obviously different from that in the control strain Mc1552, however, the lipid composition changes greatly, and dihomo-?-linolenic acid appears in the lipids of the recombinant strain Mc-glelo, and the content thereof reaches 5.7% of the total fatty acids.Type: ApplicationFiled: December 12, 2020Publication date: April 8, 2021Inventors: Yuanda SONG, Md. Ahsanul Kabir KHAN, Junhuan YANG, Huaiyuan ZHANG, Wu YANG, Qing LIU
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Publication number: 20210093688Abstract: The present disclosure provides a natural plant preparation, including extracts of the following parts of plants: roots of Salvia miltiorrhiza Bunge, Juglans regia, Aesculus hippocastanum L. and Zingiber officinale Rosc., barks of Cinnamomum cassia Presl, seeds of Sesamum indicum, Carica papaya L., Nigella damascena L. and Punica granatum L., leaves of Centaurea cyanus L., resin of Pistacia Lentiscus, drupes of Cocos nucifera L., beans of Theobroma cacao L., dried pulp and kernels of Phoenix dactylifera L., dried pulp of Olea europaea, Ficus carica Linn., Walnut and Vaccinium Spp, cloves of Allium sativum, and petals of Rosa sp. The present disclosure further provides a method for preparing a plant preparation, including treating the above parts of plants with ethanol.Type: ApplicationFiled: December 12, 2020Publication date: April 1, 2021Inventors: Yuanda SONG, Syed AMMAR, Shaoqi LI, Wu YANG
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Patent number: 9320766Abstract: The Lactobacillus plantarum CCFM8661 is tolerant to acid and lead ions in vitro which can tolerate lead ions solution with the initial concentration of 150 mg/L, and has a strong capability of binding lead ions, which can reduce the lead level in mice blood, liver, kidney and stomach, significantly improve antioxidant indicators and alleviate pathological symptoms of lead exposed mice.Type: GrantFiled: July 18, 2012Date of Patent: April 26, 2016Assignee: Jiangnan UniversityInventors: Fengwei Tian, Wei Chen, Qixiao Zhai, Hao Zhang, Jianxin Zhao, Gang Wang, Yuanda Song, Qiuxiang Zhang, Xiaoming Liu, Min Guo, Daming Fan
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Patent number: 9315836Abstract: The present invention provides novel fatty acid desaturases genes used for synthesis of polyunsaturated fatty acids, especially ?3 desaturases (FADS15). The present invention also provides nucleic acid sequence coding the above-described desaturases, expression vector of the above-described desaturases and recombinant microorganism expressing above-described desaturases.Type: GrantFiled: July 4, 2014Date of Patent: April 19, 2016Assignee: University of JiangnanInventors: Haiqin Chen, Zhennan Gu, Hao Zhang, Wei Chen, Yuanda Song, Fengwei Tian, Jianxin Zhao, Yongquan Chen
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Patent number: 9115339Abstract: The present invention provides novel fatty acid desaturases genes used for synthesis of polyunsaturated fatty acids, especially delta-9 desaturases (FADS9-I). The present invention also provides nucleic acid sequence coding the above-described desaturases, expression vector of the above-described desaturases and recombinant microorganism expressing above-described desaturases.Type: GrantFiled: August 22, 2013Date of Patent: August 25, 2015Assignee: UNIVERSITY OF JIANGNANInventors: Wei Chen, Haiqun Chen, Zhennan Gu, Hao Zhang, Yuanda Song, Jianxin Zhao, Fengwei Tian, Yongquan Chen
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Patent number: 8962276Abstract: The present invention provides novel fatty acid desaturases genes used for synthesis of polyunsaturated fatty acids, especially omega-3 desaturases (FADS15). The present invention also provides nucleic acid sequence coding the above-described desaturases, expression vector of the above-described desaturases and recombinant microorganism expressing above-described desaturases.Type: GrantFiled: October 6, 2012Date of Patent: February 24, 2015Assignee: University of JiangnanInventors: Haiqin Chen, Zhennan Gu, Hao Zhang, Wei Chen, Yuanda Song, Fengwei Tian, Jianxin Zhao, Yongquan Chen
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Publication number: 20140369981Abstract: The Lactobacillus plantarum CCFM8661 is tolerant to acid and lead ions in vitro which can tolerate lead ions solution with the initial concentration of 150 mg/L, and has a strong capability of binding lead ions, which can reduce the lead level in mice blood, liver, kidney and stomach, significantly improve antioxidant indicators and alleviate pathological symptoms of lead exposed mice.Type: ApplicationFiled: July 18, 2012Publication date: December 18, 2014Applicant: Jiangnan UniversityInventors: Fengwei Tian, Wei Chen, Qixiao Zhai, Hao Zhang, Jianxin Zhao, Gang Wang, Yuanda Song, Qiuxiang Zhang, Xiaoming Liu, Min Guo, Daming Fan
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Publication number: 20140363860Abstract: The present invention provides novel fatty acid desaturases genes used for synthesis of polyunsaturated fatty acids, especially ?3 desaturases (FADS15). The present invention also provides nucleic acid sequence coding the above-described desaturases, expression vector of the above-described desaturases and recombinant microorganism expressing above-described desaturases.Type: ApplicationFiled: July 4, 2014Publication date: December 11, 2014Applicant: University of JiangnanInventors: Haiqin Chen, Zhennan Gu, Hao Zhang, Wei Chen, Yuanda Song, Fengwei Tian, Jianxin Zhao, Yongquan Chen
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Publication number: 20140099683Abstract: The present invention provides novel fatty acid desaturases genes used for synthesis of polyunsaturated fatty acids, especially omega-3 desaturases (FADS15). The present invention also provides nucleic acid sequence coding the above-described desaturases, expression vector of the above-described desaturases and recombinant microorganism expressing above-described desaturases.Type: ApplicationFiled: October 6, 2012Publication date: April 10, 2014Applicant: UNIVERSITY OF JIANGNANInventors: Haiqin Chen, Zhennan Gu, Hao Zhang, Wei Chen, Yuanda Song, Fengwei Tian, Jianxin Zhao, Yongquan Chen
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Publication number: 20140065691Abstract: The present invention provides novel fatty acid desaturases genes used for synthesis of polyunsaturated fatty acids, especially delta-9 desaturases (FADS9-I). The present invention also provides nucleic acid sequence coding the above-described desaturases, expression vector of the above-described desaturases and recombinant microorganism expressing above-described desaturases.Type: ApplicationFiled: August 22, 2013Publication date: March 6, 2014Applicant: UNIVERSITY OF JIANGNANInventors: WEI CHEN, HAIQUN CHEN, ZHENNAN GU, HAO ZHANG, YUANDA SONG, JIANXIN ZHAO, FENGWEI TIAN, YONGQUAN CHEN