Abstract: A genome-specific gene sequence fragment of female Acipenser Dabryanus and a method for identifying the sex of Acipenser Dabryanus are disclosed. The gene sequence fragment of Acipenser Dabryanus is used to identify the sex of Acipenser Dabryanus. The method includes: performing PCR amplification on the DNA of the Acipenser Dabryanus to be tested, and judging that the Acipenser Dabryanus to be tested is female when a resultant product from the amplification shows 3 bands on an agarose gel and the Acipenser Dabryanus to be tested is male when a resultant product from the amplification shows 2 bands on an agarose gel, alternatively, judging that the Acipenser Dabryanus to be tested is female when a resultant fragment from the amplification has a 1402 bp band and it is male if there is no 1402 bp band. The gene sequence fragment is used to quickly and efficiently identify the sex of Acipenser Dabryanus.