Abstract: The invention provides a force stimulation loading device and a working method thereof, the force stimulation loading device comprises: a containing body, a stretching mechanism and a twisting mechanism; the containing body is suitable for containing a gel encapsulating cardiomyocytes, and is made of a non-rigid material; the stretching mechanism is suitable for stretching or squeezing the containing body from opposite sides of the containing body to apply a stretching or squeezing force to the gel; the twisting mechanism is suitable for twisting the containing body to apply a twisting stress to the gel; the force stimulation loading device of the present invention is capable of simultaneously applying the stretching or squeezing force and the twisting stress to the gel encapsulating the cardiomyocytes through the stretching mechanism and the twisting mechanism, that is, applying the stretching or squeezing force and the twisting stress to the cardiomyocytes at the same time.