Abstract: A transcription regulation system based on CRISPRi and CRISPRa, and an establishment method therefor and the use thereof. The system is a new transcription regulation system which has high expression intensity, a low leakage level and is flexible and programmable. Provided is a method for achieving high-intensity and low-leakage expression of a gene by means of the transcription regulation system, wherein a high-intensity transcription level is achieved while suppressing background expression by means of synergistic regulation on downstream signaling effect devices by means of CRISPRi and CRISPRa dev ices. The new transcription regulation system can obtain, by means of loading different input promoters, a new expression system for responding to a specific signal, and has application value in the development and establishment of an efficient heterologous protein expression platform and a microbial cell factory.

Abstract: The present disclosure provides a visual navigation method and system for mobile devices based on QR code signposts. The visual navigation method for movement includes: autonomously planning an optimal route according to a destination specified by a user, performing positioning in an image captured by a trolley camera and recognizing signpost information, and adjusting a pose according to the signpost information and going to a signpost in the path. In this process, positioning is performed in real time and route planning is updated, and a user may change the destination at any time in a trolley navigation process according to a robust anomaly recovery decision. The method disclosed by the present disclosure not only proposes an indoor navigation solution that does not rely on a GPS signal and strong computation resources, but also can effectively meet an indoor scheduling scenario in which a path needs to be planned intelligently.

Abstract: Provided is a method of eliminating the dependence of methanol induced promoter on a single methanol carbon source for expressing foreign polypeptide. The method comprises activating the expression of the promoter requiring methanol induction by increasing the expression quantity of Mit1 polypeptide in cells of methylotrophic yeast, so that the promoter originally depending on methanol induction no longer depends on single methanol and can also express foreign polypeptide.

Abstract: Provided is a method of eliminating the dependence of methanol induced promoter on a single methanol carbon source for expressing foreign polypeptide. The method comprises activating the expression of the promoter requiring methanol induction by increasing the expression quantity of Mit1 polypeptide in cells of methylotrophic yeast, so that the promoter originally depending on methanol induction no longer depends on single methanol and can also express foreign polypeptide.

Abstract: A novel polyvalent attenuated live bacterial vaccine for preventing and curing vibriosis of cultivated fish is provided. The vaccine mainly comprises attenuated deletion strain of Vibrio anguillarum without marker gene, which has significant low toxicity, but remains immunogenicity against wild type strain of V. anguillarum, as compared with wild type strain MVM425. Moreover, the vaccine strain has excellent cross immunoprotection against Vibrio alginolyticus. The attenuated live vaccine made from the vaccine strain is effective to prevent and cure vibriosis of fish resulted from wild type strain of V. anguillarum and V. alginolyticus.

Abstract: A novel polyvalent attenuated live bacterial vaccine for preventing and curing vibriosis of cultivated fish is provided. The vaccine mainly comprises attenuated deletion strain of Vibrio anguillarum without marker gene, which has significant low toxicity, but remains immunogenicity against wild type strain of V. anguillarum, as compared with wild type strain MVM425. Moreover, the vaccine strain has excellent cross immunoprotection against Vibrio alginolyticus. The attenuated live vaccine made from the vaccine strain is effective to prevent and cure vibriosis of fish resulted from wild type strain of V. anguillarum and V. alginolyticus.

Abstract: A cell-detaching reactor is provided to prepare single cell suspension for inoculation of anchorage-dependent cells between the scaled-up bioreactors, which is especially useful in a commercial-scale process. The cell-detaching reactor of the invention comprises a trypsinizing zone and a separating zone, which are separated by a screen with mesh size between the diameters of the cells and the carriers.