Abstract: The present disclosure relates to a method and apparatus for pushing subscription data in Internet of things. The method includes: parsing a subscription condition set into a first attribute set in response to receiving the subscription condition set; acquiring device data; parsing the device data into a second attribute set; performing Bloom filtering on attribute elements in the second attribute set one by one in a predetermined order by taking an attribute element set in the first attribute set as a filter condition; stopping the Bloom filtering on the other attribute elements in the second attribute set in the case that a Bloom filtering result of one attribute element indicates an occurrence of mismatch; and returning to the step of acquiring the device data.

Abstract: The present disclosure relates to a method and apparatus for pushing subscription data in Internet of things. The method includes: parsing a subscription condition set into a first attribute set in response to receiving the subscription condition set; acquiring device data; parsing the device data into a second attribute set; performing Bloom filtering on attribute elements in the second attribute set one by one in a predetermined order by taking an attribute element set in the first attribute set as a filter condition; stopping the Bloom filtering on the other attribute elements in the second attribute set in the case that a Bloom filtering result of one attribute element indicates an occurrence of mismatch; and returning to the step of acquiring the device data.

Abstract: The present disclosure relates to the field of synthesis of mercapto compounds, and in particular, to a preparation method of 3-mercaptopropionic acid. The preparation method provided by the present disclosure comprises the steps of: mixing sodium acrylate with sodium hydrosulfide or sodium sulfide for reacting; adding sodium sulfide (or adding sodium sulfide and sulfur powder) for further reacting; crystallizing, and acidifying with an acid to obtain a solution of 3-mercaptopropionic acid. According to the preparation method provided by the present disclosure, the raw materials are inexpensive, the product 3-mercaptopropionic acid can be obtained with a high yield and high purity, moreover, and solid waste and solvent(s) can be separated and recovered; sodium salt can be used as an industrial product after crystallization; the solvent(s) can be recovered and recycled by distillation; and disposal of three wastes is simple.

Abstract: The present invention provides a fused NHase with improved specific activity and stability, which relates to the field of genetic engineering. This invention provides a method of overexpressing a fused NHase in E. coli and producing a mutant NHase with improved stability and product tolerance. The invention provides a simple, efficient and safe method of making mutant NHase, and can produce a large amount of soluble NHases in a short period. The present invention makes a contribution to large-scale industrial production and further theoretical study of NHases.

Abstract: The present invention provides a fused NHase with improved specific activity and stability, which relates to the field of genetic engineering. This invention provides a method of overexpressing a fused NHase in E. coli and producing a mutant NHase with improved the stability and product tolerance. The invention provides a simple, efficient and safe method of making mutant NHase, and can produce a large amount of soluble NHases in a short period. The present invention makes a contribution to large-scale industrial production and further theoretical study of NHases.

Abstract: The present invention provides a fused NHase with improved specific activity and stability, which relates to the field of genetic engineering. This invention provides a method of overexpressing a fused NHase in E. coli and producing a mutant NHase with improved stability and product tolerance. The invention provides a simple, efficient and safe method of making mutant NHase, and can produce a large amount of soluble NHases in a short period. The present invention makes a contribution to large-scale industrial production and further theoretical study of NHases.

Abstract: The present invention provides a fused NHase with improved specific activity and stability, which relates to the field of genetic engineering. This invention provides a method of overexpressing a fused NHase in E. coli and producing a mutant NHase with improved the stability and product tolerance. The invention provides a simple, efficient and safe method of making mutant NHase, and can produce a large amount of soluble NHases in a short period. The present invention makes a contribution to large-scale industrial production and further theoretical study of NHases.