Abstract: Provided is a nucleic acid sample-contained container including; a first nucleic acid molecule including an intended base sequence and a base sequence for detection different from the intended base sequence; and a second nucleic acid molecule free of the intended base sequence but including the base sequence for detection, wherein the nucleic acid sample-contained container includes the first nucleic acid molecule in a predetermined number. In a preferable mode, the copy number of the intended base sequence is less than 1,000, and the coefficient of variation (CV value) for the copy number is lower than 20%. In a more preferable mode, the nucleic acid molecules are artificially synthesized nucleic acid molecules. In a yet more preferable mode, the first nucleic acid molecule includes the intended base sequence in a plural number in the same molecule.

Abstract: There is provided a production method for microglia, including a step of introducing, into pluripotent stem cells, a nucleic acid including an open reading frame of a transcription factor that increases expression of a Complement C3 (C3) gene. In the production method, the transcription factor is selected from the group consisting of SPI1, CEBPA, PTAFR, FLI1, MEF2C, EGR2, RUNX1, TNF, CEBPB, IKZF1, KLF6, NFIC, ELF4, PAX8, PRDM1, MEF2A, and NR4A3.

Abstract: In one embodiment, provided are a method for analyzing at least one nucleic acid that can conveniently and highly accurately analyze even a very small number of analyte at least one nucleic acid. In one embodiment, the present invention relates to a method for analyzing at least one nucleic acid, comprising: a library preparation step of preparing a library comprising at least one standard nucleic acid of specific copy number(s) and at least one analyte nucleic acid in a same system; a calibration curve data generation step of generating calibration curve data based on the copy number(s) of the at least one standard nucleic acid of specific copy number(s); and an analyte nucleic acid analysis step of identifying at least one nucleotide sequence of the analyte nucleic acid while identifying the number(s) of the at least one nucleotide sequence of the at least one analyte nucleic acid using the calibration curve data.

Abstract: Provided is a device including at least one well and an amplifiable reagent contained in a specific copy number in the at least one well. In a preferable mode, the device includes information on the specific copy number of the amplifiable reagent. In a more preferable mode, the device includes information on uncertainty as the information on the specific copy number, and the information on uncertainty includes a coefficient of variation CV of the amplifiable reagent, and the coefficient of variation CV satisfies a relational expression: CV<1/Vx, where x represents an average specific copy number of the amplifiable reagent. In a particularly preferable mode, the device includes a plurality of wells in which the amplifiable reagent is contained, and the amplifiable reagent is contained in each of the wells in the same specific copy number. The invention may use microscopes to verify the number of cells comprising the amplifiable reagent, i.e. nucleic acid, in each of the wells.

Abstract: Provided is a device including: at least one well in which an amplifiable reagent is contained in a specific copy number of less than 100; and at least one well in which the amplifiable reagent is contained in a specific copy number of 100 or greater, wherein for the at least one well in which the specific copy number of the amplifiable reagent is less than 100, a formula: CV<1/?x is established, where CV represents a coefficient of variation for the specific copy number and x represents average specific copy number of the amplifiable reagent.

Abstract: Provided is a detection determining method used in detection of a testing target in a sample by amplification of the testing target and an amplifiable reagent, wherein the amplifiable reagent is provided in a number of 200 or less, the detection determining method including a determining step of determining that the testing target is present and a detection result is positive when the amplifiable reagent is amplified and the testing target is amplified, and determining that the testing target is absent or at least less than a specific copy number of the amplifiable reagent and a detection result is negative when the amplifiable reagent is amplified and the testing target is not amplified. Also provided are a detection determining device, a detection determining program, and a device used for the detection determining method.

Abstract: A complex (10) includes a liquid crystal component (13) and a support (11, 12) of the liquid crystal component (13), in which a lubricating interface derivation region (16) is formed between the liquid crystal component (13) and the support (11, 12). An optical element includes a pair of substrates (11, 12) having electrodes (18, 19) on at least one substrate (11), and a liquid crystal component (13) with which a space between the pair of substrates (11, 12) is filled, in which a lubricating interface derivation region (16) is formed between the pair of substrates (11, 12) and the liquid crystal component (13). It is preferable that the lubricating interface deriving agent (14) is present in the lubricating interface derivation region.

Abstract: Provided is a device including: a plurality of wells; a reagent composition located in each of the wells and containing an amplifiable reagent in a specific copy number; and two or more groups into which the wells are divided to have the amplifiable reagent located in the same specific copy number but to be varied in composition of the reagent composition except for the specific copy number. Preferably, composition except for the specific copy number of the amplifiable reagent includes at least any one of primer and amplifying reagent. More preferably, the device includes two or more groups varied in the specific copy number. Yet more preferably, the groups of wells include at least a negative control group in which the specific copy number of the amplifiable reagent is 0, and a group in which the specific copy number of the amplifiable reagent is close to the limit of detection.

Abstract: Provided is a detection determining device used in a detection determining method including, in detection of a testing target in a sample by amplification of the testing target and an amplifiable reagent, determining that the testing target is present and a detection result is positive when the amplifiable reagent is amplified and the testing target is amplified, and determining that the testing target is absent or at least less than a specific copy number of the amplifiable reagent and a detection result is negative when the amplifiable reagent is amplified and the testing target is not amplified, the detection determining device including at least one well, wherein the amplifiable reagent in the at least one well is contained in a specific copy number.

Abstract: A complex (10) includes a liquid crystal component (13) and a support (11, 12) of the liquid crystal component (13), in which a lubricating interface derivation region (16) is formed between the liquid crystal component (13) and the support (11, 12). An optical element includes a pair of substrates (11, 12) having electrodes (18, 19) on at least one substrate (11), and a liquid crystal component (13) with which a space between the pair of substrates (11, 12) is filled, in which a lubricating interface derivation region (16) is formed between the pair of substrates (11, 12) and the liquid crystal component (13). It is preferable that the lubricating interface deriving agent (14) is present in the lubricating interface derivation region.

Abstract: In one embodiment, provided are a method for analyzing at least one nucleic acid that can conveniently and highly accurately analyze even a very small number of analyte at least one nucleic acid. In one embodiment, the present invention relates to a method for analyzing at least one nucleic acid, comprising: a library preparation step of preparing a library comprising at least one standard nucleic acid of specific copy number(s) and at least one analyte nucleic acid in a same system; a calibration curve data generation step of generating calibration curve data based on the copy number(s) of the at least one standard nucleic acid of specific copy number(s); and an analyte nucleic acid analysis step of identifying at least one nucleotide sequence of the analyte nucleic acid while identifying the number(s) of the at least one nucleotide sequence of the at least one analyte nucleic acid using the calibration curve data.

Abstract: Provided is a nucleic acid sample-contained container including; a first nucleic acid molecule including an intended base sequence and a base sequence for detection different from the intended base sequence; and a second nucleic acid molecule free of the intended base sequence but including the base sequence for detection, wherein the nucleic acid sample-contained container includes the first nucleic acid molecule in a predetermined number. In a preferable mode, the copy number of the intended base sequence is less than 1,000, and the coefficient of variation (CV value) for the copy number is lower than 20%. In a more preferable mode, the nucleic acid molecules are artificially synthesized nucleic acid molecules. In a yet more preferable mode, the first nucleic acid molecule includes the intended base sequence in a plural number in the same molecule.

Abstract: Provided is a device including: at least one well in which an amplifiable reagent is contained in a specific copy number of less than 100; and at least one well in which the amplifiable reagent is contained in a specific copy number of 100 or greater, wherein for the at least one well in which the specific copy number of the amplifiable reagent is less than 100, a formula: CV<1/?x is established, where CV represents a coefficient of variation for the specific copy number and x represents average specific copy number of the amplifiable reagent.

Abstract: Provided is a detection accuracy identifying method for identifying a detection accuracy for detecting a testing target nucleic acid, the method including identifying a detection accuracy ability of the testing target nucleic acid, using a standard substance containing a known number of nucleic acid molecule and based on a probability at which the known number of nucleic acid molecule is detected.