Abstract: The present disclosure provides a biosensor chip including: a top cover including a sample loading slot for injecting blood sample, a glue injection hole, and a vent hole; a plasma separation membrane overlapping the sample slot; a conjugate pad overlapping the plasma separation membrane; an optical fiber; and a bottom cover including a conjugate pad groove for setting the conjugate pad, an optical fiber channel for setting the optical fiber, a glue injection groove corresponding to the position of the glue injection hole in the top cover, and a waste liquid tank, wherein the conjugate pad groove and the optical fiber channel are connected, the glue injection groove overlaps with the optical fiber channel, the vent hole is located on the waste liquid tank, and the upper cover further includes a separation membrane groove where the plasma separation membrane is set, and a flow channel connecting the optical fiber channel and the waste liquid tank.