Abstract: The present invention relates to a base station for a cleaning robot to park in, where the cleaning robot includes a wiping board, and a flexible wiping member replaceably butts the wiping board to form a wiping surface to wipe a working surface on which the cleaning robot walks, where the base station includes: a storage module, configured to store a continuous wiping base material; and a feeding module, configured to drive a free end of the wiping base material to be conveyed to a cutting position, to cause the free end to be cut from the wiping base material to form the wiping member. The present invention has the following beneficial effects: After returning to the base station, the cleaning robot may automatically mount a wiping member without intervention by a user.

Abstract: The present invention relates to the field of biotechnology, in particular to a DNA polymerase mutant and the use thereof. Compared with a wild type, the mutant provided in the present invention has a significantly improved DNA polymerization activity, significantly improved affinity to DNA and amplification uniformity; and has good effects in the amplification of multiplex PCR, high-GC templates and complex templates. A hot-start-version DNA polymerase is further prepared by means of using antibodies or chemical modifications, wherein the yield of the DNA polymerase is significantly improved, while the primer dimer is significantly decreased. The prepared mutant hot-start DNA polymerase can be applied to PCR amplification of multiplex PCR, high-GC templates and complex templates.

Abstract: Video coding methods, apparatuses, and non-transitory computer readable mediums are provided. The method includes: obtaining a list of reference pictures comprising a plurality of pictures stored in a buffer, where the plurality of pictures comprise at least a first picture with a first spatial resolution, and a second picture with a second spatial resolution different from the first spatial resolution; obtaining a reference picture from the list of reference pictures; predicting a current picture by using the reference picture with its original spatial resolution without ensuring the reference picture to have a same spatial resolution as the current picture; and generating a bitstream based on a predicted current picture.

Abstract: A control circuit of an isolated power supply, an isolated power supply and a control method thereof are disclosed. The control circuit includes a primary original signal generator generating a primary original turn-on signal based on a voltage feedback signal of an output voltage of the isolated power supply, and a secondary control signal generator generating a secondary control signal based on a secondary voltage signal and the primary original turn-on signal. In a current switching period, only when an expected turn-on instant for a primary main transistor switch is earlier than an expected turn-on instant for a secondary synchronous rectifier, will the secondary control signal provide an indication to turn on the secondary synchronous rectifier.

Abstract: Provided is a recombinant KOD polymerase, which is the following A) or B): the polymerase shown in A) is a protein having DNA polymerase activity that is obtained by modifying amino acid residues in at least one of the following 18 positions in a wild-type KOD DNA polymerase amino acid sequence: 675th, 385th, 710th, 674th, 735th, 736th, 606th, 709th, 347th, 349th, 590th, 676th, 389th, 589th, 680th, 384th, 496th and 383rd; the polymerase described by B) is a protein having DNA polymerase activity that is derived from A) by adding a tag sequence to an end of the amino acid sequence of the protein shown in A).

Abstract: The present invention relates to a base station for a cleaning robot to park in, where the cleaning robot includes a wiping board, and a flexible wiping member replaceably butts the wiping board to form a wiping surface to wipe a working surface on which the cleaning robot walks, where the base station includes: a storage module, configured to store a continuous wiping base material; and a feeding module, configured to drive a free end of the wiping base material to be conveyed to a cutting position, to cause the free end to be cut from the wiping base material to form the wiping member. The present invention has the following beneficial effects: After returning to the base station, the cleaning robot may automatically mount a wiping member without intervention by a user.

Abstract: Provided is a recombinant KOD polymerase. A KOD polymerase mutant is a protein as follows: the protein is a protein having DNA polymerase activity that is obtained by modifying amino acid residues in at least one of the following 48 positions in the amino acid sequence of KOD DNA polymerase GH78: 267, 326, 347, 349, 353, 375, 378, 379, 380, 385, 451, 452, 453, 454, 457, 461, 465, 470, 474, 477, 478, 479, 480, 482, 484, 485, 486, 493, 496, 497, 514, 574, 584, 605, 610, 630, 665, 666, 667, 674, 676, 680, 682, 698, 707, 718, 723 and 729, without changing other amino acid sequences; and compared with KOD DNA polymerase GH78, with regard to catalysis, the recombinant DNA polymerase exhibits a faster reaction rate, better catalytic efficiency, better affinity and other advantages, thereby improving the reaction rate of DNA polymerase in sequencing and increasing the reaction read length.

Abstract: Provided are a thermostable B-family DNA polymerase mutant and an application thereof. The thermostable B-family DNA polymerase mutant is provided to mutate amino acid residues in at least two of three sites of group A, i.e., 408, 409, and 410 in an amino acid sequence of thermostable B-family DNA polymerases to obtain proteins having DNA polymerase activity. A-group sites (408/409/410), B-group sites (451/485), C-group sites (389/383/384), D-group sites (589/67/680), E-group sites (491/493/494/497), and F-group sites (474/478/486/480/484) of several B-family DNA polymerases are analyzed to design the mutant.

Abstract: A control circuit and method for an isolated switching-mode converter are disclosed. The control circuit includes a primary-side controller and a secondary-side controller. The primary-side controller can enter a sleep mode in response to a first request signal, and the primary-side controller can turn on or off a first switch in the primary-side circuit in a predefined manner to cause a secondary-side winding in a transformer to generate a predefined first output signal. The secondary-side controller can detect the first output signal and responsively enter the sleep mode. In this way, both the primary-side and secondary-side controllers can enter the sleep mode, thus significantly reducing standby power dissipation of the system.

Abstract: The present invention provides a switched-mode power converter. A control circuit in the switched-mode power converter includes first and second control chips and a magnetic coupling chip coupled to both the former two. The magnetic coupling chip provides a galvanically isolated communication link between the first control chip and the second control chip. With the aid of the second control chip and the magnetic coupling chip, the control circuit can provide a feedback signal to the first control chip and thereby act on turn-on and turn-off of a switching device in the switching circuit. In this way, desirable output and isolation performance can be achieved. Since the first control chip, the second control chip and the magnetic coupling chip can be integrated at a high degree of integration, the switched-mode power converter can have a high degree of integration, reduced size and lower cost.

Abstract: A mechanical continuously variable transmission (CVT) that can store energy in mechanical form simultaneously, so that the vehicle kinetic energy during the last braking can be used for the next starting acceleration, obtaining unexpected energy-saving effects.

Abstract: The invention provides a sensor device comprising an insect odorant receptor (OrX) in electrical communication with a substrate, wherein the sensor device is configured to detect a change in an electrical characteristic of the substrate. The invention also provides sensor device component comprising an insect odorant receptor (OrX) in electrical communication with a substrate. The invention also provides methods for manufacture and use of the sensor device and sensor device component. The invention also provides methods of use of the sensor to detect an analyte.

Abstract: Methods and endpoint nodes and controllers are disclosed for mutual authentication and key exchange. In an embodiment, physical unclonable function circuits on the endpoint nodes are used in combination with key masks to allow mutual authentication and key exchange between the endpoint nodes.

Abstract: The invention provides a sensor device comprising an insect odorant receptor (OrX) in electrical communication with a substrate, wherein the sensor device is configured to detect a change in an electrical characteristic of the substrate. The invention also provides sensor device component comprising an insect odorant receptor (OrX) in electrical communication with a substrate. The invention also provides methods for manufacture and use of the sensor device and sensor device component. The invention also provides methods of use of the sensor to detect an analyte.

Abstract: A chimeric DNA polymerase includes: a first fragment having at least 80% homology to at least part of an N-end domain of KOD DNA polymerase; a second fragment having at least 80% homology to at least part of an exonucleolytic domain of Pab DNA polymerase; a third fragment having at least 80% homology to at least part of the N-end domain of KOD DNA polymerase; a fourth fragment having at least 80% homology to at least part of a palm domain of Pfu DNA polymerase; a fifth fragment having at least 80% homology to at least part of a finger domain of Pab DNA polymerase; a sixth fragment having at least 80% homology to at least part of the palm domain of Pfu DNA polymerase; and a seventh fragment having at least 80% homology to at least part of a thumb domain of KOD DNA polymerase.

Abstract: In an embodiment, a method (100) is described. The method comprises receiving (102) data corresponding to a plurality of radiographic imaging slices of a body. The method further comprises determining (104) a position of a needle inserted in the body. The determination is based on combining information from at least one of the radiographic imaging slices comprising an indication of a first portion of the needle outside the body and at least one other of the radiographic imaging slices comprising an indication of a second portion of the needle inside the body. A combined needle region is generated by merging data corresponding to a position of the first portion of the needle outside the body with data corresponding to a position of the second portion of the needle inside the body.

Abstract: The invention provides a detection circuit for detecting light-off modes performed by a silicon-controlled dimmer which comprises a voltage detection circuit receiving an output signal and generating a voltage detection signal according to the output signal, and a delay circuit connected to the voltage detection circuit, receiving the voltage detection signal, and delaying the voltage detection signal in order to output a detection signal. The invention detects the output signal through the voltage detection circuit, and delays the detected voltage detection signal to output the corresponding detection signal, and then the detection signal effectively distinguishes the light-off modes performed by the silicon-controlled dimmer to meet requirements of users.