Abstract: The present invention is directed to an integrated conceptual strategy for a gene delivery system, using the combination of nanoparticles, CRISPR-Cas9, and the HITI strategy to deliver CRISPR-Cas9 and achieve effective genome editing; wherein the advanced nanoparticles to overcome the limited packaging size of AAV-based vehicles.

Abstract: The preset invention relates to a novel super-enhancer-bound Ash2l/OSN complex that can drive enhance activation, govern pluripotency network and sternness circuitry, and a reprogramming system or method through the regulation of this super-enhancer, Ash2l, to modulate pluripotency and cell fates. Ash2l directly binds to super-enhancers of several stemness genes to regulate pluripotency and self-renewal in pluripotent stem cells. Ash2l recruits Oct4/Sox2/Nanog (OSN) to form Ash2l/OSN complex at the super-enhancers of Jarid2, Nanog, Sox2, and Oct4, and further drives enhancer activation, upregulation of stemness genes, and maintains the pluripotent circuitry. Ash2l knockdown abrogates the OSN recruitment to all super-enhancers and further hinders the enhancer activation.

Abstract: The present disclosure pertains to a method for diagnosing AMD comprising receiving OCTA image of a subject, pre-processing the OCTA image to obtain image data, inputting the image data to a trained deep learning (DL) network, generating using the trained DL network an output that characterizes the health of the subject with respect to AMD, and generating a diagnostic result based on the output.

Abstract: The preset invention relates to a novel super-enhancer-bound Ash2l/OSN complex that can drive enhance activation, govern pluripotency network and stemness circuitry, and a reprogramming system or method through the regulation of this super-enhancer, Ash2l, to modulate pluripotency and cell fates. Ash2l directly binds to super-enhancers of several stemness genes to regulate pluripotency and self-renewal in pluripotent stem cells. Ash2l recruits Oct4/Sox2/Nanog (OSN) to form Ash2l/OSN complex at the super-enhancers of Jarid2, Nanog, Sox2, and Oct4, and further drives enhancer activation, upregulation of stemness genes, and maintains the pluripotent circuitry. Ash2l knockdown abrogates the OSN recruitment to all super-enhancers and further hinders the enhancer activation. In addition, CRISPRi/dCas9-mediated blocking of Ash2l-binding motifs at these super-enhancers also prevents OSN recruitment and enhancer activation, validating that Ash2l directly binds to super-enhancers and initiates the pluripotency network.

Abstract: The present invention relates to a novel method for preparing induced pluripotent stem cells (iPSCs) by introducing three genes, Oct3/4, Sox2, and Parp1, into somatic cells. The present invention also relates to the iPSCs produced by the aforementioned method. Also provided is a method of rejuvenating cells by use of a PARylated protein or an enzyme with PARylation activity. Further provided is a method for inducing the secretion of interferon-? inducible protein-10 (IP-10) comprising administering to a subject in need thereof an effective amount of iPSCs or iPSC-CM.

Abstract: This invention provides a device combining a slim CD-ROM drive and a flash memory card drive, which utilizes the unused space in a slim CD-ROM drive to mount a flash memory card socket to form a combined device. It comprises of a seat with the first containing space and the second containing space. The first containing space hosts a circuit board and a driving unit, a standard CD-ROM drive with a panel on the front and the panel has a opening against the second containing space, a flash memory socket in the second containing space and its slot faces against the said opening of the panel. The socket can hold multi types of flash memory cards, including SD, MMC, and MS cards. Furthermore, there are multi PINs in the socket and they are connected to the circuit board on one end and contact the flash memory card on the other end to support access to the flash memory card.

Abstract: The present invention discloses a high-speed serial bus power supply device to connect a 4-PIN cable receptacle and a USB interface socket on a notebook. It includes a first connector, which is a common 6-PIN cable receptacle and can be connected to an appropriate interface; a second connector, which is a 4-PIN cable plug connected to the first connector and can be inserted into the said 4-PIN cable receptacle of the notebook; and a USB plug, which is also connected to the first connector and can deliver power to the serial port through said USB interface socket which is connected to the notebook.

Abstract: This invention provides a device combining a slim CD-ROM drive and a flash memory card drive, which utilizes the unused space in a slim CD-ROM drive to mount a flash memory card socket to form a combined device. It comprises of a seat with the first containing space and the second containing space. The first containing space hosts a circuit board and a driving unit, a standard CD-ROM drive with a panel on the front and the panel has a opening against the second containing space, a flash memory socket in the second containing space and its slot faces against the said opening of the panel. The socket can hold multi types of flash memory cards, including SD, MMC, and MS cards. Furthermore, there are multi PINs in the socket and they are connected to the circuit board on one end and contact the flash memory card on the other end to support access to the flash memory card.