Abstract: An allulose 3-epimerase mutant, a genetically engineered bacterium expressing the mutant, and an immobilized allulose 3-epimerase enzyme and an immobilization method thereof are described. A high-throughput screening method is used to obtain an allulose 3-epimerase mutant efficiently expressed in a fermentation process, which can catalyze efficient conversion of fructose to D-allulose, providing an efficient production path for key enzymes required in a D-allulose production process. Additionally, the allulose 3-epimerase is bonded to an immobilizing resin to prepare an immobilized allulose 3-epimerase enzyme. The immobilized enzyme can be applied to batch or continuous reactions to catalyze efficient conversion of fructose to D-allulose.

Abstract: The application provides a gas density relay with online self-check function and its check method, including a gas density relay body, a first pressure sensor, a second pressure sensor, a temperature sensor, a gas chamber, a pressure regulating mechanism, an online check contact signal sampling unit and an intelligent control unit. The air path of the pressure regulating mechanism is connected to the gas pressure chamber and the second pressure sensor; Pressure rise and fall can be regulated through the pressure regulating mechanism to make the gas density relay body contact action. The contact action is transmitted to the intelligent control unit through the online check contact signal sampling unit. The intelligent control unit detects the action value and/or return value of the contact signal of the gas density relay body according to the density value when the contact acts, and completes the check work without requiring maintainer to go to the site for check.

Abstract: Recombinant strains are obtained for the production of allulose, allose, and allitol by regulating intracellular glucose metabolism, reducing the enzyme activity of fructose 6-phosphate kinase, and enhancing the enzyme activities of glucokinase and glucose-6-phosphate isomerase, allulose 6-phosphate 3-epimerase, allulose 6-phosphate phosphatase, fructose permease and fructokinase, and optionally enhancing the enzyme activities of ribose 5-phosphate isomerase, allose 6-phosphate phosphatase, ribitol dehydrogenase, glycerol permease, glycerol dehydrogenase, and dihydroxyacetone kinase. A method for producing allulose and allose is an extracellular multienzyme cascade method. Multienzyme cascade catalysis and fermentation are coupled to improve the conversion rate of starch sugar or sucrose to the synthesized allulose.

Abstract: Disclosed is a rotating cored-type biological treatment device for wastewater with low carbon content and use thereof. The device includes a composite frame (1), several biological packing columns (2), a rotating tray (3), a rotating shaft, and a base (4), wherein the biological packing columns (2) are detachably mounted inside the composite frame (1); the rotating shaft is vertically arranged on the base (4) and can rotate; the rotating tray (3) is fixedly sheathed on the rotating shaft, and the composite frame (1) is disposed on the rotating tray (3); and the rotating tray rotates with the rotating shaft, which driving the composite frame (1) and the biological packing columns (2) therein to rotate. The rotating cored-type biological treatment device has the advantages of high efficiency and being renewable and environment-friendly, etc.

Abstract: The application provides a gas density relay with online self-check function and its check method, including a gas density relay body, a first pressure sensor, a second pressure sensor, a temperature sensor, a gas chamber, a pressure regulating mechanism, an online check contact signal sampling unit and an intelligent control unit. The air path of the pressure regulating mechanism is connected to the gas pressure chamber and the second pressure sensor; Pressure rise and fall can be regulated through the pressure regulating mechanism to make the gas density relay body contact action. The contact action is transmitted to the intelligent control unit through the online check contact signal sampling unit. The intelligent control unit detects the action value and/or return value of the contact signal of the gas density relay body according to the density value when the contact acts, and completes the check work without requiring maintainer to go to the site for check.

Abstract: A method of treating a cancer in a mammalian subject, where the cancer has multi-therapy resistance, comprises administering to the mammalian subject a therapeutically effective amount of a pentaaza macrocyclic ring complex corresponding to the Formula (I) below, optionally with administration of a further anti-cancer therapy.

Abstract: A method of treating a cancer in a mammalian subject with a tumor signature characterized by any one or more of (i) a level of sirtuin (SIRT3) protein that is below a first predetermined threshold level, (ii) a level of manganese superoxide dismutase acetylated at the lysine 68 residue (AcK68) that exceeds a second predetermined threshold level, and (iii) expression levels of hypoxia-inducible factor 2? (HIF2?) that exceed a third predetermined threshold level indicative of lineage plasticity for stemness, the method comprising administering to the mammalian subject a therapeutically effective amount of a pentaaza macrocyclic ring complex corresponding to the Formula (I) below, optionally with administration of a further anti-cancer therapeutic agent.

Abstract: An allulose 3-epimerase mutant, a genetically engineered bacterium expressing the mutant, and an immobilized allulose 3-epimerase enzyme and an immobilization method thereof are described. A high-throughput screening method is used to obtain an allulose 3-epimerase mutant efficiently expressed in a fermentation process, which can catalyze efficient conversion of fructose to D-allulose, providing an efficient production path for key enzymes required in a D-allulose production process. Additionally, the allulose 3-epimerase is bonded to an immobilizing resin to prepare an immobilized allulose 3-epimerase enzyme. The immobilized enzyme can be applied to batch or continuous reactions to catalyze efficient conversion of fructose to D-allulose.

Abstract: The present invention discloses a highly effective in situ treatment method for endogenous pollution in sludge, belonging to the field of environmental engineering water treatment. In view of the problem that it is difficult to effectively treat the endogenous pollution of river sludge, the present invention provides the highly effective in situ treatment method for endogenous pollution in sludge, which specifically adopts a combination of phoslock, calcium peroxide and aluminum modified zeolite to perform in-situ remediation of sludge, effectively inhibit the release of endogenous pollution of sludge, facilitate the ecological treatment of polluted water subsequently, and finally realize the long-term water improvement against pollution.

Abstract: Disclosed is a rotating cored-type biological treatment device for wastewater with low carbon content and use thereof. The device includes a composite frame (1), several biological packing columns (2), a rotating tray (3), a rotating shaft, and a base (4), wherein the biological packing columns (2) are detachably mounted inside the composite frame (1); the rotating shaft is vertically arranged on the base (4) and can rotate; the rotating tray (3) is fixedly sheathed on the rotating shaft, and the composite frame (1) is disposed on the rotating tray (3); and the rotating tray rotates with the rotating shaft, which driving the composite frame (1) and the biological packing columns (2) therein to rotate. The rotating cored-type biological treatment device has the advantages of high efficiency and being renewable and environment-friendly, etc.

Abstract: Recombinant strains are obtained for the production of allulose, allose, and allitol by regulating intracellular glucose metabolism, reducing the enzyme activity of fructose 6-phosphate kinase, and enhancing the enzyme activities of glucokinase and glucose-6-phosphate isomerase, allulose 6-phosphate 3-epimerase, allulose 6-phosphate phosphatase, fructose permease and fructokinase, and optionally enhancing the enzyme activities of ribose 5-phosphate isomerase, allose 6-phosphate phosphatase, ribitol dehydrogenase, glycerol permease, glycerol dehydrogenase, and dihydroxyacetone kinase. A method for producing allulose and allose is an extracellular multienzyme cascade method. Multienzyme cascade catalysis and fermentation are coupled to improve the conversion rate of starch sugar or sucrose to the synthesized allulose.

Abstract: The present invention discloses a highly effective in situ treatment method for endogenous pollution in sludge, belonging to the field of environmental engineering water treatment. In view of the problem that it is difficult to effectively treat the endogenous pollution of river sludge, the present invention provides the highly effective in situ treatment method for endogenous pollution in sludge, which specifically adopts a combination of phoslock, calcium peroxide and aluminum modified zeolite to perform in-situ remediation of sludge, effectively inhibit the release of endogenous pollution of sludge, facilitate the ecological treatment of polluted water subsequently, and finally realize the long-term water improvement against pollution.

Abstract: The invention provides compositions and methods to treat cancer with an agent that increases reactive oxygen species (ROS) levels in cancer cell mitochondria (“an XTPP agent”) or a pharmaceutically acceptable salt thereof, an inhibitor of hydroperoxide metabolism and a pharmaceutically acceptable diluent or carrier.

Abstract: The invention provides compositions and methods to treat cancer with an agent that increases reactive oxygen species (ROS) levels in cancer cell mitochondria (“an XTPP agent”) or a pharmaceutically acceptable salt thereof, an inhibitor of hydroperoxide metabolism and a pharmaceutically acceptable diluent or carrier.